SN/T 4545.2-2016 English PDF

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SN/T 4545.2-2016: Commercial kit method - Salmonella - Test method II
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4545.2-2016	229	Add to Cart	3 days	Commercial kit method - Salmonella - Test method II	Valid

Similar standards

GB/T 687   GB/T 693   SN/T 4545.4   SN/T 4545.3   SN/T 4545.1   

Basic data

Standard ID: SN/T 4545.2-2016 (SN/T4545.2-2016)
Description (Translated English): Commercial kit method - Salmonella - Test method II
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: G66
Word Count Estimation: 10,192
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 4545.2-2016: Commercial kit method - Salmonella - Test method II

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Commercial kit method-Salmonella-Test method II People's Republic of China Exit Inspection and Quarantine standards Commercial kit detection method Salmonella method two 2016-08-23 released 2017-03-01 Implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This part is the second part of SN/T 4545. This section drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of this document may be patentable. The release of this document The agency does not assume responsibility for identifying these patents. This part of the national certification and accreditation supervision and management committee proposed and centralized. This part of the drafting unit. People's Republic of China Beijing Exit Inspection and Quarantine, People's Republic of China Fujian Entry-Exit Inspection and Quarantine Epidemic The main drafters of this section. Zeng Jing, Zhang Meng, Pu Pu, Han laugh, Wang Ziwei, Wang Qi, Zheng Jing. Commercial kit detection method Salmonella method two

1 Scope

SN/T 4545 provisions of this part of the food salmonella chromogenic culture screening and rapid detection of latex agglutination. This section applies to the qualitative detection of Salmonella in food. This section of the application of color screening medium can detect all serotypes of Salmonella; latex agglutination reaction is only applicable to group A and Salmonella outside the F group.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 4789.4 National Food Safety Standard Microbiological Tests for Salmonella GB 19489 laboratory biosafety common requirements GB/T 27403 laboratory quality control specification food molecular biology test SN/T 2775 commercial food testing kit evaluation method SN/T 3266 Microbiological test methods for the validation of technical specifications

3 Biosecurity measures

To protect the safety of laboratory personnel and prevent contamination, Salmonella should be tested by qualified staff. All cultures, DNA extracts and waste should be in accordance with GB 19489 and GB/T 27403 in the implementation of the relevant provisions.

4 method principle

First, the use of chromogenic screening medium to determine the presence of Salmonella, chromogenic screening medium added C8 esterase and β-D glucose Glycosidase, C8 esterase Salmonella bacteria appear within 24h colonies, β-D-glucosidase non-Salmonella in other colors, you can pass Colony color to determine. Then, latexagglutination test was carried out to test the effect of the addition of part A group and F group Salmonella confirmed that the latex particles were coated with specific antigens against Salmonella other than part of group A and group F Polyvalent antiserum can be judged by whether agglutination of non-A and F group Salmonella, part of the group A and F group Salmonella need to enter One-step experiment confirmed.

5 Reagents and materials

5.1 Salmonella rapid test kit The kit reference SN/T 2775 and SN/T 3266 by the CNCA commodity food testing kit Evaluation Committee of Experts Group Weaving evaluation. Specific evaluation results see Appendix A. The kit is composed as follows. ......

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