SN/T 4278-2015 English PDF

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SN/T 4278-2015: Protocol for DNA barcoding identification techniques of medical insect at frontier ports
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Basic data

Standard ID: SN/T 4278-2015 (SN/T4278-2015)
Description (Translated English): Protocol for DNA barcoding identification techniques of medical insect at frontier ports
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C62
Classification of International Standard: 07.080
Word Count Estimation: 20,251
Date of Issue: 2015-05-26
Date of Implementation: 2016-01-01
Quoted Standard: GB/T 6682; GB 19489; SN/T 1876; SN/T 2752.4; WS/T 230
Regulation (derived from): State Quality-Inspection-accreditation [2015] No.224
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the medical insect vector DNA barcoding of sample processing, gene amplification, sequence analysis, identification and determination than the results of other operations procedures and norms. This standard applies to the inspection and quarantine agencies in border crossings using DNA barcoding for adults (including limbs mutilated adult) and the type of appraisal pupae, larvae and eggs and other forms can not rely on direct identification of medical vector insects.

SN/T 4278-2015: Protocol for DNA barcoding identification techniques of medical insect at frontier ports

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Border crossings insect vector DNA barcoding Medical Practice) People's Republic of China Entry-Exit Inspection and Quarantine Standards Border crossings medical vector DNA barcoding insect Identification procedures atfrontierports Issued on. 2015-05-26 2016-01-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, People's Republic of China Hebei Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Yueqiao Yun, Qiu Deyi, Nie Weizhong, LI Yun-song, Chen Jian, Liu Xing, Wei Xiaoya, Wu scalability, Hu Jia.

Introduction

DNA barcoding is a recently developed based on the establishment of a new species identification technology in gene amplification and sequence alignment Surgery, the use of a living body to represent the species standard, there is enough variation, easy and relatively short amplified DNA fragment species Identification. The technology is not the state of development of the species, male and female individuals and morphological integrity or not restrictions, with fast, accurate, low cost Features. In 2003, Canadian researchers found that PaulHebert mitochondrial cytochrome C oxidase subunit I (COI) gene capable of providing A fast, simple and credible classification method that can accurately distinguish species, and the first time DNA barcoding (DNABarcoding) of concept. In 2010, co-founded by the 26 countries of the International Consortium for the Barcode of Life (iBOL), we are committed to develop into DNA barcoding General an efficient and accurate way to identify the species, the world has more than 50 countries to participate in this alliance, the purpose is to build a global object Molecular species identification platform. BOLD Barcode Data Systems (Barcodingoflifedatasystem) as an international organization of bar codes Dedicated database and identification platform, screening and voucher specimens DNA barcoding has strict requirements, such as. must have a unique series of samples No details, storage, identification and collection (collection of people, collecting latitude and longitude location with GPS location information), require gene fragments will It must be greater than 500bp, to provide the use of PCR primer sequences, sequence map, the original sequence or other tracking files. Currently the system is BOLD After more than 200,000 kinds of organisms contain DNA barcode sequence, and are at a very fast pace. For medical media insect identification relates to border health security, the general use of a length of approximately 658bp for COI gene Standard DNA barcode sequences with BOLD, NCBI database system for comparison. The main drafters DNA barcoding has been engaged in the work of the laboratory in recent years has been the primitive accumulation of more than 300 kinds of media Insect-mediated DNA barcode data more than 5000, has a wealth of technology for the use of DNA barcoding to identify species of experience. This standard is based on the International Consortium for the Barcode of Life (iBOL) for DNA barcoding requirements, combining the characteristics of the media monitoring of border crossings Developed to promote and standardize the inspection and quarantine agencies throughout the country using DNA identification laboratory medicine vector insects barcode technology operations, So as to achieve the purpose of obtaining accurate and reliable results. Border crossings medical vector DNA barcoding insect Identification procedures

1 Scope

This standard specifies the medical insect vector DNA barcoding of sample processing, gene amplification, sequence analysis, comparison and identification of junction If it is determined and other operational procedures and norms. This standard applies to the inspection and quarantine agencies in border crossings using DNA barcoding for adults (including limbs mutilated adults) Identification work and pupae, larvae and eggs and other forms can not rely on direct identification of medical vector insects.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety SN/T 1876 medical vectors specimen collection, production and preservation regulations SN/T 2752.4 specification of self-protection of the health and quarantine personnel - Part 4. Laboratory personnel Applications WS/T 230 clinical diagnostic polymerase chain reaction (PCR) technology

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 DNA barcoding DNAbarcode To represent the living body of the species, the standard, there is enough variation, and relatively easy to amplify short DNA fragments. DNA barcoding Species identification code has become an important tool in medical vector insects, generally refers to mitochondrial COI (cytochrome C oxidase Standard 658bp fragment of subunit I) on. 3.2 DNA barcoding technology DNAbarcodingidentificationtechnique A use of DNA barcoding gene fragment species identification and phylogenetic studies of the relationship between technology, based on gene amplification And on the basis of sequence alignment, it has not species developmental status, male and female individuals and morphological integrity or not to limit, to reduce Experts dependence was not affected by subjective factors, high accuracy, using the Internet can achieve data sharing and so on. 3.3 Voucher specimens voucherspecimen Get molecules such as DNA barcoding source data (the parent) specimens. Voucher specimens may be incomplete specimen form, but this standard The molecules of DNA barcode credentials and other data must be a one to one relationship. For the very small sample size (such as mites, etc.), may be The same batch of the same species in a sample of individuals. Save the voucher specimen is very important, it must have a unique number of samples, storage, identification and Mining For more information about sets, etc., in order to facilitate future verification. ......

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