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SN/T 4260-2015: Determination of crude polysaccharides in plant source foods for export. Phehol-sulfuric acid colorimetry Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedure Status: Valid
SN/T 4260-2015: Determination of crude polysaccharides in plant source foods for export. Phehol-sulfuric acid colorimetry---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/SNT4260-2015 ENTRY-EXIT INSPECTION AND QUARANTINE INDUSTRY STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA Determination of crude polysaccharides in plant source foods for export - Phenol-sulfuric acid colorimetry ISSUED ON: MAY 26, 2015 IMPLEMENTED ON: JANUARY 01, 2016 Issued by: General Administration of Quality Supervision, Inspection and Quarantine of PRC Table of ContentsForeword ... 3 1 Scope ... 4 2 Normative references ... 4 3 Terms and definitions... 4 4 Method summary ... 5 5 Reagents and materials ... 5 6 Instruments and equipment ... 5 7 Sample preparation and storage ... 6 8 Measurement steps ... 6 9 Result calculation ... 7 10 Repeatability ... 8 Appendix A (Informative) Qualitative identification of starch and dextrin ... 9 Determination of crude polysaccharides in plant source foods for export - Phenol-sulfuric acid colorimetry1 ScopeThis standard specifies a colorimetric method, for the determination of crude polysaccharides in plant source foods for export. This standard is applicable to the determination of crude polysaccharide content in edible fungi, wolfberry, grapes, dates, fruit juices and other plant source foods. This standard does not apply to foods, to which starch and dextrin components are added. The detection limit of this standard method is 0.5 mg/kg.2 Normative referencesThe following documents are essential to the application of this document. For the dated documents, only the versions with the dates indicated are applicable to this document; for the undated documents, only the latest version (including all the amendments) is applicable to this standard. GB/T 6682-2008 Water for analytical laboratory use - Specification and test methods3 Terms and definitionsThe following terms and definitions apply to this document. 3.1 Crude polysaccharides A series of polymer compounds, which use β-D-glucan or α-D-glucan or other carbon sugars, as the main chain. The general molecular formula is (C6H12O6)n.7 Sample preparation and storage7.1 Determination of the presence of starch and dextrin in the sample Refer to Appendix A for judgment. If the sample contains starch and dextrin, this method is not applicable to the determination of polysaccharide content in this sample. If the sample does not contain starch and dextrin, proceed to the next determination step. 7.2 Specimen preparation 7.2.1 Preserved fruit samples such as dried wolfberry, raisins, dried apricots Place the solid sample to be tested, in a frozen state for drying and then pulverized. After pulverization, the sample is passed through a 20 mm sieve AND mixed evenly. 7.2.2 Mushroom samples such as shiitake mushrooms, oyster mushrooms, Ganoderma lucidum Dry the sample to be tested. Pass it through a 20 mm sieve after crushing. Mix it evenly. 7.2.3 Fruit samples such as grapes and jujubes Peel and de-nucleate the sample to be tested. Dry and pulverize it. Pass it through a 20 mm sieve. Mix it evenly. 7.3 Sample preservation The samples are stored in -18 °C refrigerator. During sample preparation and sample preservation, contamination of samples and loss of analytes shall be prevented.8 Measurement steps8.1 Sample processing Weigh 0.2 g ~ 1.0 g of the sample, accurate to 0.001 g, into a 50 mL stoppered centrifuge tube. Immerse the sample in 5 mL of water. Slowly add 20 mL of anhydrous ethanol. Shake it by a vortex oscillator, to make the mixture uniform. Place it in an ultrasonic extractor for ultrasonic extraction for 30 min. After the extraction, centrifuge at 4000 r/min for 10 min. Discard the supernatant. The insoluble material is washed by 10 mL of 80% ethanol solution, and centrifuged. Use water to transfer the above insoluble matter into a round-bottomed flask. Add 50 mL of water. Perform ultrasonic extraction at 120 W for 30 min; repeat these operations twice. Cool it to room temperature. Filter it. Transfer the supernatant into a 200 mL volumetric flask. Wash the residue 2 ~ 3 times. Transfer the washing solution to a volumetric flask. Add water to make its volume reach to the mark. This solution is the sample measurement solution (if the color is too deep, it can be decolorized by C18SPE small column, etc.). ......Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al. Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of English version of SN/T 4260-2015 be delivered?Answer: The full copy PDF of English version of SN/T 4260-2015 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice.Question 2: Can I share the purchased PDF of SN/T 4260-2015_English with my colleagues?Answer: Yes. 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