SN/T 4097-2015 English PDF

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SN/T 4097-2015: Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4097-2015	189	Add to Cart	3 days	Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish	Valid

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Basic data

Standard ID: SN/T 4097-2015 (SN/T4097-2015)
Description (Translated English): Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 07.080
Word Count Estimation: 8,829
Date of Issue: 2015-02-09
Date of Implementation: 2015-09-01
Quoted Standard: GB/T 6682
Regulation (derived from): State-Quality-Inspection-Accreditation [2015] 59
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the real-time PCR detection method shellfish centrist piano insects. This standard applies to shellfish tissue centrist piano insect detection.

SN/T 4097-2015: Real-time fluorescent PCR quarantine protocol for Perkinsus sp.in shellfish

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Shellfish piano insect send real-time PCR detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Shellfish send piano insect real-time PCR detection method Issued on. 2015-02-09 2015-09-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters. Zhang Xue, Zhao Huijun, Li Ye, LI Zhen-Rong, Jia Yun. Shellfish send piano insect real-time PCR detection method

1 Scope

This standard specifies the real-time PCR detection method shellfish centrist piano insects. This standard applies to shellfish tissue centrist piano insect detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Real-time fluorescence PCR real-timefluorescentPCR On the basis of the ordinary on the polymerase chain reaction to add a specific fluorescent probe which is an oligonucleotide labeled at both ends of a Reports a fluorophore and quencher fluorophore. When the probe is intact, the fluorescence signal emitted by the reporter group is absorbed by the quencher; PCR Amplification, Taq polymerase 5'-3 'exonuclease activity of the enzyme degradation of the probe, so that the reporter group and quencher separate fluorescence monitoring system in order to Receiving the fluorescence signal, per amplify a DNA strand, there is formed a fluorescent molecule, a cumulative PCR product shaped fluorescent signal A fully synchronized, real-time quantitative achieve the purpose. 3.2 Ct values cyclethreshold When the number of cycles for each reaction tube fluorescent signal reaches the threshold set by experienced, also known as Cp values.

4 Reagents and materials

4.1 1mol/LTris · Cl (pH8.0), 0.5mol/LEDTA solution (pH8.0), SDS (10%). Solution preparation see Appendix A. 4.2 proteinase K solution (20mg/mL). 4.3 Tris- saturated phenol. 4.4 chloroform. 4.5 isoamyl alcohol. 4.6 ethanol. 4.7 saline. 4.8 10 × PCRbuffer, 25mmol/LMgCl2,2.5mmol/LdNTP, 5U/μLTaq enzyme. 4.9 10μmol/L upstream primer, 10μmol/L downstream primer, 10μmol/L probe. 4.10 TE buffer (pH8.0). ......

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