SN/T 4077-2014 English PDF
Basic dataStandard ID: SN/T 4077-2014 (SN/T4077-2014)Description (Translated English): (Odontoglossum ringspot virus quarantine and identification methods) Sector / Industry: Commodity Inspection Standard (Recommended) Date of Issue: 12/1/2014 Date of Implementation: 5/1/2015 Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the molecular biology and immunology methods Odontoglossum ringspot virus quarantine identified. This standard applies to quarantine and identification of orchid seedlings and plant products may toothed ringspot virus. SN/T 4077-2014: (Odontoglossum ringspot virus quarantine and identification methods)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Odontoglossum ringspot virus quarantine and identification methods) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Method for quarantine and identification of tooth ring spot virus Released on.2014-11-19 2015-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Xiamen Plant Protection and Quarantine Station. The main drafters of this standard. Chen Qing, Huang Feng, Li Bin, Liao Furong, Xie Yizhen, Chen Hongyun, Lin Shiming. Method for quarantine and identification of tooth ring spot virus1 ScopeThis standard specifies the immunological and molecular biological detection methods for quarantine identification of tooth ringworm. This standard applies to the quarantine and identification of orchid seedlings and plant products that may have tooth ring spot virus.2 tooth ring spot virus basic informationScientific name. odontoglossumringspotvirus Abbreviation. ORSV Taxonomic status. a member of the Tobamovirus. ORSV mainly transmits through mechanical transmission, and peach aphid can transmit drugs. See Appendix A for details.3 Principle of the methodAccording to the specific reaction between the tooth ring virus and the antibody, the orchid leaves were tested by ELISA; according to the virus genome The specificity of PCR was detected, and the results were determined by the size of the electrophoresis strip. 4 instruments, facilities and reagents 4.1 Instruments and facilities Enzyme-linked detector, electronic balance (inductive quantity 0.0001g), PCR instrument, electrophoresis instrument, electrophoresis tank, gel imaging system, constant temperature water bath, low temperature ice Box, ordinary refrigerator, centrifuge, etc.; micropipette (2.5μL, 10μL, 20μL, 100μL,.200μL, 1000μL); enzyme plate, mortar Etc; anti-insect greenhouse. 4.2 Reagents Enzyme-linked detection reagents (see Appendix B), RT-PCR detection reagents (see Appendix C), IC-RT-PCR detection reagents (see Appendix D).5 Quarantine and identification5.1 Sample preparation The samples were observed and the seedling numbers with symptoms (eg, leaf deformity, mosaic, mottle, etc.) were detected separately. Group without symptoms and numbered Test, group, 10 strains into one group, the collected leaves are divided into two parts, which are used for enzyme-linked detection and molecular biological detection, respectively, less than 10 strains per plant Sampling for testing. 5.2 Detection method 5.2.1 Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) The prepared sample supernatant was added to a 96-well enzyme-linked plate coated with an ORSV antibody, and subjected to DAS-ELISA. Each sample ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 4077-2014_English be delivered?Answer: Upon your order, we will start to translate SN/T 4077-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically in 9 seconds (download/delivered in 9 seconds). The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 4077-2014_English with my colleagues?Answer: Yes. 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