SN/T 4067-2014 English PDF

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SN/T 4067-2014: Quarantine protocol for measles to entry-exit monkey
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4067-2014	289	Add to Cart	3 days	Quarantine protocol for measles to entry-exit monkey	Valid

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Basic data

Standard ID: SN/T 4067-2014 (SN/T4067-2014)
Description (Translated English): Quarantine protocol for measles to entry-exit monkey
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 11,144
Date of Issue: 12/1/2014
Date of Implementation: 5/1/2015
Quoted Standard: GB/T 6682; GB 19489; SN/T 2025
Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the monkey Isolation of measles virus, virus neutralization test, real-time PCR test, the technical requirements of blocking antibody ELISA test. This standard applies to the entry and exit monkey measles virus quarantine.

SN/T 4067-2014: Quarantine protocol for measles to entry-exit monkey

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for measles to entry-exit monkey People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Technical specification for quarantine of entry and exit monkey measles virus Released on.2014-11-19 2015-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Hainan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Guangdong Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Li Dandan, Xu Yigang, Ai Jun, Ji Xincheng, Xu Weijia, Lin Zhixiong, Wu Shannan, Huang Jihui. Technical specification for quarantine of entry and exit monkey measles virus

1 Scope

This standard specifies the pathogen isolation of monkey measles virus, virus neutralization test, real-time fluorescent quantitative PCR test, blocking ELISA antibody Technical requirements for the test. This standard applies to the quarantine of entry and exit monkey measles virus.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 General requirements for laboratory biosafety SN/T 2025 Animal Quarantine Laboratory Biosafety Practices

3 Abbreviations

The following abbreviations apply to this document. B95a cells. simian lymphoblasts CPE. cytopathic effect Ct value. the number of cycles experienced by the fluorescent signal reaching the set threshold (cyclethreshold) ELISA. enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay) MV. measles virus TCID50. half tissue culture infection (tissuecultureinfectivedose)

4 preliminary diagnosis

For the introduction of the disease, see A.1, the initial diagnosis can be based on clinical diagnosis and pathological changes, see A.2, A.3, and the laboratory is needed for diagnosis. diagnosis.

5 virus separation

5.1 Equipment and equipment Inverted optical microscope, carbon dioxide incubator, refrigerator (-20 ° C, -70 ° C), 50 μL, 100 μL micro-adjustable pipette, sterile 96-well cell culture plate, sterile plastic dripper, sterile plastic centrifuge tube (1.5mL) or 5mL~10mL sterile glass test tube, sterile cotton Swab. 5.2 Reagents and materials 5.2.1 Virus. The standard strain of MV virus. See the GB 19489 for the experimental environment and protection requirements. ......

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