SN/T 4002-2014 English PDF

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SN/T 4002-2014: Determination of silybin in health foods for export - HPLC method
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 4002-2014	369	Add to Cart	3 days	Determination of silybin in health foods for export - HPLC method	Valid

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Basic data

Standard ID: SN/T 4002-2014 (SN/T4002-2014)
Description (Translated English): Determination of silybin in health foods for export - HPLC method
Sector / Industry: Commodity Inspection Standard (Recommended)
Word Count Estimation: 14,161
Date of Issue: 12/1/2014
Date of Implementation: 5/1/2015
Quoted Standard: GB/T 6682
Regulation (derived from): State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies a high performance liquid chromatographic method silibinin health foods. This standard applies to capsules and tablets class DETERMINATION silybin care.

SN/T 4002-2014: Determination of silybin in health foods for export - HPLC method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Export health foods silibinin High performance liquid chromatography) People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of export health food in the silibinin High Performance Liquid Chromatography Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Hunan Exit Inspection and Quarantine, Inspection and Quarantine Institute of Science and Technology in Hunan Province, China Xiamen People's Republic of Exit Inspection and Quarantine. The main drafters of this standard. Mei-ling, Shiya Mei, Jiao Yanna, Liang Jingtao, Fushan Liang, Yanhong Fei, Zhang Ying, Huang Zhiqiang. Determination of export health food in the silibinin High Performance Liquid Chromatography

1 Scope

This standard specifies a high performance liquid chromatographic method silibinin health foods. This standard applies to capsules and tablets class DETERMINATION silybin care.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 Samples silibinin methanol ultrasonic extraction, HPLC, external standard.

4 Reagents and materials

Except where noted, all reagents were of analytical grade water as a water line with GB/T 6682 regulations. 4.1 Methanol. HPLC grade. 4.2 phosphoric acid. 4.3 sodium dihydrogen phosphate. 4.4 phosphoric acid solution (0.2mol/L). Pipette 1.33mL phosphate in 100mL volumetric flask, and dilute with water to volume. Sodium dihydrogen phosphate 4.5 (0.5mol/L). said take 6.0g of sodium dihydrogen phosphate in 100mL flask, dissolved in water and dilute to Scale. 4.6 silibinin Standards (CAS Number. 22888-70-6, molecular formula. C25H22O10). silibinin A and silibinin B mixture, pure Greater than equal to 98%. 4.7 silibinin standard stock solution. Weigh water silibinin standard (4.6) amount, dissolved in methanol to prepare a concentration of 1.0mg/mL of Standard stock solution, 4 ℃ stored in the dark. 4.8 silibinin standard solution. draw the right amount of silibinin standard stock solution (4.7), diluted to the appropriate concentration range mark with methanol Quasi working solution, preparation before use.

5 Instruments

5.1 HPLC - with diode array detector. 5.2 ultrasonic cleaner. 5.3 Analysis of balance. a sense of the amount of 0.0001g and 0.01g. ......

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