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SN/T 3949-2014 English PDF

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SN/T 3949-2014: Plastic package - Determination of harmful material bisphenol A - Conjuction of antigen and antibody method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 3949-2014209 Add to Cart 3 days Plastic package - Determination of harmful material bisphenol A - Conjuction of antigen and antibody method Valid

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GB/T 325.6   SN/T 4148   GB/T 6543   SN/T 5154   SN/T 3945   SN/T 3944   

Basic data

Standard ID: SN/T 3949-2014 (SN/T3949-2014)
Description (Translated English): Plastic package - Determination of harmful material bisphenol A - Conjuction of antigen and antibody method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: A82
Classification of International Standard: 55.040
Word Count Estimation: 8,879
Date of Issue: 4/9/2014
Date of Implementation: 11/1/2014
Quoted Standard: GB/T 6682
Regulation (derived from): State inspection certification (2014) 210
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the antigen-antibody plastic packaging bisphenol A binding assay method and reagent materials, instruments, test procedures and results of calculations. This standard applies to the determination of bisphenol A in plastic packaging

SN/T 3949-2014: Plastic package - Determination of harmful material bisphenol A - Conjuction of antigen and antibody method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Detection of plastic packaging of hazardous substances bisphenol A antigen-antibody binding method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Plastic packaging of hazardous substances detection of bisphenol A Antigen-antibody binding method Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Science and Technology Co., Ltd. inshore. The main drafters of this standard. Miao Wenbin Zhou Hui, Chen Xiang, Ran Xiaoyuan, Taohai Hua, ZHANG Xiaorong, Cai Lijun, Zhu Hongkun, Jiang Wei, Li Wei, Zheng Hua, Cai Xiaofeng Zhu of stars. Plastic packaging of hazardous substances detection of bisphenol A Antigen-antibody binding method

1 Scope

This standard specifies the antigen-antibody plastic packaging bisphenol A binding assay method and reagent materials, instruments, procedures and test results Calculations. This standard applies to the determination of bisphenol A in plastic packaging.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 PRINCIPLE OF THE METHOD

After ultrasonic plastic filtrate obtained after extraction for subsequent testing. Microtiter plates in a fixed amount of bisphenol A conjugate antigens, plus After the sample solution or a standard solution of bisphenol A, followed by the addition of bisphenol A certain amount of specific antibody, and the immobilized antigen so that the antigen competition With contention free in solution of bisphenol A specific antibody binding, thoroughly washed unreacted antibody and antigen, and then adding a certain amount of enzyme Secondary antibody (anti-antibody) amplifying effect, followed by sufficient washing, chromogenic substrate VIS color. As a result, the more the antigen content of the solid phase antibody The less bound antibody, the amount of enzyme-labeled secondary antibody, the less, the color the more shallow, negative correlation between the two. The conditions for this experiment are. fixed limit antigen, anti Quantitative body, anti-HRP II limits. The same site with the immobilized antigen and antibody binding to the antigen, the antibody binding competitively.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade. Test water should comply with GB/T 6682 in a water specifications. 4.1 Bisphenol A standard (HPLC grade, more than 99%). 4.2 methanol. 4.3 bovine serum albumin. 4.4 sodium bicarbonate. 4.5 sodium carbonate. 4.6 Tween 20. 4.7 disodium hydrogenphosphate dodecahydrate. 4.8 potassium dihydrogen phosphate. 4.9 potassium chloride. 4.10 sodium chloride. 4.11 citric acid. 4.12 sodium acetate. 4.13 glycerol.
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