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Plastic package - Determination of harmful material bisphenol A - Conjuction of antigen and antibody method
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Basic data
| Standard ID | SN/T 3949-2014 (SN/T3949-2014) |
| Description (Translated English) | Plastic package - Determination of harmful material bisphenol A - Conjuction of antigen and antibody method |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | A82 |
| Classification of International Standard | 55.040 |
| Word Count Estimation | 8,874 |
| Date of Issue | 4/9/2014 |
| Date of Implementation | 11/1/2014 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | State inspection certification (2014) 210 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the antigen-antibody plastic packaging bisphenol A binding assay method and reagent materials, instruments, test procedures and results of calculations. This standard applies to the determination of bisphenol A in plastic packaging |
SN/T 3949-2014: Plastic package - Determination of harmful material bisphenol A - Conjuction of antigen and antibody method
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(Detection of plastic packaging of hazardous substances bisphenol A antigen-antibody binding method)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Plastic packaging of hazardous substances detection of bisphenol A
Antigen-antibody binding method
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Science and Technology Co., Ltd. inshore.
The main drafters of this standard. Miao Wenbin Zhou Hui, Chen Xiang, Ran Xiaoyuan, Taohai Hua, ZHANG Xiaorong, Cai Lijun, Zhu Hongkun, Jiang Wei, Li Wei, Zheng Hua,
Cai Xiaofeng Zhu of stars.
Plastic packaging of hazardous substances detection of bisphenol A
Antigen-antibody binding method
1 Scope
This standard specifies the antigen-antibody plastic packaging bisphenol A binding assay method and reagent materials, instruments, procedures and test results
Calculations.
This standard applies to the determination of bisphenol A in plastic packaging.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 PRINCIPLE OF THE METHOD
After ultrasonic plastic filtrate obtained after extraction for subsequent testing. Microtiter plates in a fixed amount of bisphenol A conjugate antigens, plus
After the sample solution or a standard solution of bisphenol A, followed by the addition of bisphenol A certain amount of specific antibody, and the immobilized antigen so that the antigen competition
With contention free in solution of bisphenol A specific antibody binding, thoroughly washed unreacted antibody and antigen, and then adding a certain amount of enzyme
Secondary antibody (anti-antibody) amplifying effect, followed by sufficient washing, chromogenic substrate VIS color. As a result, the more the antigen content of the solid phase antibody
The less bound antibody, the amount of enzyme-labeled secondary antibody, the less, the color the more shallow, negative correlation between the two. The conditions for this experiment are. fixed limit antigen, anti
Quantitative body, anti-HRP II limits. The same site with the immobilized antigen and antibody binding to the antigen, the antibody binding competitively.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade. Test water should comply with GB/T 6682 in a water specifications.
4.1 Bisphenol A standard (HPLC grade, more than 99%).
4.2 methanol.
4.3 bovine serum albumin.
4.4 sodium bicarbonate.
4.5 sodium carbonate.
4.6 Tween 20.
4.7 disodium hydrogenphosphate dodecahydrate.
4.8 potassium dihydrogen phosphate.
4.9 potassium chloride.
4.10 sodium chloride.
4.11 citric acid.
4.12 sodium acetate.
4.13 glycerol.
...
