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SN/T 3895-2014 English PDF

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SN/T 3895-2014: Real-time fluorescence PCR method for detection of genetically modified components in triffid flax(Event FP967)
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 3895-2014319 Add to Cart 3 days Real-time fluorescence PCR method for detection of genetically modified components in triffid flax(Event FP967) Valid

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GB/T 19630   NY/T 1420   GB/T 19495.7   SN/T 4561   SN/T 3542   SN/T 3491   

Basic data

Standard ID: SN/T 3895-2014 (SN/T3895-2014)
Description (Translated English): Real-time fluorescence PCR method for detection of genetically modified components in triffid flax(Event FP967)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B04
Classification of International Standard: 65.020
Word Count Estimation: 12,138
Quoted Standard: GB/T 6682; GB/T 19495.2; GB/T 19495.3
Regulation (derived from): AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the transgenic linseed FP967 lines (also known as CDC Triffid) real-time PCR detection method. This standard applies to transgenic linseed FP967 lines (also known as CDC Triffid) qualitative detection.

SN/T 3895-2014: Real-time fluorescence PCR method for detection of genetically modified components in triffid flax(Event FP967)


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Flaxseed FP967 transgenic strains of real-time PCR detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Transgenic Linseed FP967 line real-time fluorescence PCR detection method componentsintriffidflax (EventFP967) Issued on. 2014-01-13 2014-08-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard reference of the EU-CRL "NOST-Specconstruct-specificmethodforthedetectionofCDCtriffid flax (EventFP967) usingreal-timePCR "method. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. Juan Cao occasion, Zhang Ying, Wang Jinling, Xu Junyi, Jia Jinsheng. Transgenic Linseed FP967 line real-time fluorescence PCR detection method

1 Scope

This standard specifies the transgenic linseed FP967 lines (also known as CDCTriffid) real-time PCR detection method. This standard applies to transgenic linseed FP967 lines (also referred to CDCTriffid) for qualitative detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 19495.3 GMO detection of nucleic acid extraction and purification method 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this document. 3.1.1 Transgenic transgene The species itself does not have derived from other species of functional DNA sequences, by introducing a variety of means to make it into the species Line expression, in order to obtain new varieties of the species characteristics. 3.1.2 Endogenous gene endogenousgene Species cultivated in copy number constant, allele variation is not displayed. The gene can be used for a purpose genome Analysis of the gene. 3.2 Acronyms The following abbreviations apply to this document. Ct values. C. Cycle, t. threshold, the number of cycles in each reaction tube fluorescent signal reaches the set threshold experienced. DNA. deoxyribonucleicacid, DNA. dNTP. deoxyribonucleosidetriphosphate, deoxynucleoside triphosphates. EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid. NOST-Spec. NOSterminatorandspectinomycinresistancegene, NOS terminator - spectinomycin resistance gene because. Spectinomycin resistance gene strains of transgenic linseed FP967 is a unique gene, it is not currently in any other genetically modified crops use. PCR. polymerasechainreaction, called PCR. SAD. stearoyl-acylcarrierproteindesaturase, stearoyl - acyl carrier protein desaturase. Tris. tris (hydroxymethyl) aminomethane, tris (hydroxymethyl) aminomethane.
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