SN/T 3579-2013 English PDFUS$159.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 3579-2013: Viability detection of Phytophthora sojae Kaufmann and Gerdemann Status: Valid
Basic dataStandard ID: SN/T 3579-2013 (SN/T3579-2013)Description (Translated English): Viability detection of Phytophthora sojae Kaufmann and Gerdemann Sector / Industry: Commodity Inspection Standard (Recommended) Word Count Estimation: 6,665 Regulation (derived from): ?AQSIQ About publishing 2013 First Batch 179-items Entry-Exit Inspection and Quarantine industry standard Announcement; Industry-Standard-Filing Announcement 2013 No. 9 (Total No. 165) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the application of laser scanning confocal microscope pathogen Phytophthora sojae (Phytophthora sojae Kaufmann & Gerdemann) activity detection method. This standard applies to carrying soybeans and soybean soil pathogen Phytophthor SN/T 3579-2013: Viability detection of Phytophthora sojae Kaufmann and Gerdemann---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Viability detection of Phytophthora sojae Kaufmann People's Republic of China Entry-Exit Inspection and Quarantine Standards Phytophthora pathogen activity detection method Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Structure does not bear the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, Guangdong micro Institute of Biology, Chinese Academy of Inspection and Quarantine. The main drafters. Zhang Guiming, Cheng Yinghui, Wang Ying, Chen Chi-nan, Zhong Jianzhong, Li Qiufeng, only to Yu, on behalf of the Beijing Lufthansa, Wang Ying, Chen Hongjun. Phytophthora pathogen activity detection method1 ScopeThis standard specifies the application of laser scanning confocal microscopy Phytophthora pathogen (PhytophthorasojaeKaufmann Gerdemann) method for detecting the activity. Assay This standard applies to soybeans and soil carried Phytophthora pathogen. Principle 2 Application of fluorescent dyes pathogen Phytophthora sojae oospores dyeing process, according to Phytophthora pathogen non-staining activity oospore After the egg plastid broken edge set, oval mass substantially uniform after the dense fluorescence and green are active oospore stained green dyeing effect difference, Using laser scanning confocal microscope to detect spores, analyze its activity situation. 3 appliances, instruments and reagents 3.1 Instrument appliances Laser scanning confocal microscope, microscopes, autoclaves, biological safety cabinet, incubator, refrigerator. 3.2 Reagents Set acridine orange, phosphate buffered saline (PBS, 0.1mol/μL, pH7.0), water agar medium.4 activity test method4.1 Get pathogen spores Under a microscope, the discovery and identification of the pathogen Phytophthora sojae oospores pick sterilized water to prepare a concentration of greater than 1 spore/μL of Spore suspension, microscopy, spare. 4.2 Spore staining Use the concentration of 100μg/mL (solvent PBS) acridine orange stain fixed with a spore suspension of a volume mixing ratio of 1.20 uniform Together, dark staining 15min at room temperature, 16000g supernatant was centrifuged 1min termination dyeing, sterilizing deionized water once, resuspended spore Spore concentration is greater than 1/μL. Dark for backup. 4.3 Laser scanning confocal microscope to detect activity 4.3.1 Sample preparation will be completed stained slides were mounted upside down on a laser scanning confocal microscope stage, low magnification to find the spores, Go to 63 times the oil microscope, to fine-tune the image within the field of vision clear. 4.3.2 Select the appropriate laser tube (argon ion laser) fluorescence excitation signal, setting an excitation wavelength of fluorescence channels (488nm), collect fluorescent The emission wavelength of the optical signal (505nm), and set a bright field channel as a control. 4.3.3 Choose low-resolution scanning plane rough scan (xy. 512 × 512), based on the scan imaging fluorescence signal strength ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 3579-2013_English be delivered?Answer: Upon your order, we will start to translate SN/T 3579-2013_English as soon as possible, and keep you informed of the progress. 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