SN/T 3567.8-2017 English PDF

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SN/T 3567.8-2017: Detection method of crossing priming isothermal amplification - Part 8: Plasmodium
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Similar standards

GBZ 57   GB/T 31989   SN/T 3567.11   SN/T 3567.7   SN/T 3567.10   

Basic data

Standard ID: SN/T 3567.8-2017 (SN/T3567.8-2017)
Description (Translated English): Detection method of crossing priming isothermal amplification - Part 8: Plasmodium
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C62
Classification of International Standard: 13.020
Word Count Estimation: 9,955
Date of Issue: 2017-07-21
Date of Implementation: 2018-03-01
Quoted Standard: GB/T 6682; GB 19489; SN/T 3562
Regulation (derived from): National Quality Inspection (2017) No. 337
Issuing agency(ies): General Administration of Customs
Summary: This standard stipulates the detection object, test procedure and test result report of Plasmodium detection by cross-primer constant temperature amplification method at border ports. This standard applies to the rapid screening and detection of Plasmodium at border ports.

SN/T 3567.8-2017: Detection method of crossing priming isothermal amplification - Part 8: Plasmodium

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method of crossing priming isothermal amplification - Part 8. Plasmodium People's Republic of China entry and exit inspection and quarantine industry standards Cross primer constant temperature amplification detection method Part 8. Plasmodium Part 8. Plasmodium Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 3567 "Cross Primer Constant Temperature Amplification Detection Method" is divided into 11 parts. --- Part 1. General technical procedures; --- Part 2. Vibrio cholerae; --- Part 3. E. coli O157.H7; --- Part 4. Yellow fever virus; --- Part 5. Salmonella; --- Part 6. Mycobacterium tuberculosis; --- Part 7. Enterovirus 71; --- Part 8. Plasmodium; --- Part 9. Yersinia pestis; ---Part 10. Bacillus anthracis; --- Part 11. Shigella. This part is the eighth part of SN/T 3567. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Tianjin International Travel Health Care Center, Hangzhou Yousida Biotechnology Co., Ltd. The main drafters of this section. Zuo Feng, Guan Yu, Zhao Xin, Qi Jun, Hu Lin, and especially Min. Cross primer constant temperature amplification detection method Part 8. Plasmodium

1 Scope

This part of SN/T 3567 specifies the detection targets and detection procedures for the detection of Plasmodium by cross-primer thermostat amplification at border ports. Sequence and test results report. This section applies to the rapid screening and detection of Plasmodium at border ports.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 General requirements for laboratory biosafety PCR detection method for Plasmodium SN/T 3562 border port List of pathogenic microorganisms transmitted from humans (Ministry of Health.2006)

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Cross-primer constant temperature amplification technology crossingprimingisothermalamplification; CPA A nucleic acid constant temperature amplification technology, in addition to DNA polymerase with DNA stranding function in the CPA constant temperature amplification system (DNAPoly- In addition to merase, it mainly includes cross-primers, stripping primers and detection primers. These oligonucleotide strands can rely on the DNA polymerase The highly active strand displacement property enables the cyclic amplification of deoxyribonucleic acid (DNA) to be continuously realized. 3.2 Cross primer primer Primary primer for cross-amplification, in which the 5' end sequence of the forward primer is identical to the hybridization sequence of the reverse primer, and the reverse primer The 5' end sequence is identical to the hybridization sequence of the forward primer, so the two primers are introduced into each other's hybridization sequence during amplification. Increase the hybridization site of the primer to promote the amplification reaction. 3.3 Stripping primer bumperprimer A short-stranded primer located behind the cross-amplification primer, which functions to extend the amplification primer under the action of a strand-replacement DNA polymerase The chain is peeled off from the template. 3.4 Detection primer detectionprimer A pair of short-stranded primers located inside the cross-primer, which need to be labeled with a hapten or fluorescein, so that the amplification product carries the target Remember to use for testing. ......

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