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Identification protocol for Trachidermus fasciatus. PCR
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Basic data
| Standard ID | SN/T 3483-2013 (SN/T3483-2013) |
| Description (Translated English) | Identification protocol for Trachidermus fasciatus. PCR |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B52 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 12,124 |
| Quoted Standard | GB/T 6682 |
| Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the technical specifications Songjiang perch species identification PCR methods. This standard applies to germplasm Songjiang perch. |
Similar standards
GB 5009.261|SN/T 5604|SN/T 3483-2013: Identification protocol for Trachidermus fasciatus. PCR
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Identification protocol for Trachidermus fasciatus. PCR
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Songjiang perch species identification methods
PCR methods
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong CIQ.
The main drafters of this standard. Xu Biao, Zhao Ran, Yue Zhiqin, Zhangtai Xiang, Zheng Xiaolong, Zhao Wei.
Songjiang perch species identification methods
PCR methods
1 Scope
This standard specifies the technical specifications Songjiang perch species identification PCR methods.
This standard applies to germplasm Songjiang perch.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Ct value of the cycle threshold
Inflection corresponding to the real-time PCR cycles when the fluorescent signal from the background into the exponential growth phase.
3.2
Amplification curve amplificationcurve
In real-time PCR reaction, the template is amplified, real-time PCR product was subjected to linear growth, exponential growth phase
And the platform of the three stages, the amount of real-time fluorescence quantitative PCR product changes with time and the curve obtained.
4 technical principles
This experiment was conserved and specific primers were designed to explore the use of Songjiang perch and cytochrome b (Cytochromeb, Cytb) gene
Needle, establish real-time PCR method. The method is based on the conventional PCR, to add a specific fluorescent probe. Probe
For the period of oligonucleotides, both ends of the report marks a fluorophore and a quencher fluorophore. The probe is intact, the reporter group launched
The fluorescent signal is quenched group absorption; real-time quantitative PCR amplification, Taq polymerase 5'-3 'exonuclease activity of the enzyme degradation of the probe, so that
Report fluorophore and quencher fluorophore separate, thus fluorescence monitoring system can receive the fluorescent signal, that is, each strand of DNA amplification,
There is a fluorescent molecule is formed to achieve the formation and accumulation of PCR product fluorescent signal completely synchronized. This method has the real-time, quantitative,
Specificity, high sensitivity advantages.
5 Reagents and materials
5.1 experimental water
Shall comply with GB/T 6682's.
...
