SN/T 3483-2013 English PDF

US$319.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 3483-2013: Identification protocol for Trachidermus fasciatus. PCR
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 3483-2013	319	Add to Cart	3 days	Identification protocol for Trachidermus fasciatus. PCR	Valid

Similar standards

GB 5009.261   SN/T 5604   SN/T 5487   SN/T 2699   

Basic data

Standard ID: SN/T 3483-2013 (SN/T3483-2013)
Description (Translated English): Identification protocol for Trachidermus fasciatus. PCR
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B52
Classification of International Standard: 07.080
Word Count Estimation: 12,162
Quoted Standard: GB/T 6682
Regulation (derived from): AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the technical specifications Songjiang perch species identification PCR methods. This standard applies to germplasm Songjiang perch.

SN/T 3483-2013: Identification protocol for Trachidermus fasciatus. PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification protocol for Trachidermus fasciatus. PCR People's Republic of China Entry-Exit Inspection and Quarantine Standards Songjiang perch species identification methods PCR methods Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shandong CIQ. The main drafters of this standard. Xu Biao, Zhao Ran, Yue Zhiqin, Zhangtai Xiang, Zheng Xiaolong, Zhao Wei. Songjiang perch species identification methods PCR methods

1 Scope

This standard specifies the technical specifications Songjiang perch species identification PCR methods. This standard applies to germplasm Songjiang perch.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Ct value of the cycle threshold Inflection corresponding to the real-time PCR cycles when the fluorescent signal from the background into the exponential growth phase. 3.2 Amplification curve amplificationcurve In real-time PCR reaction, the template is amplified, real-time PCR product was subjected to linear growth, exponential growth phase And the platform of the three stages, the amount of real-time fluorescence quantitative PCR product changes with time and the curve obtained.

4 technical principles

This experiment was conserved and specific primers were designed to explore the use of Songjiang perch and cytochrome b (Cytochromeb, Cytb) gene Needle, establish real-time PCR method. The method is based on the conventional PCR, to add a specific fluorescent probe. Probe For the period of oligonucleotides, both ends of the report marks a fluorophore and a quencher fluorophore. The probe is intact, the reporter group launched The fluorescent signal is quenched group absorption; real-time quantitative PCR amplification, Taq polymerase 5'-3 'exonuclease activity of the enzyme degradation of the probe, so that Report fluorophore and quencher fluorophore separate, thus fluorescence monitoring system can receive the fluorescent signal, that is, each strand of DNA amplification, There is a fluorescent molecule is formed to achieve the formation and accumulation of PCR product fluorescent signal completely synchronized. This method has the real-time, quantitative, Specificity, high sensitivity advantages.

5 Reagents and materials

5.1 experimental water Shall comply with GB/T 6682's. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 3483-2013_English be delivered?

Answer: Upon your order, we will start to translate SN/T 3483-2013_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 3483-2013_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 3483-2013_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.