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SN/T 3380-2012 English PDF

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SN/T 3380-2012: Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 3380-2012209 Add to Cart 3 days Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method Valid

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Basic data

Standard ID: SN/T 3380-2012 (SN/T3380-2012)
Description (Translated English): Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method
Sector / Industry: Commodity Inspection Standard (Recommended)
Word Count Estimation: 8,881
Date of Issue: 12/12/2012
Date of Implementation: 7/1/2013
Regulation (derived from): State-Quality-Inspection-accreditation (2012) 777
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the method of enzyme-linked immunoassay four nitrofuran metabolite residues in food of animal origin exports. This standard applies to chicken, pork, crayfish, back to the fish, milk, honey and other food of animal origin furazolid

SN/T 3380-2012: Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export.ELISA method People's Republic of China Entry-Exit Inspection and Quarantine Standards Export of food of animal origin nitrofuran metabolites Determination of the residual ELISA Issued on. 2012-12-12 2013-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, Changzhou, People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Shandong Entry-Exit Inspection and Quarantine Yancheng People's Republic of China. The main drafters of this standard. Yu Bing, Cai Baoliang, Li Dongming, Yuan Tao, Yuan Fang, Xu Xing help, DingTao. Export of food of animal origin nitrofuran metabolites Determination of the residual ELISA

1 Scope

This standard specifies four methods of enzyme-linked immunosorbent assay nitrofuran metabolites residues in food of animal origin exports. This standard applies to chicken, pork, crawfish, back to the fish, milk, honey and other food of animal origin furazolidone metabolite (AOZ), furan It is one metabolite (AMOZ), nitrofurazone metabolite (SEM), nitrofurantoin metabolite (AHD) residues detected. Principle 2 Determination of the basis of this method is a competitive enzyme immunoassay, the overall process includes four nitrofuran metabolites residues detected. in Microtiter plates pre-coated strips and conjugate antigen in the sample nitrofuran metabolites residues after derivatized and even pre-coated on the strips are of Associated antigen antibody anti-competitive corresponding derivatives of nitrofuran metabolites after adding enzyme-labeled secondary antibody with TMB chromogenic substrate, the sample absorbance value Its content contained nitrofuran metabolites residues negatively correlated with the standard curve and multiplied by the corresponding dilution factor can be derived sample The remaining amount of nitrofuran metabolites residues.

3 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade, water is deionized water. 3.1 methanol. 3.2 ethyl acetate. 3.3 n-hexane. 3.4 acetonitrile. 3.5 of concentrated hydrochloric acid. 3.6 sodium hydroxide. 3.7 dipotassium hydrogen phosphate. 3.8 nitroso ferricyanide. 3.9 zinc sulfate. 3.10 pairs carboxybenzaldehyde. 3.11 1mol/L hydrochloric acid (HCl). Take 8.6mL of concentrated HCl and add water to dissolve 100mL. 3.12 0.1mol/L potassium phosphate dibasic (K2HPO4). said 22.8gK2HPO4 · 3H2O dissolved with deionized water solution to 1L. 3.13 nitroso ferricyanide (K2Fe (CN) 5 · NO · 3H2O) solution. 12.5g nitroso ferricyanide deionized water to dissolve 100mL. 3.14 zinc sulfate (ZnSO4 · 7H2O) solution. 29.8g zinc sulfate dissolved with deionized water to dissolve 100mL. 3.15 derivatization reagent (0.01mol/L CARBOXYBENZALDEHYDE solution). 15.013mg CARBOXYBENZALDEHYDE dissolved in methanol and set the volume to 10mL. 3.16 Standard. includes AOZ, AMOZ, SEM and AHD four standards, the purity of ≥99%. Preparation of standard solution of 3.17. were accurately weighed amount of AOZ, AMOZ, SEM and AHD four standards, paired with acetonitrile 1mg/mL standard stock solution at 4 ℃ ~ 8 ℃ preservation conditions.
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