SN/T 3330-2012 English PDFUS$239.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 3330-2012: Protocol of identification for Tachypleus tridentatus. PCR method Status: Valid
Basic dataStandard ID: SN/T 3330-2012 (SN/T3330-2012)Description (Translated English): Protocol of identification for Tachypleus tridentatus. PCR method Sector / Industry: Commodity Inspection Standard (Recommended) Word Count Estimation: 9,995 Quoted Standard: GB/T 6682; SN/T 1193 Regulation (derived from): National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the Chinese horseshoe crab species identified by PCR method. This standard applies to the entry and exit aquatic products and ingredients containing Chinese horseshoe crab biological agents in the identification of Chinese horsesho SN/T 3330-2012: Protocol of identification for Tachypleus tridentatus. PCR method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Protocol of identification for Tachypleus tridentatus.PCR method People's Republic of China Entry-Exit Inspection and Quarantine Standards Chinese horseshoe crab species identification by PCR methods Issued on. 2012-12-12 2013-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Structure does not bear the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Yantai Exit Inspection and Quarantine, People's Republic of China Shandong Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Yin Wei force, Fangshao Qing, Wang Ying, Yue Zhiqin, Xu Hongyan, Liu Ning, bell, Lu Min, Duan Hui efficiency, Luan Jing, Zhang Jingxuan, Su Zhi Ping, Gengjin Pei. Chinese horseshoe crab species identification by PCR methods1 ScopeThis standard specifies the Chinese horseshoe crab species by PCR method. This standard applies to entry and exit aquatic and biological preparations containing Chinese horseshoe crab ingredient identification of Chinese horseshoe crab.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis SN/T 1193 genetic testing laboratory technical requirements3 Terms and DefinitionsThe following terms and definitions apply to this document. 3.1 Polymerase chain reaction polymernasechainreaction; PCR In thermostable DNA polymerase and a pair of primers (tested with target nucleic acid molecule sequences homologous DNA fragments) at high temperature (DNA points Sub-denatured) and low temperature (primer profit target nucleic acid molecule complex nature and thermostable DNA polymerase extension) alternating cycles of amplification of the target nucleic acid test Child approach. Principle 4 Chinese horseshoe crab (morphological characteristics see Appendix A) sample after extraction of total genomic DNA, the use of specific primers designed by conventional PCR specific amplification technology Chinese horseshoe crab mtDNACOX3 gene fragment (349bp). Chinese horseshoe crab samples is determined according to the results of PCR amplification Is Chinese horseshoe crab species. Specific primers by mtDNA than Chinese horseshoe crab, Limulus round tail, American horseshoe crab, Limulus and other mostly southern horseshoe crab COX3 gene fragments designed.5 Equipment and Reagents5.1 Equipment PCR, tissue grinder, clean table, ice maker, high-speed refrigerated centrifuge, water bath, small desktop centrifuge, a conventional refrigerator, Vortex, electrophoresis, gel imaging system. 5.2 Reagents Unless otherwise specified, all reagents were of analytical grade. 5.2.1 Water. should be consistent with GB/T 6682 in a water specifications. For PCR (including nucleic acid extraction) are used when water with DEPC at Treated to remove RNA enzyme. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 3330-2012_English be delivered?Answer: Upon your order, we will start to translate SN/T 3330-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 3330-2012_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 3330-2012_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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