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SN/T 3329-2012 English PDF

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SN/T 3329-2012: Protocol of species identification for Equus przewalsrii. PCR method
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 3329-2012239 Add to Cart 3 days Protocol of species identification for Equus przewalsrii. PCR method Valid

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SN/T 5268   

Basic data

Standard ID: SN/T 3329-2012 (SN/T3329-2012)
Description (Translated English): Protocol of species identification for Equus przewalsrii. PCR method
Sector / Industry: Commodity Inspection Standard (Recommended)
Word Count Estimation: 9,967
Quoted Standard: GB/T 6682; GB 19489
Regulation (derived from): National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the Chinese Przewalski species identification by PCR method. This standard applies to China Przewalski species identification.

SN/T 3329-2012: Protocol of species identification for Equus przewalsrii. PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of species identification for Equus przewalsrii.PCR method People's Republic of China Entry-Exit Inspection and Quarantine Standards Species identification of Chinese Platts Broncos PCR methods PCRmethod Issued on. 2012-12-12 2013-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shandong Entry-Exit-Entry Inspection and Xinjiang People's Republic of China Quarantine Bureau. The main drafters of this standard. Zheng Xiaolong, Wang Qun, into the new season, Liang beads, China Zhu, Deng Mingjun, Yue Zhiqin, Xiao Xi Zhi, Xu Biao. Species identification of Chinese Platts Broncos PCR methods

1 Scope

This standard specifies the China Platts Mustang species identification by PCR method. This standard applies to species identification China Platts Mustang.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety

3 PRINCIPLE OF THE METHOD

According E.przewalskii mitochondrial DNA control region (D-Loop region) was designed and synthesized a pair of specific primers of the target gene E.przewalskii PCR amplification was performed to determine whether the sample contains a gene E.przewalskii. E.przewalskii morphology see Appendix A.

4 Equipment

4.1 PCR instrument. 4.2 high-speed desktop centrifuge. 4.3 desktop centrifuge. 4.4 Mixer. 4.5 refrigerator. 4.6 micro-adjustable pipette (10μL, 100μL, 1000μL) and supporting tip. 4.7 centrifuge tube.

5 Reagents and materials

5.1 ethanol. 5.2 distilled water (should be consistent with GB/T 6682 in a water specifications). 5.3 proteinase K. 5.4 sodium dodecyl sulfate (SDS). 5.5 cetyltrimethylammonium bromide (CTAB). 5.6 sodium chloride. 5.7 chloroform. 5.8 isoamyl alcohol.
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