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SN/T 3327-2012 English PDF

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US$209.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 3327-2012: Quarantine protocal for classic swine fever virus by loop-mediated isothermal amplification
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 3327-2012	209	Add to Cart	3 days	Quarantine protocal for classic swine fever virus by loop-mediated isothermal amplification	Valid

Similar standards

SN/T 5268

Basic data

Standard ID: SN/T 3327-2012 (SN/T3327-2012)
Description (Translated English): Quarantine protocal for classic swine fever virus by loop-mediated isothermal amplification
Sector / Industry: Commodity Inspection Standard (Recommended)
Word Count Estimation: 8,839
Quoted Standard: GB/T 18088; GB 19489-2008
Regulation (derived from): National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the method of operation of classical swine fever virus reverse transcriptase loop-mediated isothermal nucleic acid amplification testing. This standard applies to rapid screening of classical swine fever virus.

SN/T 3327-2012: Quarantine protocal for classic swine fever virus by loop-mediated isothermal amplification

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocal for classic swine fever virus by loop-mediated isothermal amplification People's Republic of China Entry-Exit Inspection and Quarantine Standards Classical swine fever virus by reverse transcription loop-mediated isothermal Nucleic acid amplification tests Quarantineprotocalforclassicswinefevervirusbyloop-I mediated Issued on. 2012-12-12 2013-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Li Jian, Xiong Wei, Zhang Qiang, Jiang Jing, Qiu Lu, Wang Qiao whole, Huang Zhongrong, Hu Yongqiang. Classical swine fever virus by reverse transcription loop-mediated isothermal Nucleic acid amplification tests

1 Scope

This standard specifies the method of operation of classical swine fever virus by reverse transcription loop-mediated isothermal nucleic acid amplification detection. This standard applies to rapid screening of classical swine fever virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 18088 Entry and Exit Animal Quarantine sampling GB 19489-2008 General requirements for laboratory biosafety

3 Overview

Classical swine fever (classicalswinefever, CSF), is a kind of emergency by the classical swine fever virus (classicalswinefevervirus, CSFV) caused Resistance, heat resistance, highly contagious disease of swine breeding industry very serious harm. CSFV genotype positive strand RNA, genome about 123kb, contains only one large open reading frame (ORF). The standard use of loop-mediated isothermal nucleic acid amplification at a constant temperature Fast, efficient and specifically amplify located fever virus 5 'untranslated region (5'-UTR) of the target sequence, so as to achieve rapid detection purposes.

4 Reagents and materials

4.1 BstDNA polymerase (large fragment). enzyme concentration was 8U/μL, -20 ℃, avoid repeated freezing and thawing. 4.2 10 times ThermoPol polymerase buffer concentrate. -20 ℃ to save. 4.3 magnesium sulfate (MgSO4). concentration of 25mmol/L, -20 ℃ preservation. 4.4 AMV reverse transcriptase. reverse transcriptase concentration of 5U/μL, -20 ℃, avoid repeated freezing and thawing. 4.5 betaine solution (Betaine). concentration 5mol/L, -20 ℃, avoid repeated freezing and thawing. 4.6 dNTPs. containing dATP, dGTP, dCTP, dTTP each 10mmol/L, -20 ℃ preservation. 4.7 LAMP primer. primer dubbed the concentration of 25μmol/L, -20 ℃ stored for use. Its sequence is as follows. --- Being outwardly primer (CSFV-F3). 5'-AGC-TCC-CTG-GGT-GGT-CTA-3 '; --- Reverse outer primer (CSFV-B3). 5'-CCT-AAT-AGT-GGG-CCT-CTG-CA-3 '; --- Within the forward primer (CSFV-FIP). 5'-GCC-CTC-GTC-CAC-ATA-GCA-TCT-CAG-TAC-AGG-ACA- GTC-GTC-AGT-3 '; --- Within the reverse primer (CSFV-BIP). 5'-GCC-CAA-GAC-ACA-CCT-TAA-CCC-TTC-AGG-TCG-TAC- TCC-CAT-CAC-3 '; --- Forward loop primer (CSFV-LF). 5'-GGG-CTT-CTG-CTC-ACG-TCG-3 '; --- Reverse loop primer (CSFV-LB). 5'-GGT-CGC-TAG-GGT-GAA-ATC-3 '. 4.8 SYBRGreenⅠ fluorescent dye solution. -20 ℃ to save. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 3327-2012_English be delivered?

Answer: Upon your order, we will start to translate SN/T 3327-2012_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 3327-2012_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 3327-2012_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.