SN/T 3027-2011 English PDFUS$389.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 3027-2011: Determination of fluoroquinolones residues in royal jelly for export. Enzyme-linked immunosorbent assay Status: Valid
Basic dataStandard ID: SN/T 3027-2011 (SN/T3027-2011)Description (Translated English): Determination of fluoroquinolones residues in royal jelly for export. Enzyme-linked immunosorbent assay Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B47 Classification of International Standard: 65.140 Word Count Estimation: 15,171 Date of Issue: 2011-09-09 Date of Implementation: 2012-04-01 Quoted Standard: GB/T 6682 Regulation (derived from): National-Quality-Inspection-accreditation (2011) 492 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the method of enzyme-linked immunoassay royal jelly Buddha quinolone residues. This standard applies to royal jelly nadifloxacin, rufloxacin, Danofloxacin, enrofloxacin, ciprofloxacin, levofloxacin, ofloxacin, Pefloxacin, norfloxacin, two Sarafloxacin determination and total residual enoxacin, difloxacin. SN/T 3027-2011: Determination of fluoroquinolones residues in royal jelly for export. Enzyme-linked immunosorbent assay---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Determination of fluoroquinolones residues in royal jelly for export.Enzyme-linked immunosorbent assay People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of fluoroquinolone residues in royal jelly export ELISA Issued on. 2011-09-09 2012-04-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordIn accordance with this standard GB/T 1.1-2009 rules drafted. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine. The main drafters. Zhang Xiaofeng, Zhu Zhenjiang, Wu Shan, Shuai Jiang Bing, Chen Xiaomei. Determination of fluoroquinolone residues in royal jelly export ELISA1 ScopeThis standard specifies the method of enzyme-linked immunosorbent assay Fluoroquinolones Residues in Royal Jelly. This standard applies to royal jelly nadifloxacin, rufloxacin, danofloxacin, enrofloxacin, ciprofloxacin, levofloxacin, oxyfluoride Gatifloxacin, measured pefloxacin, norfloxacin, difloxacin, enoxacin sarafloxacin and residual total.2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis Principle 3 Perchloric acid to precipitate proteins of royal jelly and royal jelly extract residues fluoroquinolones. Microplate is coated with a fluorine Connaught sand Star antibody binding horseradish peroxidase was added norfloxacin (norfloxacin enzyme-labeled), norfloxacin after standard or sample extract, standard Norfloxacin solution or sample and enzyme-labeled fluoroquinolones norfloxacin norfloxacin competitively bind to the antibody. By washing Remove unbound reagent, then adding chromogenic substrate, absorbance was measured using a microplate reader, the absorbance of the sample is worth fluoroquinolones content.4 Reagents and materialsUnless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations. Fluoroquinolone 4.1 Randox test kits 1). 1) This information is given for the convenience of users of this standard does not imply endorsement of a particular product. If other product has the same effect, subject to real Using these products is equivalent to a posteriori assessment. 4.2 norfloxacin enzyme conjugate solution. according to kit instructions fluoroquinolones, dilution with enzyme labels (see Appendix A) the fluorine Connaught Shaxing enzyme conjugate concentrate (see Appendix A) was dissolved, mix and reserve. Dilution 4.3 wash buffer. fluoroquinolones kit according to instructions, the diluted wash buffer concentrate (see Appendix A) Add 970mL diluted with water, after mixing spare. 4.4 phosphoric acid. AR. 4.5 NaCl. AR. 4.6 methanol. AR. 4.7 30% sodium hydroxide. 30gNaOH dissolved in water, water volume to 100mL. 4.8 6% perchloric acid. 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