SN/T 2853-2011 English PDF

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SN/T 2853-2011: Quarantine protocol for mikrocytos mackini
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2853-2011	709	Add to Cart	5 days	Quarantine protocol for mikrocytos mackini	Valid

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Basic data

Standard ID: SN/T 2853-2011 (SN/T2853-2011)
Description (Translated English): Quarantine protocol for mikrocytos mackini
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B51
Classification of International Standard: 65.150
Word Count Estimation: 27,262
Date of Issue: 2011-05-31
Date of Implementation: 2011-12-01
Quoted Standard: GB/T 6682
Regulation (derived from): ?AQSIQ-Inspection [2011] 291
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the clinical diagnostic methods for the detection of oyster closed cryptosporidiosis, organization blotting, tissue sectioning, transmission electron microscopy, PCR and in situ hybridization method and other methods. This standard applies to closed epidemiology of cryptosporidiosis, monitoring, diagnosis and entry-exit inspection and quarantine.

SN/T 2853-2011: Quarantine protocol for mikrocytos mackini

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for mikrocytos mackini People's Republic of China Entry-Exit Inspection and Quarantine Standards Closed cryptosporidiosis quarantine Technical Specifications Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is a reference to the use of the OIE "aquatic animal disease diagnostic manual" (2006 edition) in chapter 2.2.5 "Closed Infected" (in- fectionwithmikrocytosmackini), on the part concerning histology, transmission electron microscopy and in situ hybridization were complementary and perfect. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Xiamen Entry-Exit Inspection and Quarantine, Shenzhen People's Republic of China And the People's Republic of China Shandong CIQ. The main drafters of this standard. German Fan, Xu Shu Fei, Liu Orientin, Chen Xinzhong, Gong Yanqing, Wang Jingming, Zhoubin Hua, Xu Biao. Closed cryptosporidiosis quarantine Technical Specifications

1 Scope

This standard specifies the oyster closed for detecting clinical diagnosis of cryptosporidiosis, blotting tissue, tissue slice method, transmission electron microscopy, PCR method and in situ hybridization methods. This standard applies to closed oyster cryptosporidiosis epidemiological investigation, monitoring, diagnosis and exit inspection and quarantine.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 materials and equipment

3.1 Reagents and materials Unless otherwise specified, the following biochemical reagents were of analytical grade. Reagent preparation see Appendix A. Experimental water accord GB/T 6682 requirements. 3.1.1 Preparation of tissue sections and staining reagents. Giemsa (Giemsa) dye, hematoxylin - eosin staining solution, eosin dye, Bismarck brown Y, Back blue liquid. BCIP/NBT; Davidson's solution, formaldehyde, glutaraldehyde, amino alkyl silane coated slides, cover slips, paraffin, Epson 812, DNP-30, DDSA (dodecyl succinic anhydride), MNA (Rokko anhydride). 3.1.2 DNA extraction kit. proteinase K, phenol - chloroform extraction method required related reagents or other DNA extraction kit. 3.1.3 PCR Reagents. 10 × PCR buffer (not containing magnesium ions), 25mmol/L magnesium chloride, 10mmol/LdNTPs, 5U/μL HotstartTaq polymerase, DNAMakerDL2000,6 × electrophoresis sample buffer, and so on, are commercially available kit components. Agar Sugar 5 × electrophoresis buffer (TBE). 3.1.4 Organic reagents. methanol, ethanol, acetone, glycerol, isopropanol, xylene, propylene oxide, and nitrogen were mounted gums. 3.1.5 Inorganic Reagents. sodium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium azide, uranyl acetate and lead citrate seawater. 3.1.6 primers and probes. includes a pair of PCR primers and nucleic acid probe labeled with digoxin, is based on the gene sequence SSUrDNA (GenBank. AF477623. insect the size of a sequence of 1457bp fragment) design. Upstream primer. 5'-AGATGGTTAATGAGCCTCC-3 '(19bp, positions 318 - 336) Downstream primer. 5'-GCGAGGTGCCACAAGGC-3 '(17bp, positions 847 - 863) The primers amplified product size 546bp. Probe. 3 'end of the probe sequences land digoxin labeled sequence 5'-AGCCCACAGCCTTCAC-3'- digoxin (16bp, location 1287 ~ 1302). 3.1.7 negative and positive controls. designated by the State Administration of Quality Supervision, Inspection and Quarantine laboratory. 3.2 Equipment 3.2.1 slicer. 3.2.2 tissue grinder. ......

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