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Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2471-2019: (Quarantine and identification method of larch seed wasp and Pistacia chinensis seed bee) Status: Valid SN/T 2471: Historical versions
Basic dataStandard ID: SN/T 2471-2019 (SN/T2471-2019)Description (Translated English): (Quarantine and identification method of larch seed wasp and Pistacia chinensis seed bee) Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Word Count Estimation: 11,176 Date of Issue: 2019-09-03 Date of Implementation: 2020-03-01 Older Standard (superseded by this standard): SN/T 2471-2010 Regulation (derived from): Natural Resources Department Announcement No. 7 of 2019 Issuing agency(ies): General Administration of Customs SN/T 2471-2019: (Quarantine and identification method of larch seed wasp and Pistacia chinensis seed bee)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. (Quarantine and identification method of larch seed wasp and Pistacia chinensis seed bee) ICS 65.002.01B16 People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard SN/T 2471-2019 replaces Sn/T 2471-2010 Bumblebee and Pistacia chinensis Seed bee quarantine identification method Published.2019-2009-03 2020-03-01 implementation Published by the General Administration of Customs of the People's Republic of China ????? ????, ????, ?? ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 2471-2010. The main differences between this standard and SN/T 2471-2010. --- Adjusted the standard structure and content; --- Revised the biological information, geographical distribution and host plants of the two bees; --- Added the search form for common domestic similar types; --- Added the identification feature map of two small bees. This standard is proposed and managed by the General Administration of Customs of the People's Republic of China. This standard was drafted. China Academy of Inspection and Quarantine. The main drafters of this standard. Chen Yan, Jiang Fan, Huang Ying, Chen Naizhong. The previous versions of the standards replaced by this standard are. --- SN/T 2471-2010. SN/T 2471-201919 ????? ????, ????, ?? Bumblebee and Pistacia chinensis Seed bee quarantine identification method1 ScopeThis standard applies to the quarantine and identification of Larch seed bee and Pistacia seed bee.2 Terms and definitionsThe following terms and definitions apply to this document. 2.1 There are two grooves on both sides of the front edge of the mid-thoracic shield. 2.2 2.3 marginalvein). 2.4 2.53 Basic informationThere are 61 species of 11 genera in China, and 38 species of broad-shouldered genus in China. See Appendix A for the biology, geographic distribution, and host information of the two bees. Common species in China are the broad-shouldered bee, the slime beetle, the astragalus seed bee and the Chinese locust seed bee. Record B. SN/T 2471-201919 ????? ????, ????, ??4 Method principleThe classification and identification of small bees are mainly based on the external morphological characteristics of female adults. Larvae need to be reared to adulthood for identification.5 Equipment and reagents5.1 Equipment Stereo microscope, forceps, dissecting scissors, dissecting needle, petri dish, absorbent paper, small writing brush, finger tube. 5.2 Reagents Anhydrous ethanol.6 detection6.1 Larch seed bee 6.1.1 Grain inspection The extracted seed samples were dissected one by one (100 seeds). The specific operation method is as follows. first use a surgical shear to cut a small Mouth, then use an insect needle to gradually peel off the seeds, put the larva or pupae in a petri dish, and place it under a dissecting mirror, according to the morphological characteristics of the larva or pupae Preliminary identification. 6.1.2 Specific gravity test This method is usually used when the seed-containing rate is low. The specific operation method is as follows. 250g) put in clean water, the volume ratio of seeds to clean water is 1. 5, stir for 1min ~ 2min, leave it for 8h ~ 12h, and remove the upper floating seeds The seeds are dissected one by one, which can improve the efficiency of slicing examination. 6.2 Bumblebee seed bee 6.2.1 Visual inspection Place the extracted fruit samples on white paper or white porcelain plate for careful observation. The healthy and plump fruits are blue-green, and the injured fruits are red-brown. Color, easy to distinguish. Victim fruit is easily confused with immature or damp moldy fruit. immature fruit is smaller in size and has a thin peel, Yellow; and moist and moldy fruits are gray-black or gray-brown. 6.2.2 Grain inspection This method can be performed on the basis of visual inspection. The specific operation method is as follows. Put the sampled fruit in a white porcelain plate and change the color The fruit is picked out, crushed piece by piece with pliers, and gradually peeled off with a dissecting needle. To avoid crushing the fruit and larva Gauze is wrapped around the axis to ensure that the fruit shell is cracked without damaging the integrity of the insect body. 6.2.3 Specific gravity test This method is usually used when the fruit has a low insect content rate. The specific operation method is as follows. Put the extracted fruit sample into clear water, and the fruit The volume ratio of solid and clean water is 1. 5. After fully stirring, let stand for 30 minutes, and take out the upper floating fruit to slice and inspect, which can improve the inspection efficiency. SN/T 2471-201919 ????? ????, ????, ??7 Laboratory identification7.1 Identification Characteristics of Adults The body is medium in size, with a body length of 4mm-5mm, androgynous or heterotypic. The body is mostly black, sometimes yellow or yellowish, without metallic luster. Phytophagous (feeds on seeds, grasses stems) or parasitic. The head and chest often have deep umbilical nicks or are wrinkled. Frontal view of head wide and wide The jaw is strong, with 3 teeth, and the cheek is long. The tentacles are born in the middle of the face, 11 to 13 verses. The pronotum is rectangular, and the mid-thoracic shield sulcus is deep and complete; and the thoracoabdominal section is often wrinkled and obvious. The hind belly is smooth or smooth. The female's stalk has an obovate, oblate, laterally flattened end, with a plow-like or pillar-shaped upturn. Like, ovipositor slightly protruding. The male stalk has an abdomen with a long abdomen. Hind tibia with 2 pitches. 7.2 Identification Characteristics of Adults The anterior wing margin vein is obviously longer than the mole vein, and the male antennae cords are usually 5 knots. Carnivorous or herbivorous. Female. The frontal width of the head is slightly larger than the length, and the lower end is slightly narrow. The cheeks are equal to the compound eyes in length, and the face is often sunken. The tentacles are born in the middle of the face. The knots are linear, mostly 5 knots, and 3 knots. The chest is quite long, the back is bulging, and the anterior thoracic shield is 2-3 times wider than the long, slightly shorter than the mid-thoracic shield; Swelling, oval. And the thoracoabdominal section is significantly inclined, with large reticular wrinkles, and the middle and longitudinal grooves are narrow and deep. The front wings are transparent and the marginal veins are longer than the mole veins. Stalk abdomen Oval-shaped, several as long as the middle body, slightly flattened and pointed at the tip, the dorsal plate of the ventral end of the abdomen is shaped like a plow, and the ovipositor is slightly protruding. Male. Rounded posterior belly, with long belly. There are 5 tentacle cord sections, which are banana-shaped with stalks, and are connected to each other. Each section has hair growth, and 2 stick sections. The head and chest surface have large umbilical marks. 7.3 Identification of Larvae in Larch Seeds 7.3.1 Female adult Body length 1.8mm ~ 2.5mm. Body black without metallic luster. Compound eyes are brown. In addition to the foot and the body of the same color, the leg and tibia more or less black-brown to yellow-brown, tarsal joint yellow-brown, dark brown at the end. The wings are transparent and the veins are brown. Hind belly slightly reddish-brown, ends (including Ovipositor sheath) red dark brown. See Appendix C. The frontal view of the head is broad, and the face is slightly bulged except for the open antennae depressions, which are slightly swollen, and have silver-gray feathery setae. Compound eyes Large, smooth and hairless. The antennae are born in the middle of the face, above the line connecting the lower edges of the compound eyes. Antennae stalks are columnar, long and do not reach the top of the head; The knots are all longer than wide, and the first knot is the longest (1.5 times the width), and the subsequent knots gradually become wider; the stick knot is 3 knots. The longitudinal sulcus of the midthoracic shield is intact; the scutellum bulges and is oval; and the thoracoabdominal section is steeply oblique; the central depression is slightly shallow longitudinal groove; wrinkle. The anterior wing vein is about 2 times longer than the mole vein and 1.5 times longer than the trailing edge vein. The end of the mole vein is enlarged like a bird's head. And near the valves on both sides of the thorax and abdomen The setae on the anterior side of the hind foot are thicker and denser, and the dorsal side of the hind leg tibial section has thicker silver-gray bristles. Hairs line up. The abdomen is flat behind the stalk, longer than the head and chest combined. The back of the stigma is the longest in the fourth section, and the first to fourth sections are smoother. The last few knots together form a slightly upturned plow-like protrusion. 7.3.2 Male adult It is shorter than the female (body length 1.2mm ～ 2.2mm), and is different from the female. the antennal stalk section is enlarged, the cable section is also swollen and connected to the stalk-like shape, and the whip node has long hairs; Shaped, with a long belly. 7.3.3 Egg Milky white, oblong, about 0.1mm long, with filamentous ovules, white, slightly longer than the long diameter of the egg. 7.3.4 Larva White, aging yellowish, body length 2mm ~ 3mm, curved "C", no feet, very small head, developed upper jaw, reddish brown front end. SN/T 2471-201919 ????? ????, ????, ?? 7.3.5 蛹 Naked salamander, body length 2mm ~ 3mm, white, compound eyes red, body color becomes black when it is about to emerge. 7.4 Identification characteristics of Pistacia chinensis seeds 7.4.1 Female adult Body length 4mm ~ 4.5mm, black head, reddish-brown body, partial black. Feet, antennal stalk and peduncle dark yellow, whip brownish yellow, stick The knot color is lighter, the wings are yellow, the foot joints, the ends of the tibia, and the tarsus are yellow, and the ends of the tarsus, the claws, and the base of the pad are brown. The tentacles are born above the middle of the face. The tentacles are obvious and smooth, and there are flaky protrusions on both sides. Length (length is about 2.5 times the width), the length of the 5th knot is about 1.3 times the width; 3 knots of the rod, slightly longer than the sum of the last 2 knots, the boundary of the last knot is not obvious; Cable and stick sections have elongated sensors on each section. The head and chest are not deeply carved, and are white-haired; the front chest and middle chest are almost the same length, and the middle chest shield longitudinal groove is clear It is obvious that the small shield is narrow in front and wide in width, and the length and width are approximately equal, and the back of the chest is bulging; Forewing The marginal veins are longer than the bird's head-like mole veins, and the posterior marginal veins are slightly longer than the marginal veins; there are semicircular transparent sheet-like protrusions at the proximal end of the posterior margin of the hind foot basal ganglia, and the tibia has 1 Distance; posterior abdomen is shorter than chest, smooth, slightly flat, oval-shaped; abdomen is short and horizontal, with thorn-shaped protrusions on both sides; Long, slightly longer than the third quarter, only slightly plowed at the end of the abdomen, ovipositors slightly protruding. 7.4.2 Male adult Body length 3mm ~ 4mm, black, 6 tentacle cord sections, 2 rod sections, several healing. The abdominal stalk is slender, and the posterior abdomen is spindle-shaped. Feet yellow, Hind legs are slightly darker. 7.4.3 Eggs Cylindrical, milky white, with filamentous white egg stalks, the egg stalks are about the same length as the egg body, and are fixed to the inner wall of the seed. 7.4.4 Larva Old mature larvae are cricket-shaped, two pointed, wide in the middle, and the head and chest are curved toward the abdomen. The head is extremely small and ossified; the upper jaw is well developed, sickle-shaped, and tan. Present The "C" type is curved, the newly hatched larva is milky white, and the mature larva is yellow-white. 7.4.5 蛹 Liyan, body length 3.2mm ~ 4.0mm, beige, compound eyes red, female pupae brown later, male pupae black.8 result judgmentBased on the characteristics of female adults described in 7.1 and 7.2 as the main basis, supplemented by other morphological characteristics, which can be judged as larch in accordance with 7.1, 7.2, and 7.3 Seed bees, which meet 7.1, 7.2, 7.4 can be determined as Pistacia seed bees.9 Specimen preparation and preservationWith sticky shellac, stick the adult specimen on the triangular paper tip with the wings exposed to the outside, the head exposed, and the thoracic side touching the triangular paper tip; The sign indicates the collection location, host, and feathering time. Or immerse the adult specimens in absolute ethanol, preferably refrigerated. The above-mentioned specimens that have been identified should be kept permanently, and the time, place, host, and collector's information labels should be added. SN/T 2471-201919 ????? ????, ????, ??Appendix A(Informative appendix) A. 1 Biological information Larch seed bee. Harm to larch seeds, no symptoms of injury on the surface of the injured seeds. Only one larva in each seed, no risk of metastasis Harmful. Overwintering in seeds with old mature larvae, the larvae have diapause habit, which can reach 2 years. Adults emerge from the seeds and bite The small holes fly out, the feathering holes are round, and the edges are neat. Overwintering in the seed hulls and emerged every other year. A. 2 Geographical distribution Larch seed bee. Shanxi, Hebei, Shandong, Inner Mongolia, Liaoning, Jilin, Heilongjiang, Gansu; Mongolia, Japan, France, Central Asia, and Far East Coastal Area. Pistacia seed bee. Hebei, Henan, Shanxi, Shaanxi, Anwei, Shandong; Tunisia, Central Asia, Kazakhstan, Iran, Israel, Turkey. A. 3 host plants SN/T 2471-201919 ????? ????, ????, ??Appendix B(Informative appendix) Adult species search form 1． The female antennae is 10 or less, the rod is 3, the anterior wing vein is longer than the mole vein, and the male antennae is usually 5 knots. Carnivorous or herbivorous (wide The female antennae are 10 knots or less, the rod joints are 3 knots, the anterior wing veins are shorter than the mole veins, and the male antennae cord knots are usually 4 knots. Phytophagous to plant seeds 2． Phytophagous, host of plant seeds 3 Parasitic, hosted by insects 4 3． The female body is reddish brown except for the head, chest and abdomen section, and the back section of the first section of the abdomen. It is more than double, the rod section is approximately the same length as the last 2 cable sections, the male body is black, and the cable sections are markedly stalk-like; The female body is black except for the abdomen, which is slightly reddish-brown. The length of the first antennae of the antennae is only 1.5 times the width, and the length of the rods is longer than that of the last two. Belly length The length of the head and thorax combined, the length of the 7th abdominal segment is significantly larger than the width, and together with the prominent ovipositor form a plow-shaped upturn. Body length 4． The third and fourth abdomen are of equal length. The antennae are born above the middle of the face and are located on the line connecting the middle of the compound eyes. The anterior wing veins are longer than the mole veins. The 4th section of the abdomen is longer than the 3rd section. The abdomen is significantly flattened. The wing veins are longer than the mole veins, and the dorsal view of the abdomen is square, parasitic to the slime cocoon 5． The body is all black, the female anterior wing mole vein is shorter than or equal to the marginal vein, the abdomen is equal to or shorter than the length of the head and chest combined, and the fourth quarter of the abdomen is longer than the third The female anterior wing mole veins are longer than the marginal veins, and the female chest is significantly raised, and the back of the thoracoabdominal and first ventral segments are perpendicular to the body axis; the abdomen is oval, The dorsal view of the ventral stalk is twice as long as it is wide. Body length 2.5mm ～ 2.9mm, parasitic on the seeds of Chinese locust SN/T 2471-201919 ????? ????, ????, ??Appendix C(Informative appendix) Feature map Figure C. 1. Larva seed adult bee, side view C. 2 Larva seed bee, dorsal view Figure C. B. Back view of larch seed bee C. 4 side view of small bee on larch seed SN/T 2471-201919 ????? ????, ????, ?? Figure C. B. side view of a small bee seed of Pistacia chinensis C. 6 Dorsal view of Pistacia chinensis seed bees Figure C. B. View of the back of a Pistacia chinensis seed bee C. 8 Bumblebee Seed Characteristic Map SN/T 2471-201919 ????? ????, ????, ?? ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2471-2019_English be delivered?Answer: Upon your order, we will start to translate SN/T 2471-2019_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 2471-2019_English with my colleagues?Answer: Yes. 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