SN/T 2215-2008 English PDF

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SN/T 2215-2008: Determination of phenothiazines residues in animal original food for for import and export. Enzyme-linked immunosorbent assay
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 2215-2008	449	Add to Cart	3 days	Determination of phenothiazines residues in animal original food for for import and export. Enzyme-linked immunosorbent assay	Valid

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Basic data

Standard ID: SN/T 2215-2008 (SN/T2215-2008)
Description (Translated English): Determination of phenothiazines residues in animal original food for for import and export. Enzyme-linked immunosorbent assay
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Word Count Estimation: 17,134
Date of Issue: 2008-11-18
Date of Implementation: 2009-06-01
Quoted Standard: GB/T 6682
Regulation (derived from): National Quality Inspection [2008] No. 577
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the products of animal origin in the phenothiazine class of antibiotics residues in enzyme-linked immunoassay. This standard applies to meat, offal, fish and milk chlorpromazine, acepromazine, propionyl oxaprozin, oxaprozin and trifluoroacetic oxaprozin Determination of total residues.

SN/T 2215-2008: Determination of phenothiazines residues in animal original food for for import and export. Enzyme-linked immunosorbent assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of phenothiazines residues in animal original food for for import and export.Enzyme-linked immunosorbent assay Exit inspection and quarantine industry standard book People's Republic of China Import and export of animal-derived foods phenothiazines Detection of drug residues in ELISA Posted 2008-11-18 2009-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine. The main drafters. Zhang Xiaofeng, Shi Weiliang, Wangyun Quan, Zhu Zhenjiang, Fang Ying. This standard is the first release of the entry-exit inspection and quarantine industry standards. Import and export of animal-derived foods phenothiazines Detection of drug residues in ELISA

1 Scope

This standard specifies the method of enzyme-linked immunosorbent assay phenothiazines antibiotic residues in animal products. This standard applies to meat, offal, fish and milk chlorpromazine, acepromazine, propionyl oxaprozin, oxaprozin and total residual trifluoroacetic oxaprozin Determination.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Preparation and preservation of samples 3 3.1 Sample Preparation Tissue samples. all the samples taken from a representative sample of about 500g, with the organization stamp mill crushed sufficiently uniform, were divided into two, respectively Into a container, sealed, marked and labeled. Milk and milk powder. take the original packaging from a representative sample of about 500g, mix well, are divided into two, respectively, into the vessel, dense Sealed, marked and labeled. 3.2 Sample Storage The samples were stored at -18 ℃ ~ -20 ℃ condition.

4 Method summary

The standard samples were extracted with acetonitrile and basic residues chlorpromazine, acepromazine, propionyl oxaprozin, oxaprozin, and trifluoroacetic oxaprozin. Microplate package There chlorpromazine antibody was added chlorpromazine standard or sample extract and the enzyme was after chlorpromazine or free extract residues and enzyme-labeled Chlorpromazine and antibody binding competitive. Chlorpromazine and remove unbound enzyme-labeled purified by washing, followed by addition of chromogenic substrate, using a microplate reader Absorbance was measured from the absorbance worth of content in the sample residue.

5 Reagents and materials

Unless otherwise indicated, all reagents were of analytical grade water as a water GB/T 6682 regulations. 5.1 phenothiazines detection kit (see Appendix A). 5.2 acetonitrile. 5.3 40% sodium hydroxide solution. Accurately weigh 40.0g of sodium hydroxide dissolved in water and dilute to 100mL. 5.4 Standard. chlorpromazine, purity ≥98%; acepromazine, purity ≥98%; propionyl oxaprozin, purity ≥98%; oxaprozin, purity ≥98%; three Fluorine oxaprozin, purity ≥98%. 5.5 standard solution 5.5.1 Standard stock solution. Weigh 5mg (accurate to 0.1mg) chlorpromazine, acepromazine, propionyl oxaprozin, oxaprozin, trifluoropropyne Triazine, dissolved in methanol and set the volume to 10mL, to give the concentration of 500mg/L of standard stock solution. The stock solution can be -20 ℃ Protected from light and stored at 12 months. 5.5.2 intermediate mixed standard solution. Pipette a variety of standard stock solution 0.2mL (oxaprozin as 0.1mL) in a brown flask given by ......

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