SN/T 1961.12-2013 English PDFUS$209.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1961.12-2013: Detection of allergen components in food forexport. Part 12: Real time PCR method for detecting sesame components Status: Valid
Basic dataStandard ID: SN/T 1961.12-2013 (SN/T1961.12-2013)Description (Translated English): Detection of allergen components in food forexport. Part 12: Real time PCR method for detecting sesame components Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X04 Classification of International Standard: 67.050 Word Count Estimation: 8,891 Quoted Standard: GB/T 6682; GB/T 27403 Regulation (derived from): AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the food allergen ingredients sesame real-time PCR detection method. This standard applies to food and raw materials in the qualitative detection of raw sesame allergy component. The standard multi-minimum detection limit (LOD) of SN/T 1961.12-2013: Detection of allergen components in food forexport. Part 12: Real time PCR method for detecting sesame components---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Detection of allergen components in food forexport.Part 12. Real time PCR method for detecting sesame components People's Republic of China Entry-Exit Inspection and Quarantine Standards Export food allergen component testing - Part 12. Real-time PCR method for detection of sesame ingredients Part 12. RealtimePCRmethodfordetectingsesamecomponents Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordSN/T 1961 "export of food allergens detection" is divided into 19 parts. --- Part 1. enzyme-linked immunosorbent assay peanut ingredients; --- Part 2. real-time PCR was used to detect peanut ingredients; --- Part 3. enzyme-linked immunosorbent assay buckwheat protein component; --- Part 4. real-time PCR method for detection of cashew component; --- Part 5. real-time PCR method to detect pistachios ingredients; --- Part 6. real-time PCR method to detect walnut ingredients; --- Part 7. real-time PCR method to detect carrot ingredient; --- Part 8. real-time PCR method for detecting hazelnut ingredients; --- Part 9. real-time PCR method for detection of almond ingredients; --- Part 10. real-time PCR method to detect shrimp/crab ingredients; --- Part 11. real-time PCR method to detect gluten ingredients; --- Part 12. real-time PCR method for detection of sesame ingredients; --- Part 13. real-time PCR method to detect wheat component; --- Part 14. real-time PCR method to detect fish ingredients; --- Part 15. real-time PCR method for detection of celery ingredients; --- Part 16. real-time PCR method for detection of mustard ingredients; --- Part 17. real-time PCR method for detection of lupine ingredient; --- Part 18. real-time PCR method for detecting buckwheat ingredients; --- Part 19. real-time PCR method to detect soy ingredients. This section SN/T 1961 Part 12. This section drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section drafted by. China Inspection and Quarantine Science Research Institute, People's Republic of China Liaoning Exit Inspection and Quarantine, Shenzhen University. The main drafters of this section. Chen Ying, Jiang Dan, Juan Cao occasion, Ma Lidan, Moon Zheng, Liu Zhigang, Kyrgyzstan and the United States Kun, Wu order oak. Export food allergen component testing - Part 12. Real-time PCR method for detection of sesame ingredients1 ScopeSN/T 1961 This section provides the real-time PCR and fluorescence detection methods in food allergen sesame ingredients. This section allergen sesame ingredients for food and raw materials in the qualitative detection. This part of the minimum detection limit (LOD) of 0.01% (mass fraction).2 Normative referencesThe following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 27403 laboratory quality control of food molecular biology standardized testing3 Terms and DefinitionsThe following terms and definitions apply to this document. 3.1 Allergen alergen Also known as allergens or allergens, can cause allergic reactions refers to an antigen. 3.2 Sesame sesame Alias. flax, white linen, flax genus Branch (Pedaliaceae) flax genus (SesamumLinn). 3.3 Real-time fluorescence PCR realtimePCR Join fluorophore in the PCR reaction system, the use of real-time monitoring of the accumulation of the fluorescent signal of the PCR amplification process. 3.4 Ct values cyclethreshold When the number of cycles of each reaction tube fluorescent signal reaches the set value of the field experienced.4 Method summaryThe samples were milled to extract DNA, DNA as a template, use sesame housekeeping gene 2SalbuminmRNA design specific subject Test primers and probes for real-time fluorescence PCR amplification, according to the Ct value, the existence of allergen sesame ingredients Analyzing samples.5 Reagents and materialsUnless otherwise specified, reagents were analytical grade or biochemical reagents. Test water should comply with GB/T 6682 in a water specifications. All test ...... |
