SN/T 1575-2005 English PDF

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SN/T 1575-2005: Protocol of enzyme-linked immunosobent assay for chicken inclusive body hepatitis
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1575-2005	199	Add to Cart	3 days	Protocol of enzyme-linked immunosobent assay for chicken inclusive body hepatitis	Valid

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Basic data

Standard ID: SN/T 1575-2005 (SN/T1575-2005)
Description (Translated English): Protocol of enzyme-linked immunosobent assay for chicken inclusive body hepatitis
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 6,636
Date of Issue: 2005-05-20
Date of Implementation: 2005-12-01
Regulation (derived from): 2005 Bulletin No. 8 industry standard
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the chicken inclusion body hepatitis ELISA method. This standard applies to chicken inclusion body hepatitis virus antibodies.

SN/T 1575-2005: Protocol of enzyme-linked immunosobent assay for chicken inclusive body hepatitis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of enzyme-linked immunosobent assay for chicken inclusive body hepatitis People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Chicken inclusion body hepatitis ELISA Test procedure Released on.2005-05-20 Implementation of.2005-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Inner Mongolia Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Liu Middle School, Zhao Linli, Liu Hong. This standard is the first industry standard for entry-exit inspection and quarantine. Chicken inclusion body hepatitis ELISA Test procedure

1 Scope

This standard specifies the test method for chicken inclusion body hepatitis ELISA. This standard applies to the detection of chicken inclusion body hepatitis virus antibodies.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods (neq ISO 3696.1987)

3 instruments and reagents

3.1 Instrument 3.1.1 Microplate reader. 490nm wavelength filter. 3.1.2 96-well ELISA plate. 3.1.3 Micro-sampler. 50μL, 100μL. 3.1.4 Micro Oscillator. 3.1.5 incubator. 37 ° C. 3.1.6 Wet box or parafilm. 3.1.7 Syringe. 2mL with a 6 gauge needle. 3.1.8 Microcentrifuge tube. 1.5 mL. 3.1.9 Benchtop microcentrifuge. 3.2 Reagents The chemical reagents used in this standard are of analytical grade unless otherwise specified. The experimental water needs to meet the secondary water in GB/T 6682 Claim. 3.2.1 Coating solution. carbonate buffer, 0.1 mol/L, pH 9.6. See Appendix A for the preparation method. Chapter 1. 3.2.2 Washing solution. phosphate buffer (PBS-T) containing 0.05% (by volume) Tween-20, 0.01 mol/L, pH 7.4. Match See method A for the method. Chapter 2. 3.2.3 Diluent. PBS-T containing 2.5% (mass concentration) bovine serum albumin. Prepared on the day of use. 3.2.4 Substrate solution. citrate-phosphate buffer containing 0.04% (mass concentration) o-phenylenediamine and 0.1% (by volume) hydrogen peroxide Liquid, pH 5.0. See Appendix A for the preparation method. Chapter 3, the current use. 3.2.5 Stop solution. 2mol/L sulfuric acid solution. 3.2.6 Standard antigen. Chicken inclusion body hepatitis virus ELISA antigen. 3.2.7 Standard serum. positive serum, negative serum. 3.2.8 Enzyme-labeled goat anti-chicken or rabbit anti-chicken antibody.

4 blood collection

The 75% alcohol cotton ball was used to test the part of the wing vein of the wing of the chicken (the deep space between the biceps and triceps). ......

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