SN/T 1574-2005 English PDF

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SN/T 1574-2005: Protocol of enzyme-linked immunosobent assay for trichinellosis of swine
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1574-2005	199	Add to Cart	3 days	Protocol of enzyme-linked immunosobent assay for trichinellosis of swine	Valid

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Basic data

Standard ID: SN/T 1574-2005 (SN/T1574-2005)
Description (Translated English): Protocol of enzyme-linked immunosobent assay for trichinellosis of swine
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 6,631
Date of Issue: 2005-05-20
Date of Implementation: 2005-12-01
Quoted Standard: GB/T 6682
Regulation (derived from): 2005 Bulletin No. 8 industry standard
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the anti-Trichinella antibodies in pig serum enzyme-linked immunosorbent assay (ELISA) detection method. This standard applies to pig serum anti-Trichinella antibodies.

SN/T 1574-2005: Protocol of enzyme-linked immunosobent assay for trichinellosis of swine

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of enzyme-linked immunosobent assay for trichinellosis of swine Book of the People's Republic of China Entry and Exit Inspection and Quarantine Enzyme-linked immunosorbent assay protocol for porcine trichinosis Released on.2005-05-20 Implementation of.2005-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shanxi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Lian Huifeng, Li Weihua, Gong Hongxia, Fu Ying, Lian Geng. This standard is the first industry standard for entry-exit inspection and quarantine. Enzyme-linked immunosorbent assay protocol for porcine trichinosis

1 Scope

This standard specifies the method for detection of anti-Trichinella antibody by enzyme-linked immunosorbent assay (ELISA) in pig serum. This standard applies to the detection of anti-Trichinella antibodies in pig serum.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods (epvISO 3696.1987)

3 Materials and reagents

3.1 Swine Trichinosis ELISA Diagnostic Kit Store at 2 ° C ~ 8 ° C. The items that should be included in the kit are as follows. --- coated a good 96-well ELISA plate; --- Anti-Trichinella positive control serum; --- Anti-Trichinella negative control serum; ---100 times the concentration of the enzyme-labeled conjugate; --- substrate solution; ---developer (ABTS); ---terminator; --- serum and enzyme-labeled conjugates; ---10 times the concentration of the washing solution. 3.2 serum to be tested Blood was collected according to the conventional method and serum was separated and placed in a refrigerator at 4 ° C for use. If long-term storage is required, it should be set at -20 ° C.

4 instruments

4.1 Microplate reader (wavelength 405nm). 4.2 Constant temperature oscillator (37 ° C). 4.3 Autoclave. 4.4 8- or 12-channel shampoo washer. 4.5 8-channel or 12-channel pipette (50μL ~ 300μL). 4.6 Single-channel pipette (0.5μL ~ 10μL; 40μL ~.200μL;.200μL ~ 1000μL).

5 test preparation

5.1 Temperature recovery All reagents (except the enzyme-labeled conjugate) were returned to room temperature before use. 5.2 Washing liquid Take a concentrated washing solution provided in the kit and add it to nine parts of distilled water and mix for later use. Diluted distilled water should meet ......

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