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SN/T 1573-2013 English PDF

SN/T 1573: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1573-2013359 Add to Cart 3 days Determination of neurotoxic shellfish poisons in shellfish for export. Mouse biology method Obsolete
SN/T 1573-2005679 Add to Cart 5 days Neurological shellfish toxins in shellfish testing methods mouse bioassay Obsolete

Similar standards

SC/T 4030   GB 5009.261   SN/T 1151.5   SN/T 1673   SN/T 2188   

Basic data

Standard ID: SN/T 1573-2013 (SN/T1573-2013)
Description (Translated English): Determination of neurotoxic shellfish poisons in shellfish for export. Mouse biology method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B51
Classification of International Standard: 67.120.30
Word Count Estimation: 9,951
Older Standard (superseded by this standard): SN/T 1573-2005
Quoted Standard: GB/T 6682; GB 14925
Regulation (derived from): National quality recognition (2013) 569
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the method for the determination of mouse neural biological shellfish and their products shellfish toxins. This standard applies to shellfish and shellfish products nerve toxin detection.

SN/T 1573-2013: Determination of neurotoxic shellfish poisons in shellfish for export. Mouse biology method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of neurotoxic shellfish poisons in shellfish for export.Mouse biology method People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1573-2005 Export shellfish toxins in shellfish nerve detection method Mouse bioassay Issued on. 2013-11-06 2014-06-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. Instead of the standard SN/T 1573-2005 "shellfish toxins in shellfish nervous mouse bioassay test method." This standard compared with SN/T 1573-2005, in addition to editorial changes, the major technical changes are as follows. --- Increase normative references, reference standard APHA, 1985; --- Modify the terms and definitions are defined in part on mouse units; --- Modify part sample storage and preparation; --- Modify the part measurement method; --- Modify the part of the results expressed; --- Increasing the health and safety content. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine. The main drafters of this standard. Zhao Xin, Wangqiu Yan, Xu Yang, Cheng Chiu, Qu Fei, Xu Jing, Cao Juan occasion. This standard replaces the standards previously issued as follows. --- SN/T 1573-2005. Export shellfish toxins in shellfish nerve detection method Mouse bioassay

1 Scope

This standard specifies the method for determination of mouse bioassay shellfish and their products in neuropathic shellfish toxins. This standard applies to shellfish and shellfish products nerve toxin detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 14925 Laboratory Animal Facilities and Environment

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Neural shellfish toxins Neurotoxicshelfishpoisons; NSP The chemical structure of short naked dinoflagellate toxin (brevetoxin) represented after ingestion can produce neurological and gastrointestinal symptoms in the presence of shellfish meat Marine life toxic substances in general. 3.2 Rat units mouseunit; MU After weighing 20g mice by intraperitoneal injection of 1mL shellfish extract within 930min minimum required amount of toxins death A mouse units 3.3 Median mediandata The value of the variable according to the size of the order of, in the middle position is that the value of the median.

4 Method summary

NSP extracted with ether shellfish toxins, evaporated to dryness under reduced pressure, then 1% Tween -60 saline as a dispersion medium to prepare NSP- 1% Tween -60 saline suspension, the suspension was injected into the abdominal cavity, the mice were observed for survival, calculate its virulence.

5 Reagents and materials

Unless otherwise specified, reagents were analytical grade or biochemical reagents. Test water should comply with GB/T 6682 in a water specifications. 5.1 Concentrated hydrochloric acid 5.2 anhydrous ether. 5.31% Tween -60 NS. Weigh 1.0g Tween -60, dissolved in physiological saline (0.85% NaCl), and the volume to 100.0mL.
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