Home Cart Quotation About-Us
www.ChineseStandard.net
SEARCH

SN/T 1444-2004 English PDF

US$199.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 1444-2004: Indirect immunofluorescent antibody technique for epidemic hemorrhagic fever of rats
Status: Valid
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1444-2004199 Add to Cart 3 days Indirect immunofluorescent antibody technique for epidemic hemorrhagic fever of rats Valid

Similar standards

GB/T 20365   SN/T 1353   NY/T 909   SN/T 1447   SN/T 1449   SN/T 1447   

Basic data

Standard ID: SN/T 1444-2004 (SN/T1444-2004)
Description (Translated English): Indirect immunofluorescent antibody technique for epidemic hemorrhagic fever of rats
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 6,675
Date of Issue: 2004-06-01
Date of Implementation: 2004-12-01
Summary: This standard specifies the indirect immunofluorescence assay in rats epidemic hemorrhagic fever antibodies. This standard applies to rats of epidemic hemorrhagic fever quarantine, epidemiological investigation and immune monitoring.

SN/T 1444-2004: Indirect immunofluorescent antibody technique for epidemic hemorrhagic fever of rats


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Indirect immunofluorescent antibody technique for epidemic hemorrhagic fever of rats Book of the People's Republic of China Entry and Exit Inspection and Quarantine Indirect immunofluorescence test of epidemic hemorrhagic fever in rats Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Hu Yongqiang, Li Shuqing, Wang Qiaoquan, Xu Hong. This standard is the first industry standard for entry-exit inspection and quarantine. Indirect immunofluorescence test of epidemic hemorrhagic fever in rats

1 Scope

This standard specifies the method for detecting antibodies to epidemic hemorrhagic fever in rats by indirect immunofluorescence assay. This standard applies to quarantine, epidemiological investigation and immune monitoring of epidemic hemorrhagic fever in rats.

2 materials

2.1 Reagents 2.1.1 Disodium hydrogen phosphate (Na2HPO4·12H2O). 2.1.2 Potassium dihydrogen phosphate (KH2PO4). 2.1.3 Sodium chloride (NaCl). 2.1.4 Evans Blue. 2.1.5 Glycerin. 2.1.6 Preparation of 0.01mol/L pH7.4 phosphate buffer, 0.01%-0.02% Evans blue solution and 90% glycerol buffered saline Record A. Note; all chemical reagents are of analytical grade. 2.2 Instrumentation 2.2.1 Single channel adjustable pipette and dosing head. 2.2.2 Dyeing tank. 2.2.3 Humidors. 2.2.4 coverslips. 2.2.5 Thermostat. 2.2.6 Low temperature refrigerator. 2.2.7 Acidity meter. 2.2.8 Fluorescence microscope. 2.3 standard reagents 2.3.1 Porous epidemic hemorrhagic fever virus antigen tablets (preserved at -20 ° C). 2.3.2 Epidemic hemorrhagic fever positive serum. 2.3.3 Epidemic hemorrhagic fever negative serum. 2.3.4 Fluorescein isothiocyanate (FITC)-labeled rabbit anti-rat IgG fluorescent antibody.

3 sampling

The rats to be tested were aseptically collected from 0.5 mL to 1.0 mL. After the blood was coagulated, it was centrifuged at.2000 r/min for 10 min to separate the serum. serum After being inactivated at 56 ° C for 30 min, it was stored at -20 ° C for use.

4 method of operation

4.1 Preparing the antigen tablets The epidemic hemorrhagic fever virus cell antigen tablets were taken out from the low temperature refrigerator and dried by cold air.
......
Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 1444-2004_English be delivered?

Answer: Upon your order, we will start to translate SN/T 1444-2004_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 1444-2004_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 1444-2004_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.