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SN/T 1439-2013 English PDF

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SN/T 1439-2013: Molecular detection methods of Ebola virus at frontier ports
Status: Valid

SN/T 1439: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1439-2013319 Add to Cart 3 days Molecular detection methods of Ebola virus at frontier ports Valid
SN/T 1439-2004639 Add to Cart 5 days The frontier port Ebola hemorrhagic fever inspection procedures Obsolete

Similar standards

GBZ 57   GB/T 31989   SN/T 5800.2   SN/T 5800.4   SN/T 5800.1   

Basic data

Standard ID: SN/T 1439-2013 (SN/T1439-2013)
Description (Translated English): Molecular detection methods of Ebola virus at frontier ports
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C62
Classification of International Standard: 13.020
Word Count Estimation: 12,191
Older Standard (superseded by this standard): SN/T 1439-2004
Quoted Standard: GB 19489
Regulation (derived from): AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the border crossings suspected cases of Ebola haemorrhagic fever in the serum samples of biological safety requirements Ebola virus detection, specimen collection, transport and storage, handling specimens, and Zaire Ebola virus �յ�

SN/T 1439-2013: Molecular detection methods of Ebola virus at frontier ports

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Molecular detection methods of Ebola virus at frontier ports People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1439-2004 Ebola virus molecular frontier ports Biological Detection Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1439-2004 "frontier port inspection of Ebola hemorrhagic fever virus." This standard compared with SN/T 1439-2004, the main technical changes are as follows. --- Modify the standard English name; --- Original standard detection methods provide, such as virus isolation, electron microscopy, indirect immunofluorescence assay, Western blot test All related live virus, must be carried out in a BSL-4 laboratory, basically it does not have the maneuverability, therefore, the standard combination Bio-safety requirements, the original standard, comprehensive method has been modified. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Huang Ji City, Li Yan, Shi Yong Xia, Cheng Kui, Li Xiaobo, Hong Ye Xing Lu Qin, phase Dapeng, Gobo rotation, Zhong Yuqing, Mo Qiuhua, Shi Yongmei. This standard replaces the standards previously issued as follows. --- SN/T 1439-2004. Ebola virus molecular frontier ports Biological Detection

1 Scope

This standard specifies the border crossings suspected cases of Ebola haemorrhagic fever in serum samples required for biosafety Ebola virus detection specimen Collection, transport and storage, handling specimens 苏丹埃博拉 Zaire Ebola virus and conventional RT-PCR and real-time fluorescence detection RT-PCR, and detection of Ebola gene chip method. This standard applies to the border port of entry and exit personnel suspected Ebola haemorrhagic fever cases detected in clinical specimens biological molecules.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Ebola hemorrhagic fever Ebolahemorrhagicfever; EHF By the Ebola virus (Ebolavirus, EBV) causes an acute hemorrhagic diseases. People mainly through contact with infected patients or move Humoral substance, excretions, secretions and infection, clinical manifestations were fever, bleeding and multiple organ damage. Ebola hemorrhagic fever mortality High, up to 50% to 90%. The disease in the 1970s, first discovered in Africa, mainly in Africa, Uganda, Congo, Gabon, the Soviet Union Dan, Ivory Coast, Liberia, South Africa and other endemic countries. 3.2 Ebola Ebolavirus Also known as Ebola hemorrhagic fever virus, is a filamentous virus family as a single strand of negative-strand RNA virus, which is known to man since the most terrible disease One drug, the World Health Organization (WHO) as a potential biological warfare agent pathogens. It can be divided into four distinct subtypes. Zaire Ebola (EBO-Z), 苏丹埃博拉 (EBO-S), Côte d'Ivoire Ebola (EBO-C) and Ebola Reston (EBO-R). The first three subtypes may Man and primate disease.

4 Abbreviations

The following abbreviations apply to this document. RT-PCR. reverse transcription - polymerase chain reaction Real-time fluorescent RT-PCR. Real-time fluorescence reverse transcription - polymerase chain reaction When the number of cycles of each reaction tube fluorescent signal reaches the threshold set by experienced. Ct values RNA. ribonucleic acid FAM. FAM fluorescent dye, a fluorescent reporter group
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