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SN/T 1421-2004 English PDF

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US$319.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 1421-2004: The pig water herpes virus trace serum and test
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1421-2004	319	Add to Cart	3 days	The pig water herpes virus trace serum and test	Obsolete

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Basic data

Standard ID: SN/T 1421-2004 (SN/T1421-2004)
Description (Translated English): The pig water herpes virus trace serum and test
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 8,821
Date of Issue: 2004-06-01
Date of Implementation: 2004-12-01
Regulation (derived from): State Accreditation Science (2012) 57
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the trace swine vesicular disease virus serum neutralization test methods. This standard applies to vesicular disease virus neutralizing antibodies.

SN/T 1421-2004: The pig water herpes virus trace serum and test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
The pig water herpes virus trace serum and test People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Micro-serum neutralization test of swine vesicular disease virus Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix, and Appendix B is an informative appendix. This standard is proposed and managed by the National Certification and Accreditation Supervision Committee. This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Yang Su, Pan Wenbo, Liao Xiuyun, Feng Jiawang. This standard is the first industry standard for entry-exit inspection and quarantine. Micro-serum neutralization test of swine vesicular disease virus

1 Scope

This standard specifies the micro-serum neutralization test method for swine vesicular disease virus. This standard applies to the detection of neutralizing antibodies to swine vesicular disease virus.

2 Abbreviations

The following abbreviations apply to this standard 2.1 CPE. cytopathic effect. 2.2 TCID50. Half of the amount of cell infection. 2.3 SVD. swine vesicular disease.

3 Principle

The specific serum neutralizing antibody binds to the virus, which can make the virus lose its ability to infect sensitive cells, thus preventing the virus from spreading. Colonization. This reaction is manifested not only as a virus can only be neutralized by the corresponding immune serum, but also in neutralizing a certain amount of virus, Must have a certain titer of immune serum. The neutralization test specified in this standard is based on quantitative virus dilution serum, when serum can prevent quantification When the virus is 50% cytopathogenic, its serum dilution is its potency. Therefore, the neutralization test is a quantitative method for the determination of swine vesicular disease. Method of serum antibody.

4 reagents and materials

4.1 See Appendix A for the preparation of reagents used in this standard. 4.2 Unless otherwise specified, the reagents of this standard refer to analytical reagents. 4.3 Reagents and glassware used in this standard are autoclaved except for special regulations. 4.4 Virus. Porcine vesicular disease virus purified by plaque method and serologically identified as a standard virus. 4.5 Standard serum. standard negative serum using pig serum without SVD virus antibody, standard strong positive and weak positive serum using SVD disease Pig serum recovered after the onset of poison infection. 4.6 Cell line. IB-Rs-2 passage cell line. 4.7 Cell culture medium. Cell culture medium containing 10% fetal bovine serum (completed at 56 ° C for 30 min) and antibiotics. 4.8 Cell Maintenance Solution. A cell maintenance solution containing 2% fetal bovine serum (50 ° C, 30 min extinguished) and antibiotics. 4.9 Cell dispersion. 4.10 Fixative solution. 4.11 Staining solution.

5 instruments and equipment

5.1 Carbon dioxide incubator, micro-oscillator, inverted microscope, refrigerator, ultrasonic cell disruptor. 5.2 100mL cell culture flask, 96-well micro-cell culture plate, tip, multi-channel adjustable pipette, single-channel adjustable pipette. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 1421-2004_English be delivered?

Answer: Upon your order, we will start to translate SN/T 1421-2004_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 1421-2004_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 1421-2004_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.