SN/T 1418-2004 English PDF

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SN/T 1418-2004: Serum neutralization test for duck virus hepatitis type Ⅰ virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1418-2004	259	Add to Cart	3 days	Serum neutralization test for duck virus hepatitis type Ⅰ virus	Valid

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Basic data

Standard ID: SN/T 1418-2004 (SN/T1418-2004)
Description (Translated English): Serum neutralization test for duck virus hepatitis type �� virus
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 8,877
Date of Issue: 2004-06-01
Date of Implementation: 2004-12-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the duck hepatitis virus type I serum neutralization test method of operation. This standard applies to detect serum antibodies duck hepatitis virus type I.

SN/T 1418-2004: Serum neutralization test for duck virus hepatitis type Ⅰ virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Serum neutralization test for duck virus hepatitis type I virus Book of the People's Republic of China Entry and Exit Inspection and Quarantine Serum neutralization test of duck viral hepatitis type I virus Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix, and Appendix B is an informative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Chen Yu, Huang Xinmin, Xu Haini, Yang Su, Pan Wenbo, Liao Xiuyun. This standard is the first industry standard for entry-exit inspection and quarantine. Serum neutralization test of duck viral hepatitis type I virus

1 Scope

This standard specifies the serum neutralization test method for duck viral hepatitis type I virus. This standard applies to the detection of duck viral hepatitis type I virus antibodies in serum. Mainly used for the diagnosis and pathogen identification of duck viral hepatitis Monitoring, immunization level monitoring and epidemiological investigation.

2 Abbreviations

The following abbreviations apply to this standard. 2.1 BME. Eagle's Basal Medium cell base salt medium. 2.2 CPE. cytopathic. 2.3 DEK. duck embryo kidney cells. 2.4 DEL. Duck embryo liver cells. 2.5 DHV-I. Duck viral hepatitis type I virus. 2.6 FCS. fetal bovine serum. 2.7 MEM. Eagle's MinimalEssentialMedium cell culture medium. 2.8 PFU. plaque forming unit. 2.9 TCID50. Half of the cell culture infection.

3 Principle

After the virus infects the sensitive cell monolayer, it can grow and proliferate on the cell monolayer, and can produce CPE, causing the infected cells to shrink and necrosis. And the formation of plaques, specific antibodies in the serum can inhibit the growth of the virus on the cell monolayer, the ability of serum antibodies to neutralize the virus can pass It measures the extent to which CPE is prevented from being produced. This neutralization test is a method of using a fixed amount of virus to dilute serum, in DEL or DEK Neutralization tests were performed on the cell monolayer for the determination of duck serum antibodies or the identification of pathogens.

4 instruments

4.1 High pressure steam sterilizer. 4.2 Adjustable pipettes. 4.3 Carbon dioxide constant temperature incubator. 4.4 Invert the microscope. 4.5 Sterilization filter. ......

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