SN/T 1379.3-2006 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1379.3-2006	319	Add to Cart	3 days	Protocol of immuno-fluoresent neutralization test for classic swine fever	Obsolete

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GB/T 20365   SN/T 1353   NY/T 909   SN/T 1370   SN/T 1378   SN/T 1379   

Basic data

Standard ID: SN/T 1379.3-2006 (SN/T1379.3-2006)
Description (Translated English): Protocol of immuno-fluoresent neutralization test for classic swine fever
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 8,846
Date of Issue: 2006-01-26
Date of Implementation: 2006-08-16
Quoted Standard: GB/T 6682
Regulation (derived from): ?Industry Standard Announcement 2006 No.4 (Total No.76); National-Quality-Inspection-accreditation (2010) 582
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the technical requirements of swine plague fluorescent antibody detection. This section applies to swine plague detection and antibody levels.

SN/T 1379.3-2006: Protocol of immuno-fluoresent neutralization test for classic swine fever

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of immuno-fluoresent neutralization test for classic swine fever Book of the People's Republic of China Entry and Exit Inspection and Quarantine Swine fever neutralization immunofluorescence test protocol Released on.2006-01-26 Implementation of.2006-08-16 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 1379 is divided into three parts. --- Swine fever monoclonal antibody enzyme-linked immunosorbent assay; --- Operating procedures for swine fever immunofluorescence technology; --- Swine fever neutralization immunofluorescence test protocol. This part is the third part of SN/T 1379. Appendix A of this section is a normative appendix. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Dong Zhizhen, Zhao Xiangping, Huo Lei, Cai Guorui, Jiang Hong, Wang Yuling. This section is the first industry standard for entry-exit inspection and quarantine. Swine fever neutralization immunofluorescence test protocol

1 Scope

This part of SN/T 1379 specifies the technical requirements for the detection of swine fever immunofluorescent antibodies. This section applies to the detection of swine fever virus neutralizing antibody levels.

2 Normative references

The provisions in the following documents become the provisions of this part by reference to this part of SN/T 1379. Quotations with dated , all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouragement is achieved under this section The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to this section. GB/T 6682 Analytical laboratory water specifications and test methods

3 reagents and materials

3.1 Reagents 3.1.1 Experimental distilled water is the third grade water in GB/T 6682. 3.1.2 PK-15 cells. 3.1.3 Virus. swine fever (HC) Thiverval attenuated freeze-dried strain. Provided by the designated unit, dissolved in sterile three-stage water before use Volume. 3.1.4 HC negative, positive reference serum, provided by the designated unit, inactivated at 56 ° C, 30 min before the test. 3.1.5 HC trivalent fluorescent antibody working solution, provided by the designated unit. 3.1.6 Cell dilution. see section A. Chapter 1. 3.1.7 Cell growth fluid. see section A. Chapter 2. 3.1.8 Acetone (preserved at -30 ° C). 3.1.9 Bovine serum albumin (BSA). 3.1.10 Cell Digestion. See Section A. Chapter 3. 3.2 Materials 3.2.1 Ultra-clean workbench. 3.2.2 Carbon dioxide incubator. 3.2.3 Inverted fluorescence microscope. 3.2.4 Adjustable micropipettes and corresponding dripper. 3.2.5 96-well micro-cell culture plates. 3.2.6 Incubator. 3.2.7 Microplate oscillator. 3.3 Tested serum 3.3.1 Aseptically take the blood of the animal to be tested and separate the serum. The test serum was inactivated at 56 ° C for 30 min before the experiment. 3.3.2 Serum transport and preservation. Refrigeration methods are required to transport and preserve serum. ......

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