SN/T 1379.2-2005 English PDF

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SN/T 1379.2-2005: Protocol of direct immunofluorescent assay for classical swine fever
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1379.2-2005	359	Add to Cart	3 days	Protocol of direct immunofluorescent assay for classical swine fever	Obsolete

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Basic data

Standard ID: SN/T 1379.2-2005 (SN/T1379.2-2005)
Description (Translated English): Protocol of direct immunofluorescent assay for classical swine fever
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 9,959
Date of Issue: 2005-08-18
Date of Implementation: 2006-02-01
Quoted Standard: GB/T 6682
Regulation (derived from): Industry Standards Bulletin 2005/(total No. 70) 12; national quality recognition [2010] No. 582
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies: classical swine fever immunofluorescence method of operation, This standard applies to: classical swine fever virus the detection and identification,

SN/T 1379.2-2005: Protocol of direct immunofluorescent assay for classical swine fever

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of direct immunofluorescent assay for classical swine fever Book of the People's Republic of China Entry and Exit Inspection and Quarantine Hog 瘟 immunofluorescence technology operating procedures Released on.2005-08-18 2006-02-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix, and Appendix B is an informative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Henan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhang Juzhou, Miao Li, Yang Xiangying, Jiang Zhiyi, Zhang Zihong, Li Wei. This standard is the first industry standard for entry-exit inspection and quarantine. Hog 瘟 immunofluorescence technology operating procedures

1 Scope

This standard specifies the operation method of swine fever immunofluorescence technology. This standard applies to the detection and identification of swine fever virus.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this standard. 3.1 Immunofluorescence test. 3.2 Classical piglet (commonly known as piglet). 3.3 Fluorescein isothiocyanate. 3.4 Pig kidney passage cells. 3.5 Bovine viral diarrhea virus/bovine mucosal disease virus

4 Principle

The immunofluorescence technique is to freeze the section of the diseased material or proliferate the virus by cover glass culture, and then place it on a solid phase carrier and label it with fluorescein. The antibody reacts with it to form a viral antigen-fluorescein-labeled antibody complex, and fluorescence is observed under a fluorescence microscope. This standard uses a clean slide as a carrier, and freezes the diseased material or the cell culture virus proliferation cover slip on the slide, and adds The FITC-labeled swine fever antibody was observed under a fluorescence microscope after completion of the reaction.

5 Equipment and reagents

5.1 Equipment 5.1.1 Fluorescence microscope. 5.1.2 Frozen slicer. ......

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