SN/T 1377-2004 English PDF

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SN/T 1377-2004: Horse viral arteritis virus isolated from semen test operating procedures
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1377-2004	239	Add to Cart	2 days	Horse viral arteritis virus isolated from semen test operating procedures	Obsolete

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Basic data

Standard ID: SN/T 1377-2004 (SN/T1377-2004)
Description (Translated English): Horse viral arteritis virus isolated from semen test operating procedures
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 6,657
Date of Issue: 6/1/2004
Date of Implementation: 2004-12-01
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the horse isolated from semen viral arteritis virus test methods. This standard applies to horses isolated from semen viral arteritis virus.

SN/T 1377-2004: Horse viral arteritis virus isolated from semen test operating procedures

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Horse viral arteritis virus isolated from semen test operating procedures People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Isolation of equine viral arteritis virus from semen Test procedure Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Liang Chengzhu, Zhu Laihua, Wang Shufeng, Wang Li. This standard is the first industry standard for entry-exit inspection and quarantine. Isolation of equine viral arteritis virus from semen Test procedure

1 Scope

This standard specifies the test method for the isolation of equine viral arteritis virus from semen. This standard applies to the isolation of equine viral arteritis virus from semen.

2 Abbreviations

The following abbreviations apply to this standard. 2.1 EAV. equine viral arteritis virus. 2.2 MEM. Cell culture medium containing non-essential amino acids.

3 instruments

3.1 Ultra-clean workbench or biosafety cabinet. 3.2 Carbon dioxide incubator. 3.3 Invert the microscope. 3.4 Liquid nitrogen storage tank. 3.5 Chua's filter and microporous membrane (0.22 μm). 3.6 Steam sterilizer. 3.7 Micro Cell Culture Plate (96 well). 3.8 Micro Oscillator. 3.9 Adjustable micropipettes and nozzles (200 μL). 3.10 Electronic balance (0.1mg ~.200g). 3.11 Pipette (1 mL, 2 mL, 5 mL, 10 mL).

4 reagents

4.1 Antibiotic stock solution. 4.2 7.5% sodium bicarbonate solution. 4.3 Cell Digestion (0.02% EDTA). 4.4 Standard virus strains and cell lines. -196 ° C (liquid nitrogen) frozen for use. 4.4.1 Standard virus strain. Equine Arteritis Virus (EAV) Bucyrus strain. 4.4.2 Cell line. rabbit kidney cells (RK-13). 4.5 Cell culture fluid and cell maintenance fluid. 4.6 1% cell staining solution. 4.7 7.5% carboxymethylcellulose.

5 Method of operation

5.1 Semen collection 5.1.1 Prepare a clean, sterile collection tube (eg, a fake vagina, condom). The collection tube must not contain preservatives or disinfectants. ......

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