SN/T 1376.2-2004 English PDF

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SN/T 1376.2-2004: Contagious bovine pleuropneumonia complement fixation test methods
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1376.2-2004	399	Add to Cart	3 days	Contagious bovine pleuropneumonia complement fixation test methods	Obsolete

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Basic data

Standard ID: SN/T 1376.2-2004 (SN/T1376.2-2004)
Description (Translated English): Contagious bovine pleuropneumonia complement fixation test methods
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 10,161
Date of Issue: 2004-06-01
Date of Implementation: 2004-12-01
Regulation (derived from): State Accreditation Science (2012) 57
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the contagious bovine pleural complement fixation test methods. This standard applies to infectious bovine serological diagnosis of pleurisy.

SN/T 1376.2-2004: Contagious bovine pleuropneumonia complement fixation test methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Contagious bovine pleuropneumonia complement fixation test methods Book of the People's Republic of China Entry and Exit Inspection and Quarantine Bovine infectious pleuropneumonia complement fixation test method Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A and Appendix B of this standard are normative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Sichuan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Li Yubing, Yan Yubao, Cao Zongjun, Hu Juan, Ma Yong. This standard is the first industry standard for entry-exit inspection and quarantine. Bovine infectious pleuropneumonia complement fixation test method

1 Scope

This standard specifies the method of complement binding test for bovine infectious pleuropneumonia. This standard applies to the serological diagnosis of bovine infectious pleuropneumonia.

2 reagents and materials

2.1 Materials 2.1.1 Glass test tubes. 2.1.2 96-well blood coagulation plate. 2.1.3 Adjustable sampler. 2.1.4 Test tube rack. 2.1.5 General centrifuge, 96-well plate centrifuge. 2.1.6 Water bath. 2.1.7 Ordinary refrigerator, ordinary freezer. 2.1.8 Micro-oscillator. 2.2 Reagents 2.2.1 Diluent. Sterilized Barbital Buffer (referred to as VB liquid) See Appendix A. 2.2.2 Bovine Infectious Pleuropneumonia Complement Binding Test Antigen. Reference by Bovine Infectious Pleuropneumonia by World Organisation for Animal Health (OIE) Provided by the laboratory and used according to the instructions when using. 2.2.3 Bovine Infectious Pleuropneumonia Complement Binding Test Standard Negative Serum, Standard Positive Serum. Reference by OIE Bovine Infectious Pleuropneumonia Provided by the laboratory. Diluted 1.10 times before use and inactivated in a 56 ° C water bath for 30 min. 2.2.4 Hemolysin. 2.2.5 Complement. 2.2.6 6% sheep red blood cell suspension. see Appendix B. 2.2.7 Tested serum. collected, separated, transported and stored in the usual way. 1.10 dilution before use, inactivated at 56 ° C water bath 30min.

3 preliminary test

3.1 Determination of hemolysin titer 3.1.1 Preparation of 1.20 complement 0.5 mL of complement was added to 9.5 mL of VB solution and diluted into a 1.20 complement ice bath for use. 3.1.2 Preparation of 1.100 hemolysin 0.1 mL of the hemolysin stock solution was taken, and 9.9 mL of the VB solution was added and mixed to prepare a 1.100-fold diluted hemolysin. 3.1.3 Preparation of different dilutions of hemolysin solution The hemolysin was diluted with VB solution according to Table 1 with 1.100 hemolysin and mixed. ......

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