SN/T 1358.1-2004 English PDF

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SN/T 1358.1-2004: Equine infectious anemia, complement fixation test methods
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1358.1-2004	359	Add to Cart	3 days	Equine infectious anemia, complement fixation test methods	Obsolete

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Basic data

Standard ID: SN/T 1358.1-2004 (SN/T1358.1-2004)
Description (Translated English): Equine infectious anemia, complement fixation test methods
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 9,972
Date of Issue: 2004-06-01
Date of Implementation: 2004-12-01
Regulation (derived from): CNCA notice 2006. 6. 21 (Inspection and Quarantine announced on industry standards review and cleanup conclusions notification)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the equine infectious anemia complement fixation test methods. This standard applies to equine infectious anemia antibody status.

SN/T 1358.1-2004: Equine infectious anemia, complement fixation test methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Equine infectious anemia, complement fixation test methods Book of the People's Republic of China Entry and Exit Inspection and Quarantine Horse infectious anemia complement test method Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix, and Appendix B is an informative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Wang Hao, Liu Binglan, Wu Jianping, Qu Guiwei. This standard is the first industry standard for entry-exit inspection and quarantine. Horse infectious anemia complement test method

1 Scope

This standard specifies the test method for complementation of equine infectious anemia. This standard applies to the detection of antibody levels in equine infectious anemia.

2 Instruments and equipment

2.1 Incubator. 2.2 Refrigerator. 2.3 Constant temperature water bath. 2.4 Centrifuge, centrifuge tube. 2.5 balance (sensing 0.1g). 2.6 plate. size 90mm × 15mm. 2.7 Erlenmeyer flask (50 mL to 250 mL). 2.8 Test tube and test tube holder. specifications 12×37mm, 10×100mm. 2.9 Straw. Specifications 1mL, 5mL, 10mL. 2.10.200 μL adjustable micropipette and tip.

3 materials

3.1 Reagents. Sodium chloride, calcium chloride, magnesium chloride, glucose, sodium citrate, barbital sodium, barbital, gelatin are all analytically pure. 3.2 Preparation of the solution. See Appendix A. 3.3 Antigen. The detection of two antigens of the same batch using the standard antigen, namely V antigen (inoculated antigen), C antigen (control antigen). 3.4 Standard positive serum and negative serum. provided by the pharmaceutical company that sells the antigen. 3.5 Tested serum. See Appendix B. 3.6 Complement. See Appendix B. 3.7 Hemolysin. purchased by biopharmaceutical factory, kept in the refrigerator for use (because hemolysin is added with the same amount of glycerin for preservation, it should be used when The oil was deducted, that is, the actual amount of hemolysin in 2 mL was 1 mL). 3.8 Red blood cell fluid. See Appendix B.

4 preliminary test

4.1 Determination of complement titer Fresh complement or lyophilized complement was diluted to a range of different concentrations in Table 1 for use. Table 1 Complement dilution method is in milliliters ingredient 1 2 3 4 5 6 7 8 9 10 11 complement GVB fluid ......

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