SN/T 1357-2004 English PDF

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SN/T 1357-2004: The Ibaraki disease immunity agar diffusion test methods
Status: Obsolete

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1357-2004	239	Add to Cart	2 days	The Ibaraki disease immunity agar diffusion test methods	Obsolete

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Basic data

Standard ID: SN/T 1357-2004 (SN/T1357-2004)
Description (Translated English): The Ibaraki disease immunity agar diffusion test methods
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 6,697
Date of Issue: 2004-06-01
Date of Implementation: 2004-12-01
Regulation (derived from): CNCA notice 2006. 6. 21 (Inspection and Quarantine announced on industry standards review and cleanup conclusions notification)
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the bovine immune disease Ibaraki agar diffusion test methods. This standard applies to the entry and exit quarantine cattle Ibaraki disease.

SN/T 1357-2004: The Ibaraki disease immunity agar diffusion test methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
The Ibaraki disease immunity agar diffusion test methods People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Ibaraki disease immune agar diffusion test method Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Liu Youqing, Lu Xiaozhong, Qu Fengqin, Pan Xuechang, Jiang Yong. This standard is the first industry standard for entry-exit inspection and quarantine. Ibaraki disease immune agar diffusion test method

1 Scope

This standard specifies the Niucheng disease immune agar diffusion test method. This standard applies to the quarantine of cattle and Ibaraki disease in and out of the country.

2 materials

2.1 Reagents 2.1.1 Agarose (for electrophoresis), 0.9% saline. 2.1.2 antigen, standard positive serum. 2.2 Equipment 2.2.1 Straws, graduated cylinders, flasks, 90 mm diameter plates. 2.2.2 Water platform, balance, metal puncher, adjustable micropipette and tip. 2.2.3 Template paper. Six holes around one hole in the middle, the hole diameter is 4mm; the hole spacing (edge) is 3mm. 2.3 Tested serum The blood was collected aseptically, and the serum was separated and stored at 4 ° C or frozen.

3 method of operation

3.1 Preparation of agar plates Take 1 g of agarose, 100 mL of 0.9% physiological saline, add to the Erlenmeyer flask, and fully boil and melt in a water bath, or 115 ° C for 10 min. Pressurize and cool to about 56 °C. Place the clean dry heat-sterilized plate on the water platform, add 20mL (thickness 3mm) to each plate, and cover the solidification. After that, put the plate upside down to prevent evaporation of water and store at 4 ° C. 3.2 punching The reaction well is now used, the agar gel plate is taken out from the refrigerator, placed on the template paper, and the pattern is in a fixed position, and the metal is punched immediately. Punch the hole and gently pick out or suck the agar column in the hole with a needle. Do not hurt the edges. 3.3 Loading The plate with the hole type is written with the date and number, and the sample is loaded with an adjustable micropipette. When using Figure 1, add the antigen to the "G" well, using Figure 2. At the time, the antigen was added to the central well, and the "+" well plus the standard positive serum was added to the other wells. Each hole is filled with no overflow, each plus one One sample should be replaced with one. Then place the agar plate in a wetted lid with wet gauze and let it spread at room temperature (20 ° C ~ 25 ° C). reaction. ......

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