SN/T 1247-2022 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1247-2022	RFQ	ASK	3 days	(Technical Specification for Quarantine of Porcine Reproductive and Respiratory Syndrome)	Valid
SN/T 1247-2007	679	Add to Cart	3 days	Quarantine protocol for porcine reproductive and respiratory syndrome	Obsolete
SN/T 1247.1-2003	279	Add to Cart	3 days	Protocol of indirect immunofluorescence assay for porcine reproductive and respiratory syndrome	Obsolete

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Basic data

Standard ID: SN/T 1247-2022 (SN/T1247-2022)
Description (Translated English): (Technical Specification for Quarantine of Porcine Reproductive and Respiratory Syndrome)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 65.020.30
Date of Issue: 2022-07-07
Date of Implementation: 2023-02-01
Older Standard (superseded by this standard): SN/T 1247-2007
Issuing agency(ies): General Administration of Customs

SN/T 1247-2007: Quarantine protocol for porcine reproductive and respiratory syndrome

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for porcine reproductive and respiratory syndrome Exit inspection and quarantine industry standard book People's Republic of China Instead of the SN/T 1247.1 ~ 1247.2-2003 Porcine reproductive and respiratory syndrome quarantine norms Posted 2007-08-06 2008-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

Instead of the standard SN/T 1247.1-2003 "porcine reproductive and respiratory syndrome, indirect immunofluorescence test" and SN/T 1247.2-2003 "Porcine reproductive and respiratory syndrome enzyme immune perchlorate single test." This standard Annex A, Annex B, Annex C and Annex D is informative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine of Liaoning People's Republic of China Bureau, People's Republic of China Tianjin Exit Inspection and Quarantine. The main drafters of this standard. Sun Yingjie, Zhang Boqiang, Baiquan Yang, Su Yongsheng, Jane Friends Yuanwen Ze, Zhao Xiangping, Dong Zhizhen. This standard replaces the standards previously issued as follows. --- SN/T 1247.1-2003; --- SN/T 1247.2-2003. Porcine reproductive and respiratory syndrome quarantine norms

1 Scope

This standard specifies the porcine reproductive and respiratory syndrome (PRRS) quarantine standards. Virus isolation and identification This standard applies to porcine reproductive and respiratory syndrome virus antigen in monitoring, detecting antibodies to the virus, the disease can be used Quarantine, diagnosis and epidemiological investigation.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 18090-2000 porcine reproductive and respiratory syndrome diagnosis

3 Terms and Definitions

The following terms and definitions apply to this standard. 3.1 Culture monolayers inoculated PRRS virus or treated test sample, so that 30% to 50% of the cells infected by the organization and internal fixation Source enzyme treatment, add a subject or a positive serum antibody treatment, plus anti-enzyme antibody, through a color reaction for detecting the presence of an antigen or antibody. in PRRS was first used diagnostically to detect the antibodies. 3.2 Into slices or the smear samples after a fixed, plus antiserum treatment, together with a fluorescent labeled anti-antibody staining, rinsed, buffer solution of glycerol After sealing with a cover slip load, detected by fluorescence microscopy. Detection antibody can be used for early diagnosis.

4 Disease Overview

Porcine reproductive and respiratory syndrome (porcinereproductiveandrespiratorysyndrome, PRRS), also known as blue-ear disease (Blue-earedDisease), is composed of porcine reproductive and respiratory virus (porcinereproductiveandrespiratorysyndrome syndrome virus, PRRSV) causes reproductive failure in sows and piglets and finishing pigs characterized by breathing difficulties contagious disease. Reproductive disorders Characterized in miscarriage, stillbirth and low earners yield, low earners since the respiratory disease and secondary infections often die soon; respiratory tract characterized piglets and breeding Pigs breathing difficulties, older pigs may appear mild respiratory symptoms, sometimes leaving the complex disease due to secondary infection. Pathogen, popular Epidemiology, diagnosis, and quarantine and diagnosis in Appendix A.

5 quarantine methods

5.1 Virus isolation and identification (see GB/T 18090-2000) 5.1.1 Equipment, materials and reagents 5.1.1.1 Equipment. 96- or 48-well cell culture plate, micropipette, constant temperature water bath, carbon dioxide incubator, ordinary refrigerator and low temperature Refrigerators, centrifuges and centrifuge tubes, tissue grinder, pore size 0.2μm microporous membrane, ordinary optical microscope. 5.1.1.2 Reagents. RPMI1640 culture medium, calf serum, penicillin (104IU/mL) and streptomycin (104μg/mL) solution, 7.5% Sodium bicarbonate solution and the like. ......

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