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SN/T 1221-2016 English PDF

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SN/T 1221-2016: Quarantine protocol for chicken infectious bronchitis
Status: Valid

SN/T 1221: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1221-2016369 Add to Cart 3 days Quarantine protocol for chicken infectious bronchitis Valid
SN/T 1221-2015RFQ ASK 3 days (Avian infectious bronchitis and Quarantine Technical Specifications) Obsolete
SN/T 1221-2003199 Add to Cart 2 days Detection of antibodies against avian infections bronchitis. Protocol of agar gel immunodiffusion test Obsolete

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Basic data

Standard ID: SN/T 1221-2016 (SN/T1221-2016)
Description (Translated English): Quarantine protocol for chicken infectious bronchitis
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 16,117
Date of Issue: 2016-03-09
Date of Implementation: 2016-10-01
Older Standard (superseded by this standard): SN/T 1221-2003
Regulation (derived from): National Quality Inspection [No. 2016] No. 131; Industry Standard Record Announcement No. 2016 (No. 201)
Issuing agency(ies): General Administration of Customs

SN/T 1221-2003: Detection of antibodies against avian infections bronchitis. Protocol of agar gel immunodiffusion test


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of antibodies against avian infections bronchitis.Protocol of agar gel immunodiffusion test Book of the People's Republic of China Entry and Exit Inspection and Quarantine Chicken infectious bronchitis antibody detection method Agar immunodiffusion test Released on.2003-05-28 Implementation of.2003-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Liang Yade, Liu Zhiguang, Yang Baohua. This standard is the industry standard for inspection and quarantine issued for the first time. Chicken infectious bronchitis antibody detection method Agar immunodiffusion test

1 Scope

This standard specifies methods for the detection of antibodies to infectious bronchitis in chickens using the agar immunodiffusion test. This standard is applicable to the detection of antibodies to infectious bronchitis in chickens and the diagnosis, quarantine and epidemic monitoring of the disease.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this standard. 3.1 OIE officeinternationaldesepizooties International Organization of Animal Health 3.2 IB avianinfectiousbronchitis infectious bronchitis 3.3 IBV avianinfectiousbronchitisvirus avian infectious bronchitis virus

4 Test methods

4.1 Principle Agar is a polysaccharide containing a sulfate group. It dissolves in water at high temperature, solidifies upon cooling, forms a gel, and the agar gel is porous. The pore size depends on the concentration of the agar gel. The 1% agar gel has a pore size of about 85 nm. This pore size range allows for various antigens. The antibody molecule diffuses freely in the agar gel and forms a concentration gradient from near to far. When the two meet at a proper ratio, the precipitation occurs. should. The precipitate formed by the antigen-antibody reaction is no longer diffused in the gel due to its large particle size, but forms a precipitated band. According to the presence or absence of precipitation A determination is made as to whether a corresponding antigen-antibody immune response exists to determine whether a corresponding antibody or antigen is present in the sample. 4.2 Instrument and test preparation 4.2.1 Standard antigen and standard positive serum Standard antigen and IBV standard positive sera are provided by the OIE Reference Laboratory or designated unit. 4.2.2 Preparation of agarose gel plate Dissolve 0.9% to 1% agarose with 1.5mol/L sodium chloride (NaCl) solution, dissolve in water or dissolve in high pressure, and cool to 55 °C. 15 mL of the left and right were poured into a 9 cm-diameter plate to prepare an agarose gel plate. The thickness of the agarose gel was about 2 mm, and it was set aside after cooling. 4.2.3 Instruments --- Balance (0.001g); ---Constant temperature water bath;
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