SN/T 1207-2011 English PDF

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SN/T 1207-2011: Quarantine protocol for swine dysentery
Status: Valid

SN/T 1207: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1207-2011	339	Add to Cart	3 days	Quarantine protocol for swine dysentery	Valid
SN/T 1207-2003	279	Add to Cart	3 days	Protocol of isolation and culture of brachyspira hyodysenteriae for swine dysentery	Obsolete

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Basic data

Standard ID: SN/T 1207-2011 (SN/T1207-2011)
Description (Translated English): Quarantine protocol for swine dysentery
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 13,164
Date of Issue: 2011-05-31
Date of Implementation: 2011-12-01
Older Standard (superseded by this standard): SN/T 1207-2003
Quoted Standard: GB/T 6682; GB 19489; GB/T 19495.3
Regulation (derived from): National-Quality-Inspection-accreditation (2011) 291
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the clinical diagnosis of swine dysentery disease, tissue biopsy and microscopic examination of the body B. hyodysenteriae Isolation and identification methods. This standard applies to quarantine the diagnosis of swine dysentery disease.

SN/T 1207-2011: Quarantine protocol for swine dysentery

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for swine dysentery People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1207-2003 Swine dysentery quarantine Technical Specifications Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1207-2003 "swine dysentery Brachyspira Isolation procedures." This standard compared with SN/T 1207-2003, the main technical changes are as follows. --- Increase the clinical diagnosis of swine dysentery and diseases related technical overview of the content; --- Increased prevalence of pig intestine tissue sections microscopy method of operation content; --- Amend and improve the body B. hyodysenteriae Isolation and identification methods, refinement of the method of sampling and testing procedures; --- Increasing the PCR method for detection and identification of B. hyodysenteriae body. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine, Huazhong Agricultural University. The main drafters of this standard. Chen Jianjun, Guo star Zengxian Dong, Jin Meilin, Zhang Sixi, Huang Sheng, Pinghan Li, Zhao Hui, Contour, Zhang Asia. This standard replaces the standards previously issued as follows. --- SN/T 1207-2003. Swine dysentery quarantine Technical Specifications

1 Scope

This standard specifies the clinical diagnosis of swine dysentery, swine and microscopic tissue sections B. hyodysenteriae Isolation and identification methods. This standard applies to the diagnosis of quarantine of swine dysentery.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety GB/T 19495.3 GMO detection of nucleic acid extraction and purification method

3 Abbreviations

The following abbreviations apply to this document. SD (swinedysentery). swine dysentery Bh (brachyspirahyodysenteriae). B. hyodysenteriae body Bi (brachyspirainnocens). non-pathogenic Brachyspira Bp (brachyspirapilosicoli). colon pilus-like Brachyspira

4 clinical diagnosis

After infection SD pigs exhibited clinical and pathological symptoms most of the more typical for this disease quarantine can be combined with herd epidemics , Clinical symptoms and pathological autopsy results on whether the pigs infected with the disease make a preliminary diagnosis, specific methods see Appendix A.

5 Laboratory diagnosis

5.1 microscopic colon tissue sections 5.1.1 Material preparation 5.1.1.1 Equipment. tissue slicer, biological microscopes, dyeing tanks, water bath, incubator, slides. 5.1.1.2 Reagents. 10% formalin, 70% ethanol, 95% ethanol, ethanol, xylene, paraffin sections, Gram stain solution or Ji Farm Sa staining solution. 5.1.2 Sample Collection and Processing 5.1.2.1 pigs necropsy, lesions take the large intestine paragraph (cecum, colon and rectum front), refrigerated inspection. 5.1.2.2 cut about 1cm2 sample segment set colorectal lesions in 10% formalin fixed at least 3h ~ 4h. ......

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