SN/T 1202-2010 English PDF

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SN/T 1202-2010: Protocol of qualitative polymerase chain reaction for detecting genetically modified plant components in food
Status: Valid

SN/T 1202: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1202-2010	449	Add to Cart	3 days	Protocol of qualitative polymerase chain reaction for detecting genetically modified plant components in food	Valid
SN/T 1202-2003	479	Add to Cart	4 days	Protocol of the qualitative polymerase chain reaction for detecting genetically modified plant components in food	Obsolete

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Basic data

Standard ID: SN/T 1202-2010 (SN/T1202-2010)
Description (Translated English): Protocol of qualitative polymerase chain reaction for detecting genetically modified plant components in food
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.050
Word Count Estimation: 17,126
Date of Issue: 2010-11-01
Date of Implementation: 2011-05-01
Older Standard (superseded by this standard): SN/T 1202-2003
Quoted Standard: GB/T 6682; GB/T 19495.2; GB/T 19495.3; GB/T 19495.7; GB/T 27025; SN/T 1204
Regulation (derived from): National Quality Inspection (2010) 582
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the transgenic plant food ingredients Qualitative PCR detection method. This standard applies to soybeans, corn, tomatoes, potatoes, rice, wheat and other agricultural products and processed products as raw materials, semi-finished goods and finished food transgenic soybean (Roundup Ready), maize (176, Btll, Mon810, T14/T25, GA21, CBH351), tomato (1345-4, 351 N, 5345, 8338, FLAVR SAVR, BioScien, 8805R, Zeneea), potato (NewLeaf Y, NewLeaf Plus, NewLeaf), rice (Bt63), wheat ingredients Qualitative PCR Detection. This standard applies to semi-finished and salty food product does not include condiments and edible oils and fats. This standard can be detected in soybean, corn, tomatoes, Ma Qian potato, rice and wheat as the main raw materials to produce 0. 1% of genetically modified foods.

SN/T 1202-2010: Protocol of qualitative polymerase chain reaction for detecting genetically modified plant components in food

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of qualitative polymerase chain reaction for detecting genetically modified plant components in food People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1202-2003 Food qualitative transgenic plant components PCR detection method Issued on. 2010-11-01 2011-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1202-2003 "gene plant components Qualitative PCR Detection of food transit." This standard compared with SN/T 1202-2003, the main technical changes are as follows. --- Standardized terminology and way of expression; --- Adjust the standard scope (2003 edition Chapter 1; Chapter 1 of this edition), delete the original rapeseed oil and cottonseed oil Food materials, food additives removed, increasing the rice and wheat as raw food; --- Adjusted normative references (2003 edition Chapter 2; Chapter 2 of this edition), using GB/T 19495 series of standards; --- Preparation of test sample treatment (2003 Version 6.4.1; 6.4.1 edition) way of expression rearranged, an increase of a particular type Pretreatment method of samples; --- Food in DNA extraction, using some of CTAB method (2003 edition 6.4.2.1 and 6.4.2.2; Excerpts 6.4.2.1) the method of GB/T 19495.3 prescribed; --- Food in nucleic acid quantification (2003 Version 6.4.3; 6.4.3 edition) using the method GB/T 19495.3 prescribed; --- Modify qualitative PCR detection step PCR amplification reaction type (2003 Version 6.4.4; 6.4.4 edition), deleted Fluorescent PCR method (incorporation assay), an increase of real-time PCR method, and the result is determined in step (2003 Version 6.5; this Version 6.5) a corresponding increase in the real-time PCR method for the detection result of the judgment; --- Ordinary PCR amplification reaction and the result of the judgment (2003 edition 6.3.14,6.4.4,6.5, Tables 1 and 4; Excerpts 6.3.20,6.4.4.1,6.5.1, Table 1, Table 3 and Table 4) way of expression rearranged, modified the reaction system, increasing the primer Sequence, and modify the confirmatory test methods. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine. The main drafters of this standard. High East Wei, Li Danning, Yonghong, Zhong Yuqing Zhang Jun, Dong Jie, Xu Baoliang, Chen Yuan tree, Xie Pei heart. This standard replaces the standards previously issued as follows. --- SN/T 1202-2003. Food qualitative transgenic plant components PCR detection method

1 Scope

This standard specifies the qualitative PCR detection of transgenic food plant components. This standard applies to soybeans, corn, tomato, potato, rice, wheat and other agricultural products and processed products as raw materials to produce food Semi Finished products and finished products GM soy (RoundupReady), corn (176, Bt11, Mon810, T14/T 25, GA21, CBH351), Fan Eggplant (1345-4,351N, 5345,8338, FLAVRSAVR, BioScien, 8805R, Zeneca®), potato (NewLeaf® Y, NewLeaf®Plus, NewLeaf®), rice (Bt63), wheat ingredients qualitative PCR testing. This standard applies to foods and semi-finished products Product does not include condiments and edible oils and fats. This standard can be detected in soybean, corn, tomato, potato, rice, wheat as the main raw material for the production of genetically modified food products 0.1% ingredient.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 19495.3 GMO detection of nucleic acid extraction and purification method GB/T 19495.7 GMO detection methods of sampling and sample preparation GB/T 27025 testing and calibration laboratories General requirements SN/T 1204 GMO plants and their processed products in real-time PCR qualitative test methods

3 Abbreviations

The following abbreviations apply to this document. BAR (phosphinothricinacetyltransferasegenefromBacillusamyloliquefaciens). starch liquefaction Bacillus Bacteria glufosinate acetyltransferase gene bp (basepair). bp Btc (theconstructspecificDNAsequenceoftransgenicriceeventBt63). transgenic rice lines Bt63 knot Configuration-specific gene sequence CaMV35S (35Spromoterfromcauliflowermosaicvirus). cauliflower mosaic virus 35S promoter CTAB (hexadecyltrimethylammoniumbromide). cetyl trimethyl ammonium bromide CryIA (b) (thecrylAblgeneencodinganinsecticidalcrystalproteinfromBacillusthuringiensis). Encoding Bacillus thuringiensis insecticidal protein crystallization crylAb1 gene, also known as the CryIA (b) gene CryIA (b/c) [asyntheticgeneencodingtheinsecticidalcrystalproteinthroughthefusionofCryIA (B) geneandCryIA (c) genefromBacillusthuringiensis]. encoding Bacillus thuringiensis insecticidal crystalline proteins CryIA (b) And CryIA (c) fusion gene ......

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