SN/T 1197-2016 English PDF

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SN/T 1197-2016: Detection of genetically modified ingredients in oilseed rape--Conventional and real-time PCR methods
Status: Valid

SN/T 1197: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1197-2016	419	Add to Cart	4 days	Detection of genetically modified ingredients in oilseed rape--Conventional and real-time PCR methods	Valid
SN/T 1197-2003	399	Add to Cart	3 days	Protocol of the polymerase chain reaction for detecting genetically modified components in rapeseeds	Obsolete

Similar standards

GB/T 11762   GB/T 11761   SN/T 0803.6   

Basic data

Standard ID: SN/T 1197-2016 (SN/T1197-2016)
Description (Translated English): Detection of genetically modified ingredients in oilseed rape--Conventional and real-time PCR methods
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B33
Word Count Estimation: 18,151
Date of Issue: 2016-12-12
Date of Implementation: 2017-07-01
Older Standard (superseded by this standard): SN/T 1197-2003
Regulation (derived from): State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies): General Administration of Customs

SN/T 1197-2016: Detection of genetically modified ingredients in oilseed rape--Conventional and real-time PCR methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of Transgenic Assay in Rape by PCR and Real - time PCR) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Replace SN/T 1197-2003 Detection of genetically modified components in rapeseed General PCR and real-time fluorescent PCR methods Released on December 12,.2016 2017-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 1197-2003 "Qualitative PCR detection method for transgenic components in rapeseed". The main technical differences between this standard and SN/T 1197-2003 are as follows. --- Modified the standard Chinese name and English name; --- Added real-time fluorescent PCR detection method; --- Increased strain-specific detection methods. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard is mainly drafted by. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Pan Liangwen, Li Xiang, Lu Rong, Yang Jielin, Liu Yueming, Gao Qin. The previous versions of the standards replaced by this standard are. ---SN/T 1197-2003. Detection of genetically modified components in rapeseed General PCR and real-time fluorescent PCR methods

1 Scope

This standard specifies the screening of transgenic components in rapeseed and its processed products, gene-specific and strain-specific common PCR and real-time fluorescence. Optical PCR detection method. This standard applies to the screening of transgenic components, gene specificity and strain-specific qualitative detection in rapeseed and its processed products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing SN/T 1194 Detection and sampling method for genetically modified components of plants and their products 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Transgenic transgene Functional DNA sequences derived from other species that are not native to the species, through bioengineering techniques, allowing them to enter the species Line expression in order to obtain new species characteristics for the species. 3.1.2 Real-time fluorescence PCR real-timepolymerasechainreaction Real-time fluorescent polymerase chain reaction. Refers to the addition of fluorophores in the polymerase chain reaction system, using real-time fluorescence signal accumulation The entire PCR process is monitored and the intensity of the fluorescent signal directly reflects the number of templates. 3.1.3 Endogenous gene A gene with a constant copy number and no display of allelic changes in the species is detected. This gene can be used to determine species specificity. 3.1.4 Exogenous gene Other biological genes transferred using bioengineering techniques allow the organism to exhibit new biological traits. 3.1.5 Ct value cyclethreshold The number of cycles experienced by the fluorescent signal in each reaction tube reaching the set domain value. ......

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