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SN/T 1193-2003 English PDF

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SN/T 1193-2003: General requirements for the laboratories for gene detection and identification
Status: Obsolete
Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 1193-2003279 Add to Cart 3 days General requirements for the laboratories for gene detection and identification Obsolete

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Basic data

Standard ID: SN/T 1193-2003 (SN/T1193-2003)
Description (Translated English): General requirements for the laboratories for gene detection and identification
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: C44
Word Count Estimation: 7,782
Date of Issue: 2003-03-17
Date of Implementation: 2003-09-01
Regulation (derived from): National recognition Branch (2011) 63
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies: general requirements GMO testing laboratories, quality control and quality assurance. This standard applies to: GMO testing laboratories construction and quality control.

SN/T 1193-2003: General requirements for the laboratories for gene detection and identification

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
General requirements for the laboratories for gene detection and identification Book of the People's Republic of China Entry and Exit Inspection and Quarantine Genetic testing laboratory technical requirements Released on.2003-03-17 2003-09-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Animal and Plant Quarantine Laboratory of the General Administration of Quality Supervision, Inspection and Quarantine, Shenzhen Entry and Exit Inspection of the People's Republic of China Quarantine Bureau, Guangzhou Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Zhu Shuifang, Chen Hongyun, Huang Wensheng, Yan Wen, Zhang Guiming, Cao Jijuan. This standard is the industry standard for inspection and quarantine issued for the first time. Genetic testing laboratory technical requirements

1 Scope

This standard specifies the general requirements, quality control and quality assurance of genetic testing products testing laboratories. This standard applies to the construction and quality control of genetic testing products testing laboratories. 2 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this standard. 2.1 Genetically modified products, GMOs; biotechnologymodifiedproducts Also known as biotechnology products. Refers to the use of genetic engineering techniques or other modern biotechnology to alter the genome of animals, plants, and micro-organisms. Things and their products. 2.2 Polymerase chain reaction, referred to as PCR. Use a pair of oligonucleotide primers, four deoxyribonucleotides (dNTPs), at the appropriate temperature The process of artificially synthesizing specific template DNA fragments in vitro under the catalysis of ionic conditions, catalysis by heat-resistant DNA polymerase. 2.3 In the traditional PCR reaction system, a fluorescent-labeled hybridization probe specific for amplification template is added, and on a conventional PCR instrument. A fluorescent signal detection system has been added to allow the specificity and quantity detection of PCR amplification products to be synchronized with PCR amplification. Real-time fluorescence There are many kinds of PCR probes, and molecular beacons, Taqman probes, and hybrid double probes are commonly used. 2.4 A special DNA hydrolase that only hydrolyzes DNA that is doped with dUTP. The anti-pollution condition of this enzyme is. during the PCR amplification process Replace dTTP with dUTP; treat PCR amplification reaction solution with UNG enzyme before PCR starts; high temperature (95 ° C) under alkaline conditions Split DNA. 2.5 Abbreviations 2.5.1 PCR. polymerase chain reaction, polymerase chain reaction referred to as PCR. 2.5.2 DNA. deoxyribonucleic acid, deoxyribonucleic acid. 2.5.3 dNTP. deoxyribonucleosidetriphosphate, deoxynucleotide triphosphate. 2.5.4 UNG. uracil-DNAglycosylase, uracil DNA-glycosylase. 2.5.5 dATP. deoxyadenosinetriphosphate, deoxyadenosine triphosphate. 2.5.6 dTTP. deoxythymidinetriphosphate, deoxythymidine triphosphate. 2.5.7 dUTP. deoxyuridinetriphosphate, deoxyuridine triphosphate. 2.5.8 bp. basepair, base pair.
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