SN/T 1172-2015 English PDFSN/T 1172: Historical versions
Basic dataStandard ID: SN/T 1172-2015 (SN/T1172-2015)Description (Translated English): (Chicken leukemia quarantine technical specifications) Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Date of Issue: 2015-02-09 Date of Implementation: 2015-09-01 Older Standard (superseded by this standard): SN/T 1172-2003 Regulation (derived from): State-Quality-Inspection-Accreditation [2015] 59 Issuing agency(ies): General Administration of Customs SN/T 1172-2014: (Chicken leukemia quarantine technical specifications)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.(Chicken leukemia quarantine technical specifications) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Replace SN/T 1172-2003 Chicken leukemia quarantine technical specification Released on.2014-11-19 2015-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 1172-2003 "Agar leukemia detection method agar immunodiffusion test". Compared with SN/T 1172-2003, the main technical changes of this standard are as follows. --- Increased isolation and identification of chicken leukemia virus; --- Increased polymerase chain reaction; --- Increased fluorescence quantitative polymerase chain reaction; --- Increased enzyme-linked immunosorbent assay. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine. The main drafters of this standard. Ye Yiyou, Yang Junxing, Cao Yufu, Hu Yunfa, Chen Bing, Sun Jie, Tao Hong, Tang Jinming, Liu Jianli, Xie Xiaolu, Lv Jianqiang, Lu Tiekang, Hua Qunyi. The previous versions of the standards replaced by this standard are. ---SN/T 1172-2003. Chicken leukemia quarantine technical specification1 ScopeThis standard specifies the isolation and identification of chicken leukemia virus, polymerase chain reaction, fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay Adsorption test, agar diffusion test detection technology. This standard applies to the quarantine of chicken leukemia.2 Normative referencesThe following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 2123 Specification for the collection, transportation and preservation of animal quarantine samples for entry and exit3 AbbreviationsThe following abbreviations apply to this document. ALV. chicken leukemia virus ALV-J. J subgroup chicken leukemia virus AGID. Agar diffusion test BSA. Bovine Serum Albumin CEF. chicken embryo fibroblast ELISA. enzyme-linked immunosorbent assay PBS. phosphate buffer PCR. polymerase chain reaction TMB. 3,3',5,5'-tetramethylbenzidine4 Virus isolation and identification4.1 Reagents and materials 4.1.1 Main reagents. DMEM cell culture medium, 0.25% trypsin, penicillin (100000 IU/mL), streptomycin (100000 IU/mL), Fetal bovine serum, cell lysate. 4.1.2 Main equipment and equipment. cell culture incubator, inverted microscope, microplate reader, centrifuge, grinder, cell culture flask. 4.1.3 Cells and cell lines. chicken embryo fibroblasts (see Appendix A), chicken embryo fibroblasts spontaneous immortal (DF1 cell line). 4.2 Collection, storage and transportation of samples Mainly collect chicken whole blood, tissue samples (spleen, liver and kidney) or throat swabs, cloaca swabs. Sample storage and shipping The SN/T 2123 operates. 4.3 Sample processing 4.3.1 Whole blood. Collect whole blood samples, put them into the test tube with anticoagulant, mix gently; place the test tube upright at room temperature or 37 °C incubator ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1172-2015_English be delivered?Answer: Upon your order, we will start to translate SN/T 1172-2015_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.Question 2: Can I share the purchased PDF of SN/T 1172-2015_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 1172-2015_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.Question 3: Does the price include tax/VAT?Answer: Yes. 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Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
