SN/T 1165.2-2002 English PDF

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SN/T 1165.2-2002: Protocol of agar gel immuno-diffusion test for bluetongue
Status: Valid

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SN/T 1165.2-2002	179	Add to Cart	2 days	Protocol of agar gel immuno-diffusion test for bluetongue	Valid

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Basic data

Standard ID: SN/T 1165.2-2002 (SN/T1165.2-2002)
Description (Translated English): Protocol of agar gel immuno-diffusion test for bluetongue
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 5,570
Date of Issue: 2002-11-25
Date of Implementation: 2003-05-01
Quoted Standard: GB/T 6682-1992
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the agar immunodiffusion test for bluetongue. This standard applies to animals bluetongue antibodies for the detection and diagnosis.

SN/T 1165.2-2002: Protocol of agar gel immuno-diffusion test for bluetongue

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of agar gel immuno-diffusion test for bluetongue People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Blue tongue disease agar immunodiffusion test operation procedure Released on November 11,.2002 2003-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This method refers to the OIE standard ("OIE Diagnostic Test and Vaccine Standards Manual",.1996, third edition) method, and according to China has developed many years of research experience in this field. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Jia Jianjun, Dan Youmin, Xu Zizhong, Zhao Yanrun. This standard is the industry standard for inspection and quarantine issued for the first time. Blue tongue disease agar immunodiffusion test operation procedure

1 Scope

This standard specifies the operating procedures for the blue tongue disease agar immunodiffusion test. This standard applies to the detection and diagnosis of animal bluetongue antibodies.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)

3 Abbreviations

The following abbreviations apply to this standard. 3.1 AGIDTest. Agar immunodiffusion test. 3.2 BT. Bluetongue. 3.3 BTV. Bluetongue virus.

4 Principle

The molecular mass of antigens and antibodies is generally below 2 × 105 U, and the antigen and its specific antibody are in the gel from high concentration to low. When the concentration region diffuses, the resistance is small and is basically in the form of free diffusion. Due to the molecular mass, structure, shape and shape of different antigen molecules The amount of charge is different, so the diffusion coefficient is different and the diffusion speed in the gel is different. When the antigen and the corresponding antibody are diffused in the gel The medium meets to form an antigen-antibody complex, and if the ratio of the two is appropriate at the meeting, the largest complex is formed. Due to the molecular mass of the complex The amount increases, the particles increase, and thus no longer continue to diffuse to produce a precipitate, which exhibits a linear or band shape, and the linear or band-like "specificity" The barrier is called the immunoprecipitation line or the immunoprecipitation zone. It can be based on the presence of this precipitation zone between the antigen and the positive serum, and the antigen and the serum to be tested. Whether or not precipitation occurs between them determines whether or not antibodies are present in the serum to be tested.

5 reagents and materials

5.1 Antigen. BTV17 international standard strain introduced from the National Veterinary Laboratory of the United States, refer to the antigen preparation method of the laboratory Preparation, for group-specific antigen, suitable for quarantine of various types of bluetongue, provided by the designated unit. 5.2 Standard positive serum. using the international standard strain of blue tongue disease introduced from the National Veterinary Laboratory of the United States, according to the conventional high serum-free preparation method Preparation, agar diffusion titer of 1.8 or more, provided by the designated unit. 5.3 Negative serum. Serum without BTV antibody, provided by the designated unit. 5.4 Plate. Ordinary plate. 5.5 Micropipette. ordinary micro pipette. 5.6 Micro dripper. Match the dripper with the pipette. 5.7 Erlenmeyer flask. Ordinary heatable flask. ......

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