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SN/T 1165.1-2002 English PDF

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US$259.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 1165.1-2002: Protocol of competitive ELISA for bluetongue
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1165.1-2002	259	Add to Cart	3 days	Protocol of competitive ELISA for bluetongue	Valid

Similar standards

GB/T 20365 SN/T 1353 NY/T 909 SN/T 1168 SN/T 1162 SN/T 1172

Basic data

Standard ID: SN/T 1165.1-2002 (SN/T1165.1-2002)
Description (Translated English): Protocol of competitive ELISA for bluetongue
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Word Count Estimation: 8,811
Date of Issue: 2002-11-25
Date of Implementation: 2003-05-01
Quoted Standard: GB/T 6682-1992
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary: This standard specifies the bluetongue competitive enzyme-linked immunosorbent assay method. This standard applies to ruminant bluetongue antibodies.

SN/T 1165.1-2002: Protocol of competitive ELISA for bluetongue

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of competitive ELISA for bluetongue People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Blue tongue disease competition enzyme-linked immunosorbent assay protocol Released on November 11,.2002 2003-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

BLuetongue competition enzyme-linked immunosorbent assay (C-ELISA) is based on the International Organization for Animal Disease (OIE) "Mammal, poultry Developed on the basis of the Diagnostic Test and Vaccine Standards Manual for Bees A and B Diseases and Practical Experience. Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Hua Qunyi, Zhou Xiaoli, Xu Zizhong, Dong Jun. This standard is the industry standard for inspection and quarantine issued for the first time. Blue tongue disease competition enzyme-linked immunosorbent assay protocol

1 Scope

This standard specifies the competitive enzyme-linked immunosorbent assay for blue tongue disease. This standard applies to ruminant bluetongue antibody detection.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)

3 Abbreviations

The following abbreviations apply to this standard. 3.1 BTV. Bluetongue virus. 3.2 C-ELISA. Competitive enzyme-linked immunosorbent assay. 3.3 McAb. monoclonal antibody. 3.4 PI. percent inhibition. 3.5 goat anti-mouse IgG-HRP. horseradish peroxidase (HRP)-labeled goat anti-mouse IgG. 3.6 OD value. optical density value.

4 Principle

The test serum and BTV group-specific monoclonal antibody (McAb) were added to the wells of the ELISA plate coated with BTV-VP7 antigen at 37 ° C. Under the reaction, the two compete for binding to the corresponding specific coating antigen in the well, and the enzyme-labeled goat anti-mouse IgG-HRP is added to wash away the unbound anti-antigen. Body, then add substrate to develop color. The OD value of the reactant was measured using a microplate reader, and the percentage of inhibition (PI) was calculated to determine the test result.

5 reagents and materials

5.1 coating antigen. Bluetongue virus VP7 antigen, provided by the designated unit. 5.2 Serum. Blue-tongue standard strong positive, weak positive serum, negative serum, provided by the designated unit. 5.3 Serum to be tested. bovine serum was inactivated at 56 ° C ~ 57 ° C for 30 min, and goat serum was inactivated at 58 ° C ~ 59 ° C for 30 min. 5.4 Bluetongue virus group-specific monoclonal antibodies. provided by the designated unit. 5.5 Sheep anti-mouse IgG-HRP. diluted according to the working concentration specified in the instructions. 5.6 Coating solution, washing solution, diluent, substrate solution, stop solution. See informative Appendix A. 5.7 Water. It meets the secondary water specification of the analytical laboratory of GB/T 6682-1992.

6 instruments and equipment

6.1 Microplate reader. 6.2 Removable 96-well microtiter plate. ......

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Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of SN/T 1165.1-2002_English be delivered?

Answer: Upon your order, we will start to translate SN/T 1165.1-2002_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of SN/T 1165.1-2002_English with my colleagues?

Answer: Yes. The purchased PDF of SN/T 1165.1-2002_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.