SN/T 1164.3-2006 English PDF

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1164.3-2006	279	Add to Cart	3 days	Protocol of ELISA for infectious bovine rhinotracheitis	Obsolete

Similar standards

GB/T 20365   SN/T 1353   NY/T 909   SN/T 1168   SN/T 1162   SN/T 1164.1   

Basic data

Standard ID: SN/T 1164.3-2006 (SN/T1164.3-2006)
Description (Translated English): Protocol of ELISA for infectious bovine rhinotracheitis
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B41
Classification of International Standard: 11.220
Word Count Estimation: 7,714
Date of Issue: 2006-01-26
Date of Implementation: 2006-08-16
Quoted Standard: GB/T 6682
Regulation (derived from): Industry Standard Notice 2006 No. 4 (total of 76)
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the infectious bovine rhinotracheitis ELISA method. This standard applies to infectious bovine rhinotracheitis antibodies.

SN/T 1164.3-2006: Protocol of ELISA for infectious bovine rhinotracheitis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of ELISA for infectious bovine rhinotracheitis Book of the People's Republic of China Entry and Exit Inspection and Quarantine Bovine infectious rhinotracheitis enzyme-linked immunosorbent assay Operating procedure Released on.2006-01-26 Implementation of.2006-08-16 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 1164 is divided into four parts. --- Operational procedures for the isolation of bovine infectious rhinotracheitis virus; --- Bovine infectious rhinotracheitis trace serum neutralization test procedures; --- Bovine infectious rhinotracheitis enzyme-linked immunosorbent assay operating procedures; --- Bovine infectious rhinotracheitis virus detection and identification method PCR method. This part is the third part of SN/T 1164. Appendix A of this section is a normative appendix. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this section. Xiao Ronghai, Zhou Xiaoli, Xu Weijia, Yang Yanxiong, Ying Xuesong. This section is the first industry standard for entry-exit inspection and quarantine. Bovine infectious rhinotracheitis enzyme-linked immunosorbent assay Operating procedure

1 Scope

This part of SN/T 1164 specifies an enzyme-linked immunosorbent assay for bovine infectious rhinotracheitis. This section applies to the detection of bovine infectious rhinotracheitis antibodies.

2 Normative references

The provisions in the following documents become the provisions of this part by reference to this part of SN/T 1164. Quotations with dated , all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouragement is achieved under this section The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to this section. GB/T 6682 Analytical laboratory water specifications and test methods

3 Abbreviations

The following abbreviations apply to this part of SN/T 1164. 3.1 IBR 3.2 ELISA

4 reagents and materials

4.1 Coating antigen. provided by the designated unit. 4.2 Serum. Standard strong positive, negative serum, provided by the designated unit. 4.3 Serum to be tested. bovine serum was inactivated at 56 ° C ~ 57 ° C for 30 min, and goat serum was inactivated at 58 ° C ~ 59 ° C for 30 min. 4.4 Enzyme-labeled anti-antibody. supplied by the designated unit. 4.5 Solution. antigen coating buffer, blocking solution, washing solution, substrate solution and stop solution, see Appendix A. 4.6 Water. Meet the secondary water specifications of the GB/T 6682 analytical laboratory.

5 instruments and equipment

5.1 Microplate reader. 5.2 polystyrene microplate (96-well microtiter plate). 5.3 Single-channel, multi-channel adjustable micropipettes (1μL, 50μL, 100μL, 1000μL), micro dripper. 5.4 Pipette (5mL, 10mL). 5.5 Graduated cylinder (100mL, 1000mL). 5.6 Reagent bottles (50mL, 100mL, 500mL). 5.7 Wash bottle (500 μL). ......

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