SN/T 1148-2019 English PDF

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SN/T 1148-2019: (Cassava single-claw quarantine identification method)
Status: Valid

SN/T 1148: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 1148-2019	319	Add to Cart	4 days	(Cassava single-claw quarantine identification method)	Valid
SN/T 1148-2002	279	Add to Cart	3 days	Plant quarantine. Methods for inspection and identification of cassaca green mite	Obsolete

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NY/T 2288   SN/T 1204   NY 39   SN/T 1144   SN/T 1149   SN/T 1147   

Basic data

Standard ID: SN/T 1148-2019 (SN/T1148-2019)
Description (Translated English): (Cassava single-claw quarantine identification method)
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 15,183
Date of Issue: 2019-09-03
Date of Implementation: 2020-03-01
Older Standard (superseded by this standard): SN/T 1148-2002
Regulation (derived from): Natural Resources Department Announcement No. 7 of 2019
Issuing agency(ies): General Administration of Customs

SN/T 1148-2019: (Cassava single-claw quarantine identification method)

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Cassava single-claw quarantine identification method) ICS 65.020B16 People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard SN/T 1148-2019 replaces SN/T 1148-2002 Method for quarantine and identification of single-clawed mite of cassava Published.2019-2009-03 2020-03-01 implementation Published by the General Administration of Customs of the People's Republic of China ????? ????, ????, ??

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces the quarantine and identification method of single-clawed mite of cassava cassava by SN/T 1148-2002. The main content changes are as follows. --- Added approximate species differences and related search tables; --- Added relevant information such as distribution location; --- Added morphological feature map. This standard is proposed and managed by the General Administration of Customs of the People's Republic of China. This standard is drafted by. Shenzhen Academy of Inspection and Quarantine, Shenzhen Customs, People's Republic of China. The main drafters of this standard. Lu Xiaoyu, Lin Wei, Xu Lang, Yu Daojian, Zhang Weifeng, Zheng Yun. The previous versions of the standards replaced by this standard are. --- SN/T 1148-2002. SN/T 1144-201919 ????? ????, ????, ?? Method for quarantine and identification of single-clawed mite of cassava

1 Scope

This standard is applicable to the quarantine and identification of single-clawed mites carrying cassava in imported plants and their products.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. SN/T 2122 Sampling Methods for Entry and Exit Plants and Plant Products Quarantine

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 3.2 Horny plate on the back of the body. 3.3 3.4 Collective term for setae on the back of the body. Bristles born on the back of the limbs. 3.5 3.6 3.7 It is distributed in the body and appendages, it is slender, the tip is thin, the wall is thick, it is slightly fluffy and has tactile function. 3.8 SN/T 1144-201919 ????? ????, ????, ?? 3.9 The thick and long part is sensation hair, and the thin and short base hair is tentacle hair. 3.10 The ends of the hairs swell and grow on the crotch claws or interclaw processes. It can secrete mucus and make the mite stick to the surface when walking. 3.11 3.12 A bristle at the end of the fimbriae, thick, cylindrical or conical. 3.13 3.14 3.15 The first row of bristles outside the hind body is usually two. Refers to the shoulder area or setae on the shoulder plate. 3.16

4 Basic Information

See Appendix A for additional information on Monochaete cassava.

5 Method principle

Observe the host leaf at the quarantine site according to the hazards of the single-clawed mite of cassava, obtain the adult mites or obtain them in the laboratory, and dissect them SN/T 1144-201919 ????? ????, ????, ?? The specimens of adult mite slides were observed with a microscope, and the types were determined based on morphological characteristics.

6 Equipment and reagents

6.1 Equipment Magnifying glass, stereo microscope, microscope, micrometer, small writing brush, tweezers, scissors, flat bottom finger tube (size about 1cm in diameter, long 4cm, can also be replaced by plastic tube or other bottle tube), sealable plastic bag, porcelain plate, petri dish, ring needle, absorbent paper, dissection needle, glass slide, Coverslips, alcohol lamp, water bath, beaker, graduated cylinder, suction funnel, label, oven, biological incubator. 6.2 Reagent (see Appendix B for preparation method) 6.2.1 Preservative 75% ethanol or Austenitic preservation solution is used to soak mite specimens. 6.2.2 Nessler's solution For cleaning, softening and transparency of specimens. 6.2.3 Slide mounting solution Huo's Mounting Solution or Etching's Mounting Solution, for mounting slide specimens. 6.2.4 Canadian gum Used to prevent dried slide mounting fluid from becoming soft. 7 On-site quarantine and breeding 7.1 On-site quarantine Random inspection of host plants at the quarantine site shall be carried out in accordance with Sn/T 2122. Use a magnifying glass to observe the main parts, such as cuttings, The young leaves, buds and young stems of the cuttings, and the leaves damaged by the single-clawed mite cassava, have a large number of yellow spots, chlorosis, deformation, blackening, and young stem development. Cassava cuttings and cuttings from areas where cassava monoclaw mites occur, such as Brazil and most countries in Africa, should be carefully checked. Directly with a small brush The mites are provoked and placed in a finger tube containing 75% ethanol, or the mites are collected by tapping, that is, tap the plant with a wooden strip to make the mites fall. Below Mite was collected in a large porcelain plate covered with black paper, and the mite was placed in a finger tube containing 75% ethanol. For samples suspected of carrying mites, In a sealable plastic bag. The above finger tubes and plastic bags are labeled or numbered, and the time, place, host, collector, etc. are recorded and brought back to the actual Laboratory. 7.2 Rearing Suspicious cassava cuttings or cuttings found to be mite-damaged should be kept in an indoor biological incubator for 1 to 2 weeks at a temperature of 25 ° C. ± 2 ℃, humidity is (45 ± 5)%, light time is 15h/d, and then the leaves, buds and young stems of plant samples are mirrored one by one with the help of a stereo microscope Adult mites were obtained.

8 laboratory testing

8.1 Preparation of slide specimens For the preparation of slide specimens, see Appendix C. SN/T 1144-201919 ????? ????, ????, ?? 8.2 Microscopy Place the slide specimen under a microscope to observe and measure, and if necessary, photograph key features.

9 Identification characteristics

9.1 Tetranyidae The spider mites are tiny, female adult mites have reproductive caps and folds on the abdomen, and male mites have special structured penis. Female mites are much smaller; the epidermis is soft and there is no shield on the back; the forefoot usually has 3 pairs of back hairs, and the back half has 10 pairs of back hairs. The number of back hairs will increase The number and position of the pedicles vary according to the genus. The beard must have 5 knots, the tibia has strong claws, and the tarsus has 6-7 bristles. It is bi-hairy, the crotch claws have sticky hairs, with or without sticky hairs between the claws. For a map of the spider mite model, see Appendix D. Common species of spider mites for genus Cassava See Appendix E for the search form. 9.2 Monocladus The dorsal epidermal pattern of the adult mites in the genus Mites is longitudinal between the 3rd pair of middle hairs; the back hairs usually grow on the weak protrusions; The two pairs of typical double hairs are close to each other; there are interclaw processes and no sticky hairs; the interclaw processes split into 3 pairs of needle-shaped hairs; Anal hair 2 to 3 pairs. 9.3 Monochaeta cassava 9.3.1 Adult mites The body is green; the female mite is about 350 μm long, and the male mite is about 230 μm long, including the jaw body 281 μm. The sensory limb extremities are thick and short, less than 1.5 times the width; the front end of the needle sheath is obtuse; the end of the valve groove is spherical. The epidermal striae are obvious, and the epidermis at the back of the forefoot is slightly reticular. before The length of the back of the foot, the back of the back, and the shoulders are equal to the distance between their bases; the length of the hair in the back of the back is about the same as the distance between their bases. 1/2. The foot tibia has 9 tentacles and 1 slender hair, and the tarsus has 5 tentacles and 1 slender hair; the foot tibia has 7 tentacles, 跗 The knot has 3 cilia and 1 slender hair. The male genitalia swelled and formed a globular shape. For male genitalia, see Appendix D. See Tables E and F for the retrieval tables of Monochaete cassava and its approximate species. 9.3.2 Young mites There are 3 pairs of feet; if the mites have 4 pairs of feet, if the mite is smaller than the adult mite, the adult mite has reproductive holes, but the adult mite does not. 9.3.3 Eggs Spherical. 10 result judgment It meets the identification characteristics of 9.3.1, and can be judged to be cassava mite, 9.3.2 and 9.3.3 as reference. 11 Sample Record The identified specimens of the single-headed mites of Cassava should be kept permanently, and the time, place, host, and collector should be noted. SN/T 1144-201919 ????? ????, ????, ??

Appendix A

(Informative appendix) Relevant information of Monochaete cassava A. 1 Geographical distribution Antigua and Barbuda, Dominican Republic, United States, Mexico, Trinidad and Tobago, Australia, Northern Mariana Islands, Micronesia, Mali, Guinea, Sierra Leone, Liberia, Ghana, Togo, Benin, Niger, Nigeria, Cameroon, Chad, China Africa, Sudan, Ethiopia, Somalia, Kenya, Uganda, Tanzania, Rwanda, Burundi, Zaire, Congo, Gabon, Angola, Zambia, Malawi, Zimbabwe, Côte d'Ivoire, Burkina Faso, South Africa, Argentina, Paraguay, Brazil, Puerto Rico, Bolivia, Ecuador Guador, Guyana, Colombia, Malvinas Islands, Peru, Suriname, Venezuela, Uruguay, Chile and other countries. Cassava plantations have been found in Hainan, Guangxi, Guangdong, and parts of Yunnan. A. 2 host plants Cubicaee, Passifloraceae, Passionaceae, Malvacae of Malvaceae, and Rubiaceae of Rubiaceae. A. 3 Biology and transmission The cassava single-clawed mite's life history is divided into five stages. egg, juvenile mite, former nymph, posterior nymph and adult mite. Often spawns on the back of the leaf along the midrib to other leaves Veins or leaf depressions, sucking plant buds, buds, new leaves and young stem juice with acupuncture. In the field, its spreading methods are crawling and spreading with the wind; and its main spreading path is human activity and parasite plant transmission. A. 4 The hazards of Monochaete cassava A large number of yellow spots appeared on the blocked roots of the plant after the injury, and then expanded into a mosaic pattern, which became deformed. When severely injured, the leaves withered yellow Shedding, green shoots loss, stems appear curved, scars, gradually dry and rough, brown, and finally the plants die and the roots rot. SN/T 1144-201919 ????? ????, ????, ??

Appendix B

(Informative appendix) Reagent preparation method B. 1 Preparation method of Austenitic preservation solution Mix 70 mL of ethanol 87 mL, 8 mL of glacial acetic acid, and 5 mL of glycerol. B. 2 Preparation method of Nessler's solution Mix 40g of chloral hydrate, 25mL of distilled water, and 2.5mL of concentrated hydrochloric acid. B. 3 Preparation method of Huo's mounting solution Dissolve 30g of gum arabic in 50mL of distilled water. After it is completely dissolved, put it in a water bath at 40 ℃ ～ 50 ℃ and add.200g. Trichloroacetaldehyde hydrate, stir until dissolved, add 20g of glycerin, and finally filter with a suction funnel or fine cotton cloth. B. 4 Preparation method of Ai Cai's mounting solution Add 40mL to 60mL of distilled water in 10g of polyvinyl alcohol, heat on a 100 ° C water bath, stir until completely dissolved, and then add 85 to 92% lactic acid 35mL and 10mL glycerin, stir well, cool to 40 ℃ ～ 50 ℃, add 25mL 1.5% phenol aqueous solution And 20 g of trichloroacetaldehyde hydrate, and finally filter with an air extraction funnel or fine cotton cloth. Note. Unless otherwise specified, all reagents are of analytical grade. SN/T 1144-201919 ????? ????, ????, ??

Appendix C

(Informative appendix) Preparation of slide specimens Use a circular needle to pick the mite specimen from the preservation solution, put it in a Petri dish containing Neisser's solution, and soak it for 24 hours. After soaking mites Pick it up on absorbent paper and use a small brush to dip the Ness solution to clean the specimen. Place a drop of mounting fluid in the center of a clean glass slide. It is advisable to just cover the coverslip. Use a dissecting needle dipped in the mounting solution to quickly remove the mite specimen from the absorbent paper into the glue solution. Finish the mite Immerse in the bottom layer of the mounting fluid instead of floating on the surface. Posture under a stereo microscope so that the mites have their backs up and their legs extended. When placed in a mounting medium, the male penis can be fully displayed. Apply a small amount of mounting solution to the center of the coverslip and clamp the cover with tweezers The slide is pressed against the slide in parallel, so that the mounting liquid on the cover glass and the mounting agent on the slide are in parallel contact, and the mounting liquid is evenly spread around. will The prepared slide is observed under a stereo microscope. If the left and right eyes of the front and side male mites are completely overlapped, the position of the male mite penis is right Suitable. Place the slide on the flame of the alcohol lamp and move it to heat. When the mounting fluid on the slide is boiling, remove it immediately to allow the mite body to finish. Fully stretched and transparent. The slides were placed in an oven at 55 ° C for 2-7 days until they were completely dry. In order to prevent the mounting fluid from getting damp when it is wet, cover the glass with Canadian glue. The film was sealed around. SN/T 1144-201919 ????? ????, ????, ??

Appendix D

(Informative appendix) Tetranychus mite model and male genus characteristic diagram Figure C. 2 The ventral view of spider mite (quoted from Economic Entomology) SN/T 1144-201919 ????? ????, ????, ?? Figure C. 3 Jaw body of spider mite (quoted from Economic Entomology) Figure C. 5 Characteristic map of male genitalia of Monosauria cassava (imitation of J. GUTIERERY, 1978) SN/T 1144-201919 ????? ????, ????, ??

Appendix E

(Informative appendix) Retrieval Form for Common Tetranychus in Cassava E. 1 Retrieval form of common spider mite species that harm cassava plants The hairs of the foot iliac joint are normal; Terminal body with 1 pair of anal side hairs 3 SN/T 1144-201919 ????? ????, ????, ??

Appendix F

(Informative appendix) Retrieval form of Monochaeta cassava and its approximate species F. 1 Retrieval form of Monochaete cassava and its approximate species The back stripes are not connected; there may be a slight reticulation at the back; foot I tibial has 8 or 9 tentacles and 1 sensory hair 2 2． The hair in the back of the back body is about 1/2 of the length of the hair at the base; the tibial portion of the foot has 9 tentacles and 1 slender hair. All back hairs are of equal length; foot I tibial has 8 or 9 tentacles and 1 slender hair 3 3． Body hair is long, thick, jagged, and swollen at the ends. The length of the back hair is approximately the same except for the shorter front half body shoulder hair and shoulder hair which is slightly shorter; foot I SN/T 1144-201919 ????? ????, ????, ?? references [1] RenataS Mendonza, DeniseNavia, IvonRDini, etal. SoupAmerican Spicer Mittes. Newtownslocolics [J]. Jounalof Scene Science,.2011, 11 (5). 1-18. [2] J. Gutierereｒ. TheThessavaGreenMitinAfrica. OneTorse Spices? (Acari. Tetranychi- dae) [J]. Experimental & Applicable Carel, 1987, 3. 163-168. [3] Carlos Flechtman. Thecassava mitcomplex. taxonomy and difiction [J]. Proceedings Cascade Prospecting Worldshop, 1977, 11. 143-153. [4] Wang Huifu, et al. Economic Entomology (Book 23, Tetranychusidae) [M]. Beijing. Science Press, 1981. 19-28, 88. [5] Marx Pei, Shen Zhaopeng, Chen Xiwen, etc. Chinese agricultural mites [M]. Shanghai. Science and Technology Press, 1984. 6-94. SN/T 1144-201919 ????? ????, ????, ?? ......

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