SN/T 1139-2002 English PDFUS$239.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1139-2002: Serological detection method of broad bean stain comovirus Status: Obsolete
Basic dataStandard ID: SN/T 1139-2002 (SN/T1139-2002)Description (Translated English): Serological detection method of broad bean stain comovirus Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B16 Word Count Estimation: 6,668 Date of Issue: 2002-08-02 Date of Implementation: 2003-01-01 Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China Summary: This standard specifies the broad bean stain virus DAS-ELISA detection method. This standard applies to broad bean seeds, seedlings and other crops bean stain virus detection. Beans and other crop seed testing within the virus, which is subject to germinate seeds or seedlings before planting and the formation of detection. SN/T 1139-2002: Serological detection method of broad bean stain comovirus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Serological detection method of broad bean stain comovirus People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard stew - Broad bean staining virus serological detection method ┇┄━┄━┉┉┄┃│┉┄┄┇┄┃┈┉┃┄│┄┋┇┊ ┈ ┐┐ release Implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Nanjing Agricultural University. The main drafters of this standard. Tao Tingdian, Xu Zhigang, Yi Jianping, Yin Liping, Yang Cuiyun. This standard was first published Inspection and quarantine industry standards. stew - Broad bean staining virus serological detection method range This standard specifies the DAS-ELISA method for detection of broad bean staining virus. This standard applies to the detection of broad bean staining virus in broad bean seeds, seedlings and other related crops. Detection of broad beans and other crop seeds When the virus is germinated or planted and formed into seedlings, it is tested. Abbreviations The following abbreviations apply to this standard. DAS-ELISA double antibody sandwich enzyme-linked immunosorbent assay PBS phosphate buffer 10×PBS 10-fold concentration PBS mother liquor for rapid preparation of PBS buffer PBST PBS containing Tween-20 SEB sample extract IgG refers to purified broad bean staining virus antibody IgG Coating-IgG refers to the antibody IgG used to coat the broad bean staining virus of the enzyme-linked plate. IgG-conjugate refers to antibody IgG of broad bean-stained virus labeled with alkaline phosphatase CB coating buffer Method summary and its basis The broad bean staining virus was discovered by Lloyd in 1965 on the British broad bean. It is a globular virus with a diameter of about 28 nm. Mainly distributed in Africa and Europe and Asia. The broad bean staining virus belongs to the genus Comoviridae and the cowpea mosaic virus (co- Movirus). The broad bean-stained virus has moderate-intensity immunogenicity, and the antibodies produced can be used as various serological reactions. Sensible leaf juice The liquid contains more virions and can be detected by serological reaction. DAS-ELISA in serological methods is a stability A good and specific method, this standard uses the DAS-ELISA method widely used at home and abroad to detect the broad bean staining virus. . Enzyme plate. . Adjustable pipettes (2.5μL, 10μL, 100μL,.200μL, 1000μL each) and corresponding tips. . 12-well.200μL adjustable pipette and tip. . Acidity meter. . Adjust the temperature bath or thermostat incubator. . ELISA plate washer (not required by this standard). . Microplate reader. . A micro sample juicer (with a micro sample juicer, hand-grinding with a mortar). . Measuring cylinder (10 mL, 20 mL, 50 mL, 100 mL, 500 mL, 1000 mL each). . Analytical balance. Accuracy 1 stew 10000g. . Reagent bottles (10 mL, 50 mL, 100 mL, 500 mL, 1000 mL each). stew - ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1139-2002_English be delivered?Answer: Upon your order, we will start to translate SN/T 1139-2002_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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