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Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1129-2015: Quarantine protocol for bovine viral diarrhea/mucosal disease Status: Valid SN/T 1129: Historical versions
Basic dataStandard ID: SN/T 1129-2015 (SN/T1129-2015)Description (Translated English): Quarantine protocol for bovine viral diarrhea/mucosal disease Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: B41 Classification of International Standard: 11.220 Word Count Estimation: 15,153 Date of Issue: 2015-09-02 Date of Implementation: 2016-04-01 Older Standard (superseded by this standard): SN/T 1129-2007; SN/T 1905-2007 Quoted Standard: GB/T 6682; GB/T 19495.2 Regulation (derived from): AQSIQ Announcement 2015 - Batch 3 - 103 Industry Standards Issuing agency(ies): General Administration of Customs Summary: This standard specifies the clinical diagnosis of bovine viral diarrhea/mucosal disease, virus isolation and identification, fluorescent antibody test, immunoperoxidase monolayer assay, micro serum neutralization test, antigen capture enzyme-linked immunosorbent assay, reverse transcription - polymerase chain reaction procedures. This standard applies to the import and export of animals and products of bovine viral diarrhea/mucosal disease quarantine. SN/T 1129-2007: Bovine viral diarrhea / mucosal disease quarantine norms---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Bovine viral diarrhea/mucosal disease quarantine norms Exit inspection and quarantine industry standard book People's Republic of China Instead of the SN/T 1129.1 ~ 1129.2-2002 Bovine viral diarrhea/mucosal disease quarantine norms Posted 2007-08-06 2008-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordInstead of the standard SN/T 1129.1-2002 "bovine viral diarrhea/mucosal disease virus micro serum neutralization test procedures" and SN/T 1129.2-2002 "bovine viral diarrhea/mucosal disease virus isolation procedures." The Standard Appendix A and Appendix B are informative. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine of Liaoning People's Republic of China Bureau, People's Republic of China Tianjin Exit Inspection and Quarantine. The main drafters of this standard. Sun Yingjie, Zhang Boqiang, Baiquan Yang, Zhang silver body, Yuan Wenze, Jane Friends Dong Zhizhen, Huolei. This standard replaces the standards previously issued as follows. --- SN/T 1129.1-2002; --- SN/T 1129.2-2002. Bovine viral diarrhea/mucosal disease quarantine norms1 ScopeThis standard specifies the bovine viral diarrhea quarantine norms. This standard applies to bovine viral diarrhea virus isolation and identification, viral antigen detection, virus antibody detection can be used to quarantine the disease, diagnosis Off and epidemiological investigation.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis3 Disease OverviewBovine viral diarrhea (bovineviraldiarrhea, BVD) is a bovine viral diarrhea virus (bovineviraldiarrheavirus, BVDV) causes one kind of diarrhea as the clinical features of infectious diseases that can lead to sustained pregnant cow abortion, stillbirth, teratogenic or newborn calves Infection, which is highly contagious, but mild symptoms and disease, incidence and mortality is low. Mucosal disease (MucosalDisease, MD) also BVDV is a caused by the bovine type of acute oral, gastrointestinal mucosal inflammation, erosions, ulcers characterized by infectious diseases. It and bovine virus Diarrhea is caused by the same pathogen, but the performance of different clinical symptoms, the incidence of animal mouth, especially along the gingival margin erosion can also occur tears And a lot of salivation. Infectious disease is not high, clinical symptoms, low incidence and high mortality. Pathogen, epidemiology, symptoms, onset Mechanism and pathological changes, diagnosis and quarantine, see Appendix A.4 quarantine methods4.1 Isolation and identification of viruses 4.1.1 Instruments Reagents 4.1.1.1 inverted fluorescence microscope. 4.1.1.2 Carbon dioxide incubator. 4.1.1.3 10mL standard cell culture bottles, flat-bottomed 24-well cell culture plate, covered wet box. 4.1.1.4 BVD-free fetal calf serum antibodies, cell dispersion. 4.1.1.5 BVD standard strain (OregonC24 or NADL) and positive serum, BVD fluorescent antibody, BVD enzyme labeled antibody. 4.1.1.6 Cell. Use within three generations or MDBK cells uninfected BVD virus in fetal bovine kidney cells (BK). 4.1.1.7 solutions and cell culture. preparation see Appendix B. 4.1.2 collect samples 4.1.2.1 For cattle, cattle quarantine should be sterile collection of blood or semen. 4.1.2.2 died for suspected bovine viral diarrhea, and blood clots should be collected and mesenteric lymph nodes. 4.1.2.3 for miscarriage, stillbirth cattle quarantine, fetal tissue should be collected. 4.1.3 Processing of samples 4.1.3.1 After the blood serum was separated by a conventional method, a blood clot should thaw three times, and the supernatant precipitated. 4.1.3.2 semen freezing and thawing three times (or sonication), the prescribed dilution, centrifuged and the supernatant. 4.1.3.3 plus 10 times the volume of animal tissue containing 1000IU/mL cell culture medium grind double antibody, centrifuged and the supernatant. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 1129-2015_English be delivered?Answer: Upon your order, we will start to translate SN/T 1129-2015_English as soon as possible, and keep you informed of the progress. 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Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.Question 5: Should I purchase the latest version SN/T 1129-2015?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version SN/T 1129-2015 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. |
