SN/T 0538-2010 English PDF

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SN/T 0538-2010: Determination of spiramycin residues in meat for import and export. Cylinder plate method
Status: Valid

SN/T 0538: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
SN/T 0538-2010	469	Add to Cart	3 days	Determination of spiramycin residues in meat for import and export. Cylinder plate method	Valid
SN/T 0538-1996	RFQ	ASK	3 days	Method for the determination of spiramycin residues in meat for export. Cylinder plate method	Obsolete

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Basic data

Standard ID: SN/T 0538-2010 (SN/T0538-2010)
Description (Translated English): Determination of spiramycin residues in meat for import and export. Cylinder plate method
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B40
Classification of International Standard: 67.120.10
Word Count Estimation: 18,132
Date of Issue: 2010-01-10
Date of Implementation: 2010-07-16
Older Standard (superseded by this standard): SN/T 0538-1996
Quoted Standard: SN/T 1750-2006
Regulation (derived from): National Quality Inspection [2010] No. 13
Issuing agency(ies): General Administration of Customs
Summary: This standard specifies the import and export of meat residue testing in spiramycin saucer sample preparation and assay. This standard applies to export pig meat division of spiramycin residues, can refer to other types of meat used.

SN/T 0538-2010: Determination of spiramycin residues in meat for import and export. Cylinder plate method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of spiramycin residues in meat for import and export. Cylinder plate method Exit inspection and quarantine industry standard book People's Republic of China Replacing SN 0538-1996 Meat Importers spiramycin residues in detection methods Plate method Issued on. 2010-01-10 2010-07-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard replaces SN 0538-1996 "Meat exports in spiramycin residues in detection plate method." This standard compared with SN 0538-1996, the main changes are as follows. --- Modify the standard name; --- The structure and format of the original text of the standard has been revised; --- Regulate the amount of units, symbols of expression; --- The method of preparation of the solution put in Appendix A; --- Deleted the original standard provisions on sampling and inspection lot number; --- Increasing the passage of the test strains and conservation requirements; --- The recovery test data to a range of values. Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard by the People's Republic of China Hubei Exit Inspection and Quarantine is responsible for drafting. The main drafters of this standard. Zhao Hui, Wang Peng, Xu Xinsheng, Wang Zhenhua, Contour, Cengxian Dong, Pinghan Li, Chen Jianjun, Wuwei Liang. This standard replaces the standards previously issued as follows. --- SN 0538-1996. Meat Importers spiramycin residues in detection methods Plate method

1 Scope

This standard specifies the import and export of meat spiramycin residues in sample preparation and assay cup and saucer. This standard applies to import and export pig meat division of spiramycin residues, can refer to the use of other types of meat.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. SN/T 1750-2006 food of animal origin antibiotics residue detection method for microbial inhibition assay

3 Method summary

The residue in the sample by the use of spiramycin buffer Ⅱ (pH9.2) and the extracts were then extracted dichloroethane. Dichloroethane extract After evaporated and the residue buffer Ⅲ (pH8.0) was dissolved to prepare a sample solution. Sample liquid remaining in spiramycin inhibition of Micrococcus luteus growth, yield Health inhibition zone, from the size of the zone of inhibition assay sample liquid residues in meat spiramycin quantitatively using a standard curve.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade, water is distilled water. 4.1 methanol. 4.2 sodium chloride. 4.3 1,2-dichloroethane. 4.4 buffer Ⅰ (pH8.0). See Appendix A, Section A. Chapter 1. 4.5 buffer Ⅱ (pH9.2). See Appendix A, Section A. 2. 4.6 buffer Ⅲ (pH8.0). See Appendix A, Section A. 3. 4.7 Spiramycin standard stock solution. See Appendix A, Section A. 4. 4.8 Spiramycin standard working solution. See Appendix A for the first A. 5. 4.9 enrichment culture medium. See Appendix A, Section A. 6. Save 4.10 with medium. See Appendix A, Section A. 7. 4.11 Calibration medium. See Appendix A, Section A. 8. 4.12 nutrient broth. See Appendix A, Section A. 9. 4.13 Spiramycin Standard. Residue analysis reference substance, CAS No. 8025-81-8. Sealed light, moisture, 4 ℃ refrigerator. Strain and save passage may refer to SN/T 1750-2006 Appendix B performed. 5. Apparatus 5.1 dish. diameter 90mm, bottom smooth, with terracotta cover. 5.2 Oxford Cup. stainless steel, diameter 8mm ± 0.1mm, an inner diameter of 6mm ± 0.1mm, height 10mm ± 0.1mm. ......

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