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SN/T 0535-2016 English PDF

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SN/T 0535-2016: Determination of gossypol in feed for import and export
Status: Valid

SN/T 0535: Historical versions

Standard IDUSDBUY PDFLead-DaysStandard Title (Description)Status
SN/T 0535-2016509 Add to Cart 5 days Determination of gossypol in feed for import and export Valid
SN 0535-1996479 Add to Cart 3 days Method for the determination of gossypol in fodders for export. Liquid chromatographic method Obsolete

Similar standards

GB/T 5519   SN/T 0800.1   LS/T 6103   SN/T 0800.10   SN/T 0491   SN/T 0695   

Basic data

Standard ID: SN/T 0535-2016 (SN/T0535-2016)
Description (Translated English): Determination of gossypol in feed for import and export
Sector / Industry: Commodity Inspection Standard (Recommended)
Classification of Chinese Standard: B20
Word Count Estimation: 22,220
Date of Issue: 2016-08-23
Date of Implementation: 2017-03-01
Older Standard (superseded by this standard): SN 0535-1996
Regulation (derived from): State-Quality-Inspection-Certification (2016)438
Issuing agency(ies): General Administration of Customs

SN/T 0535-2016: Determination of gossypol in feed for import and export

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of gossypol in feed for import and export People's Republic of China Exit Inspection and Quarantine standards Replacing SN 0535-1996 Import and export of feed gossypol determination 2016-08-23 released 2017-03-01 Implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard is drafted in accordance with the planning given in GB/T 1.1-2009. This standard replaces SN 0535-1996 "export feed gossypol test method for liquid chromatography." Compared with SN 0535-1996, except The main technical changes outside the editorial changes are as follows. --- Increase the confirmation method; --- Technical indicators to meet a variety of substrates and limited requirements. Please note that some of this document may be patentable. The issuing agencies of this document do not bear the responsibility of identifying these patents. This standard is proposed and managed by the People's Republic of China Certification and Accreditation Administration. This standard was drafted by Zhejiang Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Inspection and Quarantine Technology Center, Shanghai, People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Zhang Wenhua, Huang Chaoqun, Zou right, Chen Lingling, Yu Zoran, Tong Kai, Chen Li, Tang Hang Yan. This standard replaces the standards previously issued as. --- SN 0535-1996. Import and export of feed gossypol determination

1 Scope

This standard specifies the two methods of determination of gossypol in feed, high performance liquid chromatography (HPLC) and liquid chromatography - mass spectrometry/mass spectrometry (LC-MS/MS method). This standard applies to cottonseed cake (meal), pig feed and gossypol quantitative determination of chicken feed; liquid chromatography - mass spectrometry/mass spectrometry quantitative Confirmed.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods 3 first high performance liquid chromatography (HPLC method) 3.1 principle Samples were extracted with acetone aqueous solution, filtered membrane, using high performance liquid chromatography, external standard method. 3.2 Reagents and materials Unless otherwise specified, all reagents are of analytical grade, water is a water GB/T 6682 regulations. 3.2.1 Acetone. Chromatographic purity. 3.2.2 methanol. Chromatographic purity. 3.2.3 Phosphate. 3.2.4 Acetone - water solution (70 30, volume ratio). Accurately take 70mL acetone (3.2.1) and 30mL water, mix well before use. 3.2.5 0.1% phosphoric acid solution .1mL phosphoric acid dissolved in water, and set to 1L, mix and filter (0.45μm) before use. 3.2.6 gossypol standard .CAS.303-45-7, purity of 90.5%. 3.2.7 gossypol standard stock solution Accurately weigh 10mg (accurate to 0.1mg) gossypol standard in 10mL volumetric flask with acetone (3.2.1) Dissolve and dilute to the mark to prepare a standard stock solution at a concentration of 1 mg/mL and store at 4 ° C in the dark. 3.2.8 microporous membrane .0.22μm, organic. 3.3 Instruments and Equipment 3.3.1 High performance liquid chromatography (HPLC) instrument with UV detector. 3.3.2 electronic balance. Sense of 0.01g and 0.0001g. 3.3.3 Centrifuge speed of not less than 4000r/min. 3.3.4 Shaker. Speed of not less than 300r/min. 3.3.5 vortex mixer. 3.3.6 plastic centrifuge tube .50mL. 3.3.7 mortar.
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