SB/T 10389-2004 English PDF

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SB/T 10389-2004: Determination of sorbic acid in meat and meat products
Status: Obsolete

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Basic data

Standard ID: SB/T 10389-2004 (SB/T10389-2004)
Description (Translated English): Determination of sorbic acid in meat and meat products
Sector / Industry: Domestic Trade Industry Standard (Recommended)
Classification of Chinese Standard: X04
Classification of International Standard: 67.120
Word Count Estimation: 5,515
Date of Issue: 2004-08-04
Date of Implementation: 2004-09-01
Issuing agency(ies): People Republic of China Ministry of Commerce
Summary: This standard specifies: sorbic acid in meat and meat products Determination party, . This standard applies to: Meat and meat products sorbic acid test.

SB/T 10389-2004: Determination of sorbic acid in meat and meat products

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of sorbic acid in meat and meat products ICS 67.120 X04 Record number. 14170-2004 People's Republic of China domestic trade industry standard Determination of sorbic acid in meat and meat products Released on.2004-08-04 2004-09-01 implementation Published by the Ministry of Commerce

Foreword

This standard was proposed by the Ministry of Commerce of the People's Republic This standard was drafted by the Department of Commerce's Slaughter Technology Appraisal Center. The Center for Nutrition and Food Safety of the Chinese Center for Disease Control and Prevention, Hebei Province The Center for Disease Control and Prevention participated in the drafting. The main drafters of this standard. Jin Shesheng, Yang Zuying, Song Shufeng, Han Huixin, Zhang Xinling, Liu Hucheng, Wang Guiji. This standard is entrusted to the Ministry of Commerce's Slaughter Technology Appraisal Center for interpretation. Determination of sorbic acid in meat and meat products

1 Scope

This standard specifies the method for determination of sorbic acid in meat and meat products. This standard applies to the testing of sorbic acid in meat and meat products.

2 Principle

The sorbic acid in the sample was extracted, purified with neutral alumina, and then subjected to high performance liquid chromatography.

3 reagents and materials

The reagents used below are analytically pure reagents unless otherwise specified, and the water is re-distilled water. 3.1 Methanol. excellent grade pure. 3.2 Ammonium acetate. 3.3 Neutral alumina. for chromatography, (100 ~.200) mesh. 3.4 0.02mol/L ammonium acetate solution. 1.54g ammonium acetate was weighed, dissolved in water and diluted to 1L. 3.5 Sorbic acid. 99% (National Center for Standard Materials Research). 3.6 Standard stock solution of sorbic acid. Accurately weigh 25.0mg sorbic acid standard, dissolve it with a small amount of ethanol, dilute with water and dilute to volume 25.0 mL, shake well. This solution contained 1.0 mg of sorbic acid per ml. Stored in the refrigerator for 15 days. 3.7 Standard solution of sorbic acid. Pipette 5.0 mL of standard stock solution of sorbic acid in a 100 mL volumetric flask, dilute to the mark with water, and Shake well. This solution contained 50 μg of sorbic acid per ml. Stored in the refrigerator, valid for 7 days. 3.8 Sorbic acid standard series solution. 1.0, 2.0, 3.0, 4.0, 5.0mL sorbic acid standard intermediate solution in 10mL volumetric flask Add water to the scale and shake well. That is, a standard series solution containing 5.0, 10.0, 15.0, 20.0, 25.0 μg of sorbic acid per ml is obtained.

4 Instruments and equipment

4.1 High Performance Liquid Chromatograph. UV detector. 4.2 Ultrasonic cleaner. 4.3 Vortex mixer. 4.4 Centrifuge. 4.5 Rotate the evaporator. 4.6 Microporous membrane filter.

5 Determination steps

5.1 Sample processing Take.200g sample and grind it, weigh about 1.00g (accurate to 0.01g) sample, put it in 50mL plastic test tube, add 5mL water, spin The mixture was vortexed for 1 min, ultrasonically extracted for 15 min, and then centrifuged at 4000 r/min for 20 min. Column chromatography. use a 10mL syringe, fill a small amount of absorbent cotton at the bottom, and contain 3cm high neutral alumina, first wet the neutral oxygen with water. Aluminum column, when the water surface is close to the top of the column, add the sample extract and let it flow down. When the liquid level of the sample liquid approaches the top of the column, it is eluted with the mobile phase. The eluate was transferred to a 25 mL volumetric flask. At the time of the calibration, the elution was stopped, shaken, and the solution was filtered through a microporous membrane, and the filtrate was subjected to HPLC analysis. 5.2 Chromatographic determination 5.2.1 Liquid Chromatography Reference Conditions a) Column. HypersilODS2, 250mm × 4.6mm, particle size 5μm; ......

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